Expression of mRNA for the elav-like neural-specific RNA binding protein, HuD, during nervous system development
Abstract
The expression of mRNA for the neuronal antigen HuD (Elavl4) associated with paraneoplastic encephalomyelitis and sensory neuronopathy was evaluated in the developing and adult rat nervous system. Using RNase protection assay and non-radioactive in situ hybridization histochemistry HuD expression was shown to be expressed at high levels at the earliest time point observed (E15), but declined significantly during the first postnatal week to levels which were maintained into adulthood. In the adult, HuD expression became restricted primarily to large pyramidal-like neurons. Exceptions of note were many smaller neurons within a variety of thalamic nuclei. Expression of HuD was observed to be coincident with terminal differentiation of all neuronal structures evaluated regardless of the timing of their development, providing correlative evidence for a role in neuronal differentiation or the maintenance of neuronal phenotype. The marked restriction of HuD mRNA expression with maturity suggests that its functional role in adult neurons varies significantly throughout the CNS.
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In the developing mouse and rat cerebral cortex, HuD mRNA is present in proliferating neuronal stem/progenitor cells in the ventricular zone, neuroblasts migrating in the intermediate zones, and terminally differentiating neurons in the cortical plate.
“In the developing mouse and rat cerebral cortex, HuD mRNA is present in proliferating neuronal stem/progenitor cells in the ventricular zone, neuroblasts migrating in the intermediate zones, and terminally differentiating neurons in the cortical plate (Okano and Darnell 1997; Clayton et al. 1998).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Clayton et al. 1998
HuD mRNA levels are detectable by day 10 of mouse and rat embryonic brain development, peak at E16, and slowly decline until birth.
“Multiple laboratories have demonstrated via Northern blots and quantitative RT-PCR that HuD mRNA levels are detectable by day 10 of mouse and rat embryonic brain development, peak at E16, and slowly decline until birth (Clayton et al. 1998; Hambardzumyan et al. 2009; Abdelmohsen et al. 2010).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Clayton et al. 1998
In the adult mouse and rodent nervous system, HuD mRNA expression is mostly restricted to specific neuronal populations, including large pyramidal-like neurons in layer V of the neocortex and cerebellar cortex (Purkinje cells), the four Cornu Amonis (CA1-4) regions of the hippocampus, dorsal root ganglia, motor neurons in the spinal cord, mitral cells in the olfactory bulb, ganglion and internal P
“In the adult mouse and rodent nervous system, expression of HuD mRNA is mostly restricted to specific neuronal populations, including large pyramidal-like neurons in layer V of the neocortex and cerebellar cortex (Purkinje cells), the four Cornu Amonis (CA1-4) regions of the hippocampus, dorsal root ganglia, and motor neurons in the spinal cord, mitral cells in the olfactory bulb, ganglion and int”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Clayton et al. 1998
Cites
Altman, J., & Bayer, S. A. (1990) · Journal of Comparative Neurology · doi:10.1002/cne.903010304 · Cited by 819 · see citing works
A conserved family of elav-like genes in vertebrates.
Good, P. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.10.4557 · Cited by 319 · see citing works
Paraneoplastic Encephalomyelitis Antigens Bind to the AU-rich Elements of mRNA
Liu, J., Dalmau, J., Szabo, A., Rosenfeld, M., Huber, J., & Furneaux, H. (1995) · Neurology · doi:10.1212/wnl.45.3.544 · Cited by 122 · see citing works
Sakai, K., Gofuku, M., Kitagawa, Y., Ogasawara, T., Hirose, G., Yamazaki, M., Koh, C. S., Yanagisawa, N., & Steinman, L. (1994) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1994.1358 · Cited by 85 · see citing works
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Defining a neuron: neuronal ELAV proteins
Pascale, A., Amadio, M., & Quattrone, A. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7017-y · Cited by 158 · see citing works
Neuronal ELAV proteins enhance mRNA stability by a PKCα-dependent pathway
Pascale, A., Amadio, M., Scapagnini, G., Lanni, C., Racchi, M., Provenzani, A., Govoni, S., Alkon, D. L., & Quattrone, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504702102 · Cited by 129 · see citing works
Bronicki, L. M., & Jasmin, B. J. (2013) · RNA · doi:10.1261/rna.039164.113 · Cited by 126 · see citing works
Perfluorododecanoic Acid Exposure Induced Developmental Neurotoxicity in Zebrafish Embryos
Guo, X., Zhang, S., Lu, S., Zheng, B., Xie, P., Chen, J., Li, G., Liu, C., Wu, Q., Cheng, H., et al. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.06.013 · Cited by 58 · see citing works
RNA–Binding Protein HuD as a Versatile Factor in Neuronal and Non–Neuronal Systems
Jung, M., & Lee, E. K. (2021) · Biology · doi:10.3390/biology10050361 · Cited by 39 · see citing works
- Authors
- Clayton, G. H., Perez, G. M., Smith, R. L., & Owens, G. C.
- Affiliations
- University of Colorado Health (US)
- Corresponding author
- Clayton, Gerald H
- Venue
- Developmental Brain Research
- Publisher
- Elsevier BV
- Published
- 1998-08
- Volume
- 109
- Issue
- 2
- Pages
- 271-280
- ISSN
- 0165-3806 (ISSN-L)
- OpenAlex
- W2070542531
- Cited by
- 38
- References
- 4
- Citations by year
- 2024: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 0.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1k08ns001741-01)
- National Institute of Neurological Disorders and Stroke (T32NS007321, NS01741, T32-NS07321-08)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32 HD07408, T32HD007408)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- HUD, ELAVL4, RNA-binding proteins, brain development, neuronal differentiation, mRNA expression, pyramidal neurons, thalamic nuclei, paraneoplastic encephalomyelitis, sensory neuronopathy, postnatal development, rats
- MeSH terms
- Animals, Nerve Tissue Proteins, Nervous System, Ribonucleases, RNA, Messenger, RNA-Binding Proteins, Rats, Sprague-Dawley, In Situ Hybridization, Pyramidal Cells, Gene Expression Regulation, Developmental, Rats, ELAV Proteins
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