A sindbis virus mRNA polynucleotide vector achieves prolonged and high level heterologous gene expression in vivo

Johanning, F. W.; Conry, R. M.; LoBuglio, A. F.; Wright, M.; Sumerel, L. A.; Pike, M. J.; Curiel, D. T.

doi:10.1093/nar/23.9.1495

Cited by 99RNA Interference and Gene DeliveryImmunotherapy and Immune ResponsesVirus-based gene therapy research

A sindbis virus mRNA polynucleotide vector achieves prolonged and high level heterologous gene expression in vivo

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Johanning, F. W., Conry, R. M., LoBuglio, A. F., Wright, M., Sumerel, L. A., Pike, M. J., Curiel, D. T.·1995·Nucleic Acids Research

DOI 10.1093/nar/23.9.1495PMC PMC306888

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Abstract

The direct intramuscular delivery of naked plasmid DNA has been demonstrated to allow expression of encoded heterologous genes in the target myocytes. The method has been employed to elicit immunization based upon delivery of antigen encoding plasmid DNA. For application in the context of achieving anti-tumor immunization against antigenic transforming oncoproteins, delivery of plasmid DNAs encoding these molecules would create significant potential safety hazards. As an alternative to DNA polynucleotide vectors, we explored the utility of mRNA vehicles for inducing foreign gene expression in muscle cells in vivo. Synthetic reporter-gene encoding mRNA transcripts were derived for this analysis. The Sindbis virus vector was also used to derive luciferase mRNA transcripts which possessed self-replication capacity. In these studies, it could be shown that the replicative vector was capable of directing significantly elevated levels of reporter gene expression in myocytes compared to a non-replicative mRNA species. In addition, the replicative species was capable of achieving significantly prolonged levels of in vivo gene expression compared to non-replicative mRNA. Both of these characteristics will make replicative mRNA vectors of utility for polynucleotide-based immunization protocols.

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Injection of naked mRNA or self-amplifying RNA in vivo induces gene expression and generates immune responses, with self-amplifying RNA being more efficient for gene expression in situ.
“Injection of naked mRNA or self-amplifying RNA in vivo induces gene expression and generates immune responses (4, 11-13), with self-amplifying RNA being more efficient for gene expression in situ (13,14).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [13]
The in vivo gene-expression results report that injected replicative mRNA produced substantially more luciferase than non-replicative mRNA in muscle. The Discussion reports an immune response from prior CEA mRNA injections, but this study did not establish that its self-amplifying RNA generated an immune response; it discusses that possibility as a hypothesis (page not provided). (LLM, conf=0.87)
Experimental — accuracy not yet measured
The shorter duration of expression in situ after RNA immunization compared with pDNA may be related to the induction of apoptosis of the transfected cell, which could occur during RNA amplification, leading to transient gene expression.
“The shorter duration of expression in situ after RNA immunization compared with pDNA may be related to the induction of apoptosis of the transfected cell, which could occur during RNA amplification, leading to transient gene expression (12,13).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [13]
The in vivo results report that replicative mRNA expression returned to baseline within 28 days, while plasmid DNA expression remained above baseline (Augmented gene expression in vivo). The Discussion proposes host-cell protein-synthesis inhibition and resulting cytopathogenicity as one possible explanation for the decline in mRNA-directed expression. It does not specifically identify apoptosis or establish that it occurs during RNA amplification. (LLM, conf=0.85)
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Authors: Johanning, F. W., Conry, R. M., LoBuglio, A. F., Wright, M., Sumerel, L. A., Pike, M. J., & Curiel, D. T.
Affiliations: University of Alabama at Birmingham (US)
Corresponding author: Curiel, D. T.
ORCID: Curiel, D. T.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 1995
Volume: 23
Issue: 9
Pages: 1495-1501
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/23.9.1495
PMC: PMC306888
OpenAlex: W2094992863
Cited by: 99
References: 25
Citations by year: 2025: 1 · 2024: 4 · 2023: 4 · 2022: 1 · 2021: 13 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 3
FWCI: 2.36
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Nucleic Acids Research (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords: Sindbis virus, heterologous expression, intramuscular administration, muscle cells, luciferase reporter
MeSH terms: Animals, Cells, Cultured, Female, Genetic Vectors, Cricetinae, Liposomes, Mice, Inbred C57BL, Plasmids, RNA, Messenger, Sindbis Virus, Gene Expression, Drug Delivery Systems, Gene Transfer Techniques, Muscle, Skeletal, Mice

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A sindbis virus mRNA polynucleotide vector achieves prolonged and high level heterologous gene expression in vivo

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