Beneficial effects of α-lipoic acid and ascorbic acid on endothelium-dependent, nitric oxide-mediated vasodilation in diabetic patients: relation to parameters of oxidative stress
Abstract
The impairment of nitric oxide (NO)-mediated vasodilation in diabetes has been attributed to increased vascular oxidative stress. Lipoic acid has been shown to have substantial antioxidative properties. The aim of this study was to assess the effect of lipoic acid on NO-mediated vasodilation in diabetic patients in comparison with the well-recognized effect of ascorbic acid. Using venous occlusion plethysmography, we examined the effects of lipoic acid (0.2 mM) and ascorbic acid (1 and 10 mM) on forearm blood flow responses to acetylcholine, sodium nitroprusside and concomitant infusion of the NO-inhibitor, N(G)-monomethyl-L-arginine, in 39 diabetic patients and 11 control subjects. Plasma levels of antioxidants and parameters of lipid peroxidation were measured and correlated to endothelial function tests. Lipoic acid improved NO-mediated vasodilation in diabetic patients, but not in controls. NO-mediated vasodilation was improved by ascorbic acid at 10 mM, but not 1 mM. Improvements of endothelial function by ascorbic acid and lipoic acid were closely related. The beneficial effects of lipoic acid were positively related to plasma levels of malondialdehyde and inversely related to levels of ubiquinol-10. These findings support the concept that oxidative stress contributes to endothelial dysfunction and suggest a therapeutic potential of lipoic acid particularly in patients with imbalance between increased oxidative stress and depleted antioxidant defense.
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Treatment with antioxidants improves endothelial function in certain conditions.
“As the treatment with antioxidants has been shown to improve endothelial function in certain conditions (56, 419, 420, 422, 572), it is possible that the coadministration of BH4 with ascorbic acid or other antioxidants can circumvent these issues.”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [419]
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- Authors
- Heitzer, T.
- Affiliations
- Universität Hamburg (DE)
- Corresponding author
- Heitzer, T
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2001-07-01
- Volume
- 31
- Issue
- 1
- Pages
- 53-61
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2117456991
- Cited by
- 194
- References
- 42
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 3 · 2021: 11 · 2020: 13 · 2019: 8 · 2018: 8 · 2017: 4 · 2016: 4 · 2015: 11 · 2014: 15 · 2013: 14 · 2012: 10
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Biochemical Acid Research Studies
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- alpha-lipoic acid, vitamin C, nitric oxide, vasodilation, endothelial dysfunction, oxidative stress, diabetes mellitus, lipid peroxidation, malondialdehyde, ubiquinol-10, antioxidant defense, acetylcholine, L-NMMA, forearm blood flow
- MeSH terms
- Acetylcholine, Antioxidants, Ascorbic Acid, Blood Flow Velocity, Diabetes Mellitus, Type 2, Endothelium, Vascular, Female, Humans, Thioctic Acid, Male, Middle Aged, Nitric Oxide, Nitroprusside, Plethysmography, Vasodilation, Oxidative Stress
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