The DOI matches its Crossref record; 81 of the 81 references it cites were found in a registry or an index.
Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria
Open accessAbstract
Mouse kif5B gene was disrupted by homologous recombination. kif5B-/- mice were embryonic lethal with a severe growth retardation at 9.5-11.5 days postcoitum. To analyze the significance of this conventional kinesin heavy chain in organelle transport, we studied the distribution of major organelles in the extraembryonic cells. The null mutant cells impaired lysosomal dispersion, while brefeldin A could normally induce the breakdown of their Golgi apparatus. More prominently, their mitochondria abnormally clustered in the perinuclear region. This mitochondrial phenotype was reversed by an exogenous expression of KIF5B, and a subcellular fractionation revealed that KIF5B is associated with mitochondria. These data collectively indicate that kinesin is essential for mitochondrial and lysosomal dispersion rather than for the Golgi-to-ER traffic in these cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Gene targeting of the ubiquitous KIF5B in mice, however, resulted in embryonic lethality (Tanaka et al. 1998).”
From Defective kinesin heavy chain behavior in mouse kinesin light chain mutants (1999) · cites this paper as Tanaka et al. 1998
In the citing paper
“Kinesin-1C mRNA expression was upregulated in Kinesin-1B null extraembryonic membranes”
From Stable kinesin and dynein assemblies drive the axonal transport of mammalian prion protein vesicles (2011) · cites this paper as Tanaka et al., 1998
Citations
Cites
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
Kozak, M. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.20.8125 · Cited by 5,090
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993
Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport
Hirokawa, N. (1998) · Science · doi:10.1126/science.279.5350.519 · Cited by 1,689
Self-citationCharacterization of a cis-Golgi matrix protein, GM130.
Nakamura, N., Rabouille, C., Watson, R., Nilsson, T., Hui, N., Slusarewicz, P., Kreis, T. E., & Warren, W. G. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.6.1715 · Cited by 818
Altered microtubule organization in small-calibre axons of mice lacking tau protein
Harada, A., Oguchi, K., Okabe, S., Kuno, J., Terada, S., Ohshima, T., Sato-Yoshitake, R., Takei, Y., Noda, T., & Hirokawa, N. (1994) · Nature · doi:10.1038/369488a0 · Cited by 802
Self-citationOkada, Y., Yamazaki, H., Sekine‐Aizawa, Y., & Hirokawa, N. (1995) · Cell · doi:10.1016/0092-8674(95)90538-3 · Cited by 679
Self-citationMicrotubules and the endoplasmic reticulum are highly interdependent structures.
Terasaki, M. R., Chen, L. B., & Fujiwara, K. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.103.4.1557 · Cited by 650
A novel brain ATPase with properties expected for the fast axonal transport motor
Brady, S. T. (1985) · Nature · doi:10.1038/317073a0 · Cited by 618
KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria
Nangaku, M., Sato-Yoshitake, R., Okada, Y., Noda, Y., Takemura, R., Yamazaki, H., & Hirokawa, N. (1994) · Cell · doi:10.1016/0092-8674(94)90012-4 · Cited by 608
Self-citationCole, N. B., Sciaky, N., Marotta, A., Song, J. L., & Lippincott‐Schwartz, J. (1996) · Molecular Biology of the Cell · doi:10.1091/mbc.7.4.631 · Cited by 502
Hirokawa, N., Pfister, K. K., Yorifuji, H., Wagner, M. C., Brady, S. T., & Bloom, G. S. (1989) · Cell · doi:10.1016/0092-8674(89)90691-0 · Cited by 477
Self-citationChanges in lysosome shape and distribution correlated with changes in cytoplasmic pH
Heuser, J. (1989) · The Journal of cell biology · doi:10.1083/jcb.108.3.855 · Cited by 390
Hurd, D. D., & Saxton, W. M. (1996) · Genetics · doi:10.1093/genetics/144.3.1075 · Cited by 379
The role of exon sequences in splice site selection.Preprint
Watakabe, A., Tanaka, K., & Shimura, Y. (1993) · Genes & Development · doi:10.1101/gad.7.3.407 · Cited by 374
Kinesin family in murine central nervous system.
Aizawa, H., Sekine, Y., Takemura, R., Zhang, Z., Nangaku, M., & Hirokawa, N. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.119.5.1287 · Cited by 321
Self-citationRadial extension of macrophage tubular lysosomes supported by kinesin
Hollenbeck, P. J., & Swanson, J. A. (1990) · Nature · doi:10.1038/346864a0 · Cited by 295
Yamazaki, H., Nakata, T., Okada, Y., & Hirokawa, N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.130.6.1387 · Cited by 293
Self-citationKinesin associates with anterogradely transported membranous organelles in vivo.
