In vivo protein expression from mRNA delivered into adult rat brain

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,459 · see citing works

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Chomczyński, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 47,092 · see citing works

Direct Gene Transfer into Mouse Muscle in Vivo

Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173 · see citing works

Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo.

Boczkowski, D., Nair, S. K., Snyder, D., & Gilboa, E. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.465 · Cited by 915 · see citing works

Cationic liposome-mediated RNA transfection.

Malone, R. W., Felgner, P. L., & Verma, I. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6077 · Cited by 911 · see citing works

The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency.

Gallie, D. R. (1991) · Genes & Development · doi:10.1101/gad.5.11.2108 · Cited by 868 · see citing works

Induction of virus‐specific cytotoxic T lymphocytes <i>in vivo</i> by liposome‐entrapped mRNA

Martinon, F., Krishnan, S., Lenzen, G., Magné, R., Gomard, E., Guillet, J., Lévy, J., & Meulien, P. (1993) · European Journal of Immunology · doi:10.1002/eji.1830230749 · Cited by 473 · see citing works

Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA

Nair, S. K., Boczkowski, D., Morse, M., Cumming, R. I., Lyerly, H. K., & Gilboa, E. (1998) · Nature Biotechnology · doi:10.1038/nbt0498-364 · Cited by 398 · see citing works

Integrin binding and mechanical tension induce movement of mRNA and ribosomes to focal adhesions

Chicurel, M., Singer, R. H., Meyer, C. J., & Ingber, D. E. (1998) · Nature · doi:10.1038/33719 · Cited by 374 · see citing works

Untitled work

(1995) · PubMed · Cited by 330 · see citing works

Reversal of Diabetes Insipidus in Brattleboro Rats: Intrahypothalamic Injection of Vasopressin mRNA

Jirikowski, G. F., Sanna, P. P., Maciejewski-Lenoir, D., & Bloom, F. E. (1992) · Science · doi:10.1126/science.1546298 · Cited by 282 · see citing works

Lipofection of cDNAs in the embryonic vertebrate central nervous system

Holt, C. E., Garlick, N., & Cornel, E. (1990) · Neuron · doi:10.1016/0896-6273(90)90095-w · Cited by 260 · see citing works

Molecular Characterization of the Dendritic Growth Cone: Regulated mRNA Transport and Local Protein Synthesis

Crino, P. B., & Eberwine, J. H. (1996) · Neuron · doi:10.1016/s0896-6273(00)80248-2 · Cited by 257 · see citing works

The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs inXenopusoocytes

Drummond, D. R., Armstrong, J., & Colman, A. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.20.7375 · Cited by 235 · see citing works

Translational Efficiency Is Regulated by the Length of the 3′ Untranslated Region

Tanguay, R. L., & Gallie, D. R. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.1.146 · Cited by 204 · see citing works

HIV Gag mRNA Transfection of Dendritic Cells (DC) Delivers Encoded Antigen to MHC Class I and II Molecules, Causes DC Maturation, and Induces a Potent Human In Vitro Primary Immune Response

Weissman, D., Ni, H., Scales, D. A., Dude, A., Capodici, J., McGibney, K., Abdool, A. J., Isaacs, S. N., Cannon, G., & Karikó, K. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.8.4710 · Cited by 178 · see citing works

Regional Expression of Heat Shock Protein-70 mRNA and c-Fos mRNA following Focal Ischemia in Rat Brain

Welsh, F. A., Moyer, D. J., & Harris, V. A. (1992) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.1992.30 · Cited by 168 · see citing works

Possible Involvement of poly(A) in protein synthesks

Jacobson, A., & Favreau, M. R. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.18.6353 · Cited by 165 · see citing works

Axonal transport of neuropeptide encoding mRNAs within the hypothalamo‐hypophyseal tract of rats.

Mohr, E., Fehr, S., & Richter, D. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb07781.x · Cited by 165 · see citing works

Gene gun delivery of mRNA in situ results in efficient transgene expression and genetic immunization.

