CoChat
Sign Up
Cited by 25

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Chapter Twenty‐Eight Qualitative and Quantitative Characterization of Autophagy in Caenorhabditis elegans by Electron Microscopy

Sigmond, Timea, Fehér, Judit, Baksa, Attila, Pásti, Gabriella, Pálfia, Zsolt, Takács‐Vellai, Krisztina, Kovács, János, Vellai, Tibor, Kovács, Attila L.·2008·Methods in Enzymology
DOI 10.1016/s0076-6879(08)03228-x
Open this paper in CoChat — free account

Abstract

Caenorhabditis elegans has been introduced relatively late into the field of autophagy with no previous results by classical methods. Therefore, it has to be studied in parallel with both traditional electron microscopy and modern molecular approaches. In general, correct identification of autophagic elements by electron microscopy is indispensable to establish a firm basis for our understanding of the process. The principles and the method for identification, applied also for C. elegans, are summarized first in this article, to facilitate their utilization both for further studies and the analysis of new cell types and to support researchers new to electron microscopy techniques. Studying autophagy in the worm by electron microscopy has required the development of special handling and sampling techniques in addition to overcoming the general technical difficulties due to the nature of C. elegans samples. These are described in detail, together with some initial qualitative and quantitative results obtained by them. The feasibility of the presented method is supported by data which show that in continuously fed worms the autophagic compartment is in the lower range of the 10(-2)% order of magnitude of the cytoplasmic volume, while immediately after molting or upon starvation in the second larval period, usually more than a 10-fold increase can be measured. In dauer larvae, individual variation of the autophagic compartment is very high. The predauer stage in daf-2 mutants doe…

Overview

Integrity signals

Registry

Crossref · 10.1016/s0076-6879(08)03228-x

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 3 references

Research works printed but not linked: 26

  1. Arstila, 1972, Studies on cellular autophagocytosis. A histochemical study on sequential alterations of mitochondria in the glucagon‐induced autophagic vacuoles of rat liver, Lab. Invest., 27, 317
  2. Ashford, 1962, Cytoplasmic components in hepatic cell lysosomes, J. Cell Biol., 12, 198, 10.1083/jcb.12.1.198 · DOI
  3. Brenner, 1974, The genetics of Caenorhabditis elegans, Genetics, 77, 71, 10.1093/genetics/77.1.71 · DOI
  4. Cao, 2007, Physiological functions of Atg6/Beclin 1: A unique autophagy‐related protein, Cell Res., 17, 839, 10.1038/cr.2007.78 · DOI · Paper page
  5. Cheong, 2008, The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae, Mol. Biol. Cell, 19, 668, 10.1091/mbc.E07-08-0826 · DOI · Paper page
  6. Clark, 1957, Cellular differentiation in the kidneys of newborn mice studied with the electron microscope, J. Biophys. Biochem. Cytol., 3, 349, 10.1083/jcb.3.3.349 · DOI
  7. de Duve, 1966, Functions of lysosomes, Annu. Rev. Physiol., 28, 435, 10.1146/annurev.ph.28.030166.002251 · DOI · Paper page
  8. Ericsson, 1969, Mechanism of cellular autophagy, Vol. 2, 345
  9. Ericsson, 1969, Studies on induced cellular autophagy. II. Characterization of the membranes bordering autophagosomes in parenchymal liver cells, Exp. Cell Res., 56, 393, 10.1016/0014-4827(69)90030-5 · DOI
  10. Glinsmann, 1966, Observations on the subcellular organization of hepatic parenchymal cells. II. Evolution of reversible alterations induced by hypoxia, Lab. Invest., 15, 762
  11. Hall, 1995, Electron microscopy and three‐dimensional image reconstruction, Methods Cell. Biol., 48, 395, 10.1016/S0091-679X(08)61397-7 · DOI
  12. Klionsky, 2008, Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes, Autophagy, 4, 1, 10.4161/auto.5338 · DOI · Paper page
  13. Kovács, 2007, Sequestration revisited: Integrating traditional electron microscopy, de novo assembly and new results, Autophagy, 3, 655, 10.4161/auto.4590 · DOI
  14. Liang, 1998, Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl‐2‐interacting protein, J. Virology, 72, 8586, 10.1128/JVI.72.11.8586-8596.1998 · DOI
  15. Meléndez, 2003, Autophagy genes are essential for dauer development and life‐span extension in C. elegans, Science, 301, 1387, 10.1126/science.1087782 · DOI · Paper page
  16. Novikoff, 1959, The proximal tubule cell in experimental hydronephrosis, J. Biophys. Biochem. Cytol., 6, 136, 10.1083/jcb.6.1.136 · DOI
  17. Ogura, 2006, The autophagy‐related kinase UNC‐51 and its binding partner UNC‐14 regulate the subcellular localization of the Netrin receptor UNC‐5 in Caenorhabditis elegans, Development, 133, 3441, 10.1242/dev.02503 · DOI
  18. Ogura, 1994, Caenorhabditis elegans unc‐51 gene required for axonal elongation encodes a novel serine/threonine kinase, Genes Dev., 8, 2389, 10.1101/gad.8.20.2389 · DOI
  19. Papadopoulos, 1986, Regression of rat liver autophagic vacuoles by locally applied cycloheximide, Lab. Invest., 54, 100
  20. Pfeifer, 1976, Lysosomen und Autophagie, Verh. Dtsch. Ges. Pathol., 60, 28
  21. Reynolds, 1963, The use of lead citrate at high pH as an electron‐opaque stain in electron microscopy, J. Cell Biol., 17, 208, 10.1083/jcb.17.1.208 · DOI
  22. Rhodin, 1954, Correlation of ultrastructural organization and function in normal and experimentally changed proximal tubule cell of the mouse kidney, Aktiebolaget Godvil, Stockholm, 1
  23. Roggo, 2002, Membrane transport in Caenorhabditis elegans: An essential role for VPS34 at the nuclear membrane, Embo J., 21, 1673, 10.1093/emboj/21.7.1673 · DOI
  24. Stack, 1994, Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol‐specific PI 3‐kinase activities, J. Biol. Chem., 269, 31552, 10.1016/S0021-9258(18)31729-0 · DOI
  25. Suzuki, 2007, Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae, FEBS Lett., 581, 2156, 10.1016/j.febslet.2007.01.096 · DOI
  26. Takács‐Vellai, 2005, Inactivation of the autophagy gene bec‐1 triggers apoptotic cell death in C. elegans, Curr. Biol., 15, 1513, 10.1016/j.cub.2005.07.035 · DOI

Cited by

Sorted by citations

Metadata

Authors
Sigmond, T., Fehér, J., Baksa, A., Pásti, G., Pálfia, Z., Takács‐Vellai, K., Kovács, J., Vellai, T., & Kovács, A. L.
Venue
Methods in Enzymology
Publisher
Elsevier
Published
2008
Pages
467-491
ISSN
0076-6879
DOI
10.1016/s0076-6879(08)03228-x
Cited by
25
References
3
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review