Hirokawa, N., Sato-Yoshitake, R., Kobayashi, N., Pfister, K. K., Bloom, G. S., & Brady, S. T. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.114.2.295 · Cited by 260
Self-citationLippincott‐Schwartz, J. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.3.293 · Cited by 237
KIF3A is a new microtubule-based anterograde motor in the nerve axon.
Kondo, S., Sato-Yoshitake, R., Noda, Y., Aizawa, H., Nakata, T., Matsuura, Y., & Hirokawa, N. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.5.1095 · Cited by 237
Self-citationNakata, T., & Hirokawa, N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.4.1039 · Cited by 236
Self-citationIdentification of globular mechanochemical heads of kinesin
Scholey, J. M., Heuser, J., Yang, J. T., & Goldstein, L. S. B. (1989) · Nature · doi:10.1038/338355a0 · Cited by 228
A Novel Negative Selection for Homologous Recombinants Using Diphtheria Toxin A Fragment Gene
Yagi, T., Nada, S., Watanabe, N., Tamemoto, H., Kohmura, N., Ikawa, Y., & Aizawa, S. (1993) · Analytical Biochemistry · doi:10.1006/abio.1993.1459 · Cited by 227
Tsukamoto, T., Yokota, S., & Fujiki, Y. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.110.3.651 · Cited by 212
Saxton, W. M., Hicks, J., Goldstein, L. S. B., & Raff, E. C. (1991) · Cell · doi:10.1016/0092-8674(91)90264-y · Cited by 201
Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells.
Fath, K. R., Trimbur, G. M., & Burgess, D. R. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.126.3.661 · Cited by 201
Navone, F., Niclas, J., Hom-Booher, N., Sparks, L. L., Bernstein, H., McCaffrey, G., & Vale, R. D. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.117.6.1263 · Cited by 194
WITH APOLOGIES TO SCHEHERAZADE: TAILS OF 1001 KINESIN MOTORS
Goldstein, L. S. B. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.001535 · Cited by 181
Sekine, Y., Okada, Y., Noda, Y., Kondo, S., Aizawa, H., Takemura, R., & Hirokawa, N. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.127.1.187 · Cited by 171
Self-citationSaito, N., Okada, Y., Noda, Y., Kinoshita, Y., Kondo, S., & Hirokawa, N. (1997) · Neuron · doi:10.1016/s0896-6273(00)81243-x · Cited by 168
Self-citationTakei, Y., Harada, A., Takeda, S., Kobayashi, K., Terada, S., Noda, T., Takahashi, T., & Hirokawa, N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.6.1789 · Cited by 167
Self-citationNoda, Y., Sato-Yoshitake, R., Kondo, S., Nangaku, M., & Hirokawa, N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.129.1.157 · Cited by 167
Self-citationRodionov, V., Gyoeva, F. K., Tanaka, E. M., Bershadsky, A. D., Vasiliev, J. M., & Gelfand, V. I. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.123.6.1811 · Cited by 156
Cloning and localization of a conventional kinesin motor expressed exclusively in neurons
Niclas, J., Navone, F., Hom-Booker, N., & Vale, R. D. (1994) · Neuron · doi:10.1016/0896-6273(94)90314-x · Cited by 153
Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome
Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., Kanai, Y., & Hirokawa, N. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.18.9654 · Cited by 153
Self-citationHost cell invasion by trypanosomes requires lysosomes and microtubule/kinesin-mediated transport.
Rodrı́guez, A., Samoff, E., Rioult, M. G., Chung, A., & Andrews, N. W. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.134.2.349 · Cited by 153
Kinesin-mediated organelle translocation revealed by specific cellular manipulations.