Qiu, P., Ziegelhoffer, P. R., Sun, J., & Yang, N. S. (1996) · PubMed · Cited by 150 · see citing works

RNA Melanoma Vaccine: Induction of Antitumor Immunity by Human Glycoprotein 100 mRNA Immunization

Zhou, W.-Z., Hoon, D. S. �., Huang, S., Fujii, S., Hashimoto, K., Morishita, R., & Kaneda, Y. (1999) · Human Gene Therapy · doi:10.1089/10430349950016762 · Cited by 135 · see citing works

The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation

Gallie, D., Tanguay, R. L., & Leathers, V. L. (1995) · Gene · doi:10.1016/0378-1119(95)00521-7 · Cited by 125 · see citing works

Aggregation of vasopressin mRNA in a subset of axonal swellings of the median eminence and posterior pituitary: light and electron microscopic evidence

Trembleau, A., Morales, M., & Bloom, F. E. (1994) · Journal of Neuroscience · doi:10.1523/jneurosci.14-01-00039.1994 · Cited by 104 · see citing works

A sindbis virus mRNA polynucleotide vector achieves prolonged and high level heterologous gene expression<i>in vivo</i>

Johanning, F. W., Conry, R. M., LoBuglio, A. F., Wright, M., Sumerel, L. A., Pike, M. J., & Curiel, D. T. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.9.1495 · Cited by 99 · see citing works

The histone 3'-terminal stem-loop is necessary for translation in Chinese hamster ovary cells

Gallie, D. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.10.1954 · Cited by 95 · see citing works

Optimization of methods to achieve mRNA-mediated transfection of tumor cells in vitro and in vivo employing cationic liposome vectors.

Lu, D., Benjamin, R., Kim, M., Conry, R. M., & Curiel, D. (1994) · PubMed · Cited by 89 · see citing works

Overexpression of urokinase receptor in mammalian cells following administration of the in vitro transcribed encoding mRNA

Karikó, K., Kuo, A., & Barnathan, E. S. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300930 · Cited by 89 · see citing works

Applications of gene therapy to the CNS

Blömer, U., Naldini, L., Verma, I. M., Trono, D., & Gage, F. H. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.supplement_1.1397 · Cited by 88 · see citing works

The principles of gene therapy for the nervous system

Karpati, G., Lochmüller, H., Nalbantoglu, J. N., & Durham, H. D. (1996) · Trends in Neurosciences · doi:10.1016/0166-2236(96)89620-2 · Cited by 88 · see citing works

RNA in the axonal domain: a new dimension in neuronal functioning?

van Minnen, J. (1994) · The Histochemical Journal · doi:10.1007/bf00160050 · Cited by 64 · see citing works

Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines

Miyagi, Y., Sugiyama, A., Asai, A., Okazaki, T., Kuchino, Y., & Kerr, S. J. (1995) · Cancer Letters · doi:10.1016/0304-3835(95)03737-h · Cited by 62 · see citing works

Liposome-mediated NGF gene transfection following neuronal injury: potential therapeutic applications

Zou, L., Huang, L., Hayes, R. L., Black, C., Qiu, Y. H., Perez-Polo, J. R., Le, W., Clifton, G. L., & Yang, K. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300936 · Cited by 59 · see citing works

Sodium phosphate enhances plasmid DNA expression in vivo

Hartikka, J., Bozoukova, V., Jones, D., Mahajan, R., Wloch, M. K., Sawdey, M., Buchner, C., Sukhu, L., Barnhart, K. M., Abai, A. M., et al. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301226 · Cited by 52 · see citing works

Phosphate-enhanced transfection of cationic lipid-complexed mRNA and plasmid DNA

Karikó, K., Kuo, A., Barnathan, E. S., & Langer, D. J. (1998) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(97)00238-1 · Cited by 48 · see citing works

Defective viral vectors as agents for gene transfer in the nervous system

Kaplitt, M. G., & Makimura, H. (1997) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(96)00132-x · Cited by 43 · see citing works

Gene Expression along the Cerebral-Spinal Axis after Regional Gene Delivery

Meuli‐Simmen, C., Liu, Y., Yeo, T. T. S., Liggitt, D., Tu, G., Yang, T., Meuli, M., Knauer, S., Heath, T. D., Longo, F. M., et al. (1999) · Human Gene Therapy · doi:10.1089/10430349950016735 · Cited by 36 · see citing works

Lipofectin‐aided cell delivery of ribozyme targeted to human urokinase receptor mRNA

Karikó, K., Megyeri, K., Xiao, Q., & Barnathan, E. S. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)00914-7 · Cited by 35 · see citing works

[43] Cationic liposome-mediated RNA transfection

Dwarki, V., Malone, R. W., & Verma, I. M. (1993) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(93)17093-k · Cited by 34 · see citing works

Gene Therapy for Cerebrovascular Disease

Weihl, C., Macdonald, R. L., Stoodley, M. A., Lüders, J. C., & Lin, G. (1999) · Neurosurgery · doi:10.1097/00006123-199902000-00001 · Cited by 30 · see citing works

Reduction of exogenous vasopressin RNA poly(A) tail length increases its effectiveness in transiently correcting diabetes insipidus in the Brattleboro rat.

