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The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/W mice

Dick, John E., Magli, Maria Cristina, Huszar, Dennis, Phillips, Robert A., Bernstein, Alan·1985·Cell
DOI 10.1016/s0092-8674(85)80102-1
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Abstract

We have used the random chromosomal integration sites of retrovirus vectors as unique clonal markers to analyze cell lineage relationships within the hemopoietic stem cell hierarchy. Using a high efficiency protocol for retrovirus-mediated gene transfer, anemic W/Wv mutant mice were reconstituted with bone marrow cells infected with a NEO vector. Analysis of the DNA from bone marrow, thymus, and spleen of these reconstituted W/Wv mice indicated insertion of the vector into primitive pluripotent stem cells capable of producing both myeloid and lymphoid progeny as well as into more committed stem cells apparently restricted to either the myeloid or lymphoid lineages. The neo gene was also expressed in these mice, as they contained a variety of G418 resistant in vitro colony-forming cells. These results demonstrate high-efficiency gene transfer and expression in primitive hemopoietic stem cells and provide a direct approach for analyzing the hemopoietic stem cell hierarchy.

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Research works printed but not linked: 26

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Cited by

Metadata

Authors
Dick, J. E., Magli, M. C., Huszar, D., Phillips, R. A., & Bernstein, A.
Venue
Cell
Publisher
Elsevier BV
Published
1985-08
Volume
42
Issue
1
Pages
71-79
ISSN
0092-8674
DOI
10.1016/s0092-8674(85)80102-1
Cited by
446
References
1
Language
en
Licence

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