Feiguin, F. M., Ferreira, A., Kosik, K. S., & Cáceres, A. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.127.4.1021 · Cited by 151
Actin dynamics in growth cones
Okabe, S., & Hirokawa, N. (1991) · Journal of Neuroscience · doi:10.1523/jneurosci.11-07-01918.1991 · Cited by 146
Self-citationJasmin, B. J., Cartaud, J., Bornens, M., & Changeux, J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.18.7218 · Cited by 141
Association of kinesin with characterized membrane‐bounded organelles
Leopold, P. L., McDowall, A. W., Pfister, K. K., Bloom, G. S., & Brady, S. T. (1992) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.970230104 · Cited by 137
Organelle transport along microtubules — the role of KIFs
Hirokawa, N. (1996) · Trends in Cell Biology · doi:10.1016/0962-8924(96)10003-9 · Cited by 131
Self-citationGudkov, A. V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A., & Roninson, I. B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.9.3744 · Cited by 122
Pereira, A., Dalby, B., Stewart, R. J., Doxsey, S., & Goldstein, L. S. B. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.5.1081 · Cited by 121
Effects of kinesin mutations on neuronal functions
Gho, M., McDonald, K., Ganetzky, B., & Saxton, W. M. (1992) · Science · doi:10.1126/science.1384131 · Cited by 120
Elluru, R. G., Bloom, G. S., & Brady, S. T. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.1.21 · Cited by 118
Identification and molecular evolution of new dynein-like protein sequences in rat brain
Tanaka, Y., Zhang, Z., & Hirokawa, N. (1995) · Journal of Cell Science · doi:10.1242/jcs.108.5.1883 · Cited by 115
Self-citationWright, B. D. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.113.4.817 · Cited by 111
Lytic granules from cytotoxic T cells exhibit kinesin-dependent motility on microtubules in vitro
Burkhardt, J. K., McIIvain, J. M., Sheetz, M. P., & Argon, Y. (1993) · Journal of Cell Science · doi:10.1242/jcs.104.1.151 · Cited by 103
Yokoi, H., Natsuyama, S., Iwai, M., Noda, Y., Mori, T., Mori, K. J., Fujita, K., Nakayama, H., & Fujita, J. (1993) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1993.2112 · Cited by 103
Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain
Patel, N., Thierry-Mieg, D., & Mancillas, J. R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.19.9181 · Cited by 96
Axonal transport and the cytoskeleton
Hirokawa, N. (1993) · Current Opinion in Neurobiology · doi:10.1016/0959-4388(93)90144-n · Cited by 96
Self-citationIsolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.
Yang, J. T., Saxton, W. M., & Goldstein, L. S. B. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.6.1864 · Cited by 93
The primary structure and analysis of the squid kinesin heavy chain.
Kosik, K. S., Orecchio, L. D., Schnapp, B. J., Inouye, H., & Neve, R. L. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39764-9 · Cited by 92
A Specific DNA Sequence Controls Termination of Transcription in the Gastrin Gene
Sato, K., Ito, R., Baek, K., & Agarwal, K. L. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.4.1032 · Cited by 87
A Collection of cDNA Clones with Specific Expression Patterns in Mouse Brain
Kato, K. (1990) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1990.tb00460.x · Cited by 84
Association of kinesin with the Golgi apparatus in rat hepatocytes
Marks, D. L., Larkin, J. M., & McNiven, M. A. (1994) · Journal of Cell Science · doi:10.1242/jcs.107.9.2417 · Cited by 84
Morfini, G. A., Quiroga, S., Rosa, A. L., Kosik, K. S., & Cáceres, A. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.3.657 · Cited by 78
Henson, J. H., Nesbitt, D. J., Wright, B. D., & Scholey, J. M. (1992) · Journal of Cell Science · doi:10.1242/jcs.103.2.309 · Cited by 78
Wright, B. D., Terasaki, M., & Scholey, J. M. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.123.3.681 · Cited by 77
Okada, Y., Sato-Yoshitake, R., & Hirokawa, N. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-04-03053.1995 · Cited by 72
Self-citationMotoring to the Finish: Kinesin and Dynein Work Together to Orient the Yeast Mitotic Spindle
Stearns, T. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.5.957 · Cited by 70
Amaratunga, A., Morin, P. J., Kosik, K. S., & Fine, R. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85352-8 · Cited by 67
Hurd, D. D., Stern, M., & Saxton, W. M. (1996) · Genetics · doi:10.1093/genetics/142.1.195 · Cited by 57
Meng, Y. X., Wilson, G., Avery, M. C., Varden, C. E., & Balczon, R. (1997) · Endocrinology · doi:10.1210/endo.138.5.5139 · Cited by 53
Tanaka, Y., Kawahata, K., Nakata, T., & Hirokawa, N. (1992) · Brain Research · doi:10.1016/0006-8993(92)91557-u · Cited by 52
Self-citationHamm‐Alvarez, S. F., da Costa, S. R., YANG, T., Wei, X., Gierow, P., & Mircheff, A. K. (1997) · Experimental Eye Research · doi:10.1006/exer.1996.0198 · Cited by 47
A Specific DNA Sequence Controls Termination of Transcription in the Gastrin Gene
Sato, K., Ito, R., Baek, K., et al. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.4.1032-1043.1986 · Cited by 46
Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria
Jellali, A., Metz‐Boutigue, M., Surgucheva, I. G., Jancsik, V., Schwartz, C., Filliol, D., Gelfand, V. I., & Rendón, Á. (1994) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.970280108 · Cited by 43
Hirokawa, N. (1996) · Cell Structure and Function · doi:10.1247/csf.21.357 · Cited by 40
Self-citationFunakoshi, T., Takeda, S., & Hirokawa, N. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.133.6.1347 · Cited by 38
Self-citationIdentification, Partial Characterization, and Genetic Mapping of Kinesin-like Protein Genes in Mouse
Yang, Z., Hanlon, D., Marszalek, J. R., & Goldstein, L. S. B. (1997) · Genomics · doi:10.1006/geno.1997.4901 · Cited by 36
Kinesin localizes to the trans-Golgi network regardless of microtubule organization.
Johnson, K. J., Hall, E. S., & Boekelheide, K. (1996) · PubMed · Cited by 34
Routine assessment of morphology and growth: scoring syste1ns and measurements of size
Brown, N. A. (1990) · doi:10.1093/oso/9780199630882.003.0006 · Cited by 30
Intracellular distribution of kinesin in chromaffin cells.
Schmitz, F., Wallis, K. T., Rho, M., Drenckhahn, D., & Db, M. (1994) · PubMed · Cited by 28
Vignali, G., Niclas, J., Sprocati, M. T., Vale, R. D., Sirtori, C. R., & Navone, F. (1996) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1996.tb01238.x · Cited by 27
Lin, S. X. H., Pfister, K. K., & Collins, C. A. (1996) · Cell Motility and the Cytoskeleton · doi:10.1002/(sici)1097-0169(1996)34:4 · Cited by 22
BOST-USINGER, L., CHEN, R. J., HILLMAN, D., Park, H., & Burnside, B. (1997) · Experimental Eye Research · doi:10.1006/exer.1996.0271 · Cited by 18
Rat pancreas kinesin: identification and potential binding to microtubules.
Malekzadeh-Hemmat, K., Gendry, P., & Launay, J. F. (1993) · PubMed · Cited by 12
Noda, Y., Sato-Yoshitake, R., Kondo, S., Nangaku, M., & Hirokawa, N. (1994) · Neuroscience Research Supplements · doi:10.1016/0921-8696(94)92499-6 · Cited by 6
Self-citationStürmer, K., & Baumann, O. (1996) · Cell and Tissue Research · doi:10.1007/s004410050725 · Cited by 4
Microinjection of RNA and DNA into Somatic Cells
Graessmann, M., & Graessmann, A. (1994) · Cell Biology · doi:10.1016/b978-0-12-164717-9.50005-2 · Cited by 2
Research works printed but not linked: 1
- Malekzadeh-Hemmat, 1993, Rat pancreas kinesin, Cell. Mol. Biol., 39, 279
Cited by
Sorted by citations
Chen, H., Detmer, S. A., Ewald, A. J., Griffin, E. E., Fraser, S. E., & Chan, D. C. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200211046 · Cited by 2,601
Kinesin superfamily motor proteins and intracellular transport
Hirokawa, N., Noda, Y., Tanaka, Y., & Niwa, S. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2774 · Cited by 1,859
Self-citationDynamin-related Protein Drp1 Is Required for Mitochondrial Division in Mammalian Cells
Smirnova, E., Griparić, L., Shurland, D., & van der Bliek, A. M. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.8.2245 · Cited by 1,938
Nonaka, S., Tanaka, Y., Okada, Y., Takeda, S., Harada, A., Kanai, Y., Kido, M. A., & Hirokawa, N. (1998) · Cell · doi:10.1016/s0092-8674(00)81705-5 · Cited by 1,639
Self-citationThe axonal transport of mitochondria
Saxton, W. M., & Hollenbeck, P. J. (2012) · Journal of Cell Science · doi:10.1242/jcs.053850 · Cited by 1,218
Metadata
- Authors
- Tanaka, Y., Kanai, Y., Okada, Y., Nonaka, S., Takeda, S., Harada, A., & Hirokawa, N.
- Affiliations
- The University of Tokyo (JP)
- Corresponding author
- Hirokawa, Nobutaka
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1998-06
- Volume
- 93
- Issue
- 7
- Pages
- 1147-1158
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2095841601
- Semantic Scholar
- 936e428e5d9d833db465aefd6f33c879e6ea0468
- Cited by
- 654
- References
- 81
- Citations by year
- 2026: 12 · 2025: 13 · 2024: 24 · 2023: 13 · 2022: 24 · 2021: 27 · 2020: 25 · 2019: 23 · 2018: 24 · 2017: 27 · 2016: 34 · 2015: 24 · 2014: 22 · 2013: 27 · 2012: 31
- FWCI
- 9.24
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- KIF5B, mitochondrial clustering, organelle transport, Golgi apparatus, brefeldin A, extraembryonic cells, embryonic lethality
- MeSH terms
- Animals, Anti-Bacterial Agents, Cell Fractionation, Cell Nucleus, Cells, Cultured, Cyclopentanes, Genes, Lethal, Golgi Apparatus, Lysosomes, Microtubules, Mitochondria, Phenotype, RNA, Messenger, Yolk Sac, Nocodazole, Kinesins, Mice, Knockout, Gene Expression Regulation, Developmental, Macrolides, Brefeldin A, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