Maciejewski-Lenoir, D., Jirikowski, G. F., Sanna, P. P., & Bloom, F. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.4.1435 · Cited by 29 · see citing works

Incorporation of β-globin untranslated regions into a Sindbis virus vector for augmentation of heterologous mRNA expression

Strong, T. V., Hampton, T., Louro, I. D., Bilbao, G., Conry, R. M., & Curiel, D. (1997) · Gene Therapy · doi:10.1038/sj.gt.3300423 · Cited by 26 · see citing works

Influence of Capping and Polyadenylation on mRNA Expression and on Antisense RNA Mediated Inhibition of Gene Expression

Bechler, K. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7789 · Cited by 9 · see citing works

Increased Vasopressin Secretion from Hypothalamic Cultures Following Administration of Exogenous Vasopressin mRNA

Mathiasen, J. R., Maciejewski-Lenoir, D., Bloom, F. E., & Sladek, C. D. (1996) · Experimental Neurology · doi:10.1006/exnr.1996.0150 · Cited by 3 · see citing works

Lipid-mediated delivery of RNA is more efficient than delivery of DNA in non-dividing cells

Zou, S., Scarfo, K. A., Nantz, M. H., & Hecker, J. G. (2010) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2010.01.019 · Cited by 161 · see citing works

mRNA therapeutics deliver a hopeful message

Zhong, Z., Cafferty, S. M., Combes, F., Huysmans, H., Temmerman, J. D., Gitsels, A., Vanrompay, D. C. G., Catani, J. P., & Sanders, N. N. (2018) · Nano Today · doi:10.1016/j.nantod.2018.10.005 · Cited by 124 · see citing works

Mechanistic Studies of an Automated Lipid Nanoparticle Reveal Critical Pharmaceutical Properties Associated with Enhanced mRNA Functional Delivery In Vitro and In Vivo

Cui, L., Hunter, M. R., Sonzini, S., Pereira, S. G. T., Romanelli, S. M., Liu, K., Li, W., Liang, L., Yang, B., Mahmoudi, N., et al. (2021) · Small · doi:10.1002/smll.202105832 · Cited by 78 · see citing works

Recombinant messenger RNA technology and its application in cancer immunotherapy, transcript replacement therapies, pluripotent stem cell induction, and beyond

Vallazza, B., Petri, S., Poleganov, M. A., Eberle, F., Kuhn, A. N., & Şahin, U. (2015) · Wiley Interdisciplinary Reviews - RNA · doi:10.1002/wrna.1288 · Cited by 73 · see citing works

Stability of mRNA/Cationic Lipid Lipoplexes in Human and Rat Cerebrospinal Fluid: Methods and Evidence for Nonviral mRNA Gene Delivery to the Central Nervous System

Anderson, D. M., Hall, L. L., Ayyalapu, A. R., Irion, V. R., Nantz, M. H., & Hecker, J. G. (2003) · Human Gene Therapy · doi:10.1089/10430340360535751 · Cited by 66 · see citing works

Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection

Tuma, J., Chen, Y.-J., Collins, M. G., Paul, A., Li, J., Han, H., Sharma, R., Murthy, N., & Lee, H. Y. (2023) · Biochemistry · doi:10.1021/acs.biochem.3c00371 · Cited by 65 · see citing works

Inhibition of Xenograft Tumor Growth by Gold Nanoparticle-DNA Oligonucleotide Conjugates-Assisted Delivery of BAX mRNA

Yeom, J., Ryou, S.-M., Won, M., Park, M., Bae, J., & Lee, K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0075369 · Cited by 51 · see citing works

Delivery of synthetic mRNAs for tissue regeneration

Steinle, H., Weber, M., Stoppelkamp, S., Große‐Berkenbusch, K., Golombek, S., Weber, M., Çanak-Ipek, T., Trenz, S.-M., Schlensak, C., & Avci‐Adali, M. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.114007 · Cited by 41 · see citing works

Lipid Nanoparticles Enhance mRNA Delivery to the Central Nervous System Upon Intrathecal Injection

Xue, Y., Wang, C., Li, H., Du, S., Zhong, Y., Zhang, Y., Wang, S., Guo, K., Hou, X., Kang, D. D., et al. (2025) · Advanced Materials · doi:10.1002/adma.202417097 · Cited by 34 · see citing works

Delivering therapeutic RNA into the brain using extracellular vesicles

Shirmast, P., Shahri, M. A., Brent, A., Idris, A., & McMillan, N. A. J. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102373 · Cited by 30 · see citing works

Non-Viral, Lipid-Mediated DNA and mRNA Gene Therapy of the Central Nervous System (CNS): Chemical-Based Transfection

