Effect of nutrient depletion on β-carotene and glycerol accumulation in two strains of Dunaliella sp.
Abstract
The response of two strains of Dunaliella, a beta-carotene accumulating halotolerant alga, was evaluated under sulphate, nitrate and phosphate limitation. All these factors decreased the growth rate and chlorophyll content but, increased the beta-carotene content of the two isolates of Dunaliella, D1, obtained from GTCC and D2 an indigenous strain isolated from Sambhar salt lake, India. Both the strains exhibited accumulation of beta-carotene and glycerol under the different nutrient limiting conditions. A maximum accumulation of 3.99 pg/cell of beta-carotene was observed under phosphate depletion. However, nutrient depletion did not significantly affect the glycerol accumulation in these cells. D2, the indigenous isolate, was found to be a better accumulator of beta-carotene than D1.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Nutrient deprivation can increase carotenoid production.
“Although studies have shown that nutrient deprivation can increase carotenoid production [66,67], it is also known that the primary carotenoids which are associated with growth are degraded under stress [68,69].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [67]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
ABSORPTION OF LIGHT BY CHLOROPHYLL SOLUTIONS
Mackinney, G. (1941) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51320-x · Cited by 4,018 · see citing works
On the Chemical Composition of Eleven Species of Marine Phytoplankters
PARSONS, T. R., Stephens, K., & Strickland, J. (1961) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f61-063 · Cited by 644 · see citing works
Shifrin, N. S., & Chisholm, S. W. (1981) · Journal of Phycology · doi:10.1111/j.1529-8817.1981.tb00865.x · Cited by 488 · see citing works
RAPID METHOD FOR ESTIMATION OF GLYCEROL IN FERMENTATION SOLUTIONS
Lambert, M. M. T., & Neish, A. C. (1950) · Canadian Journal of Research/Canadian journal of research · doi:10.1139/cjr50b-013 · Cited by 465 · see citing works
Chemical Composition of Acid Fog
Waldman, J. M., Munger, J. W., Jacob, D. J., Flagan, R. C., Morgan, J. J., & Hoffmann, M. R. (1982) · Science · doi:10.1126/science.218.4573.677 · Cited by 379 · see citing works
Ben‐Amotz, A., & Avron, M. (1983) · PLANT PHYSIOLOGY · doi:10.1104/pp.72.3.593 · Cited by 365 · see citing works
Handbook of Phycological Methods. Physiological and Biochemical Methods
Whitton, B. A. (1979) · Phycologia · doi:10.2216/i0031-8884-18-4-439b.1 · Cited by 309 · see citing works
Handbook of phycological methods: Physiological and biochemical methods:
Raven, J. A. (1979) · Phytochemistry · doi:10.1016/0031-9422(79)83048-4 · Cited by 308 · see citing works
Foote, C. S., Denny, R. W., Weaver, L., CHANG, Y., & Peters, J. (1970) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1970.tb39317.x · Cited by 304 · see citing works
Scheer, H. (2013) · Encyclopedia of Biological Chemistry · doi:10.1016/b978-0-12-378630-2.00134-1 · Cited by 220 · see citing works
GLYCEROL AS A PHOTOSYNTHETIC PRODUCT IN DUNALIELLA TERTIOLECTA BUTCHER
Craigie, J. S., & McLachlan