Presence of fetal DNA in maternal plasma and serum
Abstract
Background The potential use of plasma and serum for molecular diagnosis has generated interest. Tumour DNA has been found in 'the plasma and serum of cancer patients, and molecular analysis has been done on this material. We investigated the equivalent condition in pregnancy-that is, whether fetal DNA is present in maternal plasma and serum.Methods We used a rapid-boiling method to extract DNA from plasma and serum. DNA from plasma, serum, and nucleated blood cells from 43 pregnant women underwent a sensitive Y-PCR assay to detect circulating male fetal DNA from women bearing male fetuses.Findings Fetus-derived Y sequences were detected in 24 (80%) of the 30 maternal plasma samples, and in 21 (70%) of the 30 maternal serum samples, from women bearing male fetuses. These results were obtained with only 10 microL of the samples. When DNA from nucleated blood cells extracted from a similar volume of blood was used, only five (17%) of the 30 samples gave a positive Y signal. None of the 13 women bearing female fetuses, and none of the ten non-pregnant control women, had positive results for plasma, serum or nucleated blood cells.Interpretation Our finding of circulating fetal DNA in maternal plasma may have implications for non-invasive prenatal diagnosis, and for improving our understanding of the fetomaternal relationship.
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- Authors
- Lo, Y. M. D., Corbetta, N., Chamberlain, P. F., Rai, V., Sargent, I. L., Redman, C. W., & Wainscoat, J. S.
- Affiliations
- John Radcliffe Hospital (GB)
- Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico (IT)
- University of Oxford (GB)
- Venue
- The Lancet
- Publisher
- Elsevier BV
- Published
- 1997-08
- Volume
- 350
- Issue
- 9076
- Pages
- 485-487
- ISSN
- 0099-5355 (ISSN-L)
- 0140-6736
- OpenAlex
- W2100977485
- Cited by
- 3,129
- References
- 17
- Citations by year
- 2026: 95 · 2025: 128 · 2024: 141 · 2023: 160 · 2022: 155 · 2021: 176 · 2020: 171 · 2019: 163 · 2018: 191 · 2017: 137 · 2016: 168 · 2015: 179 · 2014: 173 · 2013: 172 · 2012: 138
- FWCI
- 31.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Lancet (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Screening and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Health Sciences › Medicine › Obstetrics and Gynecology › Maternal and Perinatal Health Interventions
- Keywords
- fetal DNA, maternal plasma, maternal serum, noninvasive prenatal diagnosis, fetal sex determination, pregnancy
- MeSH terms
- DNA, Female, Fetal Proteins, Humans, Male, Plasma, Pregnancy, Prenatal Diagnosis, Sensitivity and Specificity, Y Chromosome, Reproducibility of Results, Case-Control Studies, Polymerase Chain Reaction
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