Hecker, J. G. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3271-9_23 · Cited by 29 · see citing works

The legacy of mRNA engineering: A lineup of pioneers for the Nobel Prize

Janowski, M., & Andrzejewska, A. (2022) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2022.07.003 · Cited by 11 · see citing works

Prix Nobel de physiologie ou médecine 2023 : Katalin Karikó et Drew Weissman

Dieu‐Nosjean, M., & Teillaud, J. (2024) · médecine/sciences · doi:10.1051/medsci/2024002 · Cited by 5 · see citing works

Nonviral, Cationic Lipid-Mediated Delivery of mRNA

Hecker, J. G. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-260-5_5 · Cited by 5 · see citing works

Passive inhaled mRNA vaccination for SARS-Cov-2

Yeo, W. S., & Ng, Q. X. (2020) · Medical Hypotheses · doi:10.1016/j.mehy.2020.110417 · Cited by 3 · see citing works

mRNA therapeutics for central nervous system disorders

Fukushima, Y., & Itaka, K. (2022) · Drug Delivery System · doi:10.2745/dds.37.247 · Cited by 2 · see citing works

Lipid nanoparticle (LNP)-mediated cytoplasmic expression of single-stranded DNA and its application in Mpox vaccine

Zhang, K., Liu, C., Ge, R., Zhang, Z., Liu, Q., Mo, O., Li, X., Liu, X., Li, H., & Hao, P. (2026) · Journal of Controlled Release · doi:10.1016/j.jconrel.2026.114740 · Cited by 2 · see citing works

Nucleic Acid Introduction into Primary Neurons and Glia

Sul, J., Sharma, A. K., Eberwine, J. H., & Haydon, P. G. (2009) · Elsevier eBooks · doi:10.1016/b978-008045046-9.00873-1 · Cited by 1 · see citing works

Life-saving vaccines awarded

(2023) · Nature Materials · doi:10.1038/s41563-023-01770-3 · Cited by 1 · see citing works

Modified self-amplifying RNA mediates robust and prolonged gene expression in the mouse and ex vivo human brainPreprint

Freire, J., Pang, S., McGee, J. E., Heinrich, M. J., Xie, J., Hammarlund, E., Shaw, D. C., Zhou, Y., Wang, Y., Porter, C., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.30.685635 · Cited by 1 · see citing works

Bibliometric and visualized mapping: two decades of lipidomics, with special focus on pregancy and womenPreprint

Zhang, L., Zhou, Y., & Zhou, J. (2023) · medRxiv · doi:10.1101/2023.09.07.23295179

Перемога мРНК-вакцин над пандемією коронавірусу SARS-CoV-2, який викликає захворювання COVID-19, або як синтетична мРНК прослизнула повз вроджений імунний захист клітини

Комісаренко, С. В., & Романюк, С. И. (2023) · Visnik Nacional noi academii nauk Ukrai ni · doi:10.15407/visn2023.12.003

Nanoparticles in Delivery Across the Blood-Brain Barrier

Liu, J. (2025) · Theoretical and Natural Science · doi:10.54254/2753-8818/2025.au25804

Intrinsic and Extrinsic Factors for the Regeneration of Brainstem Axons after Spinal Cord Injury

Williams, R. (2010)

579. In-Vivo Time Course of Reporter Gene Expression after Nucleic Acid Delivery to the Lateral Ventricle in Rat Brain

(2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.07.119

Whole Animal In Vivo Imaging After Transient, Nonviral Gene delivery to the Rat Central Nervous System

Hauck, E. S., Zou, S., Scarfo, K. A., Nantz, M. H., & Hecker, J. G. (2008) · Molecular Therapy · doi:10.1038/mt.2008.183 · Cited by 22 · see citing works

Intranasal Delivery and Transfection of mRNA Therapeutics in the Brain Using Cationic Liposomes

Dhaliwal, H. K., Fan, Y., Kim, J., & Amiji, M. M. (2020) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.0c00170 · Cited by 136 · see citing works

Stability of mRNA/Cationic Lipid Lipoplexes in Human and Rat Cerebrospinal Fluid: Methods and Evidence for Nonviral mRNA Gene Delivery to the Central Nervous System

Anderson, D. M., Hall, L. L., Ayyalapu, A. R., Irion, V. R., Nantz, M. H., & Hecker, J. G. (2003) · Human Gene Therapy · doi:10.1089/10430340360535751 · Cited by 66 · see citing works

494. Topical Delivery of mRNA to the Murine Lung and Nasal Epithelium

(2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.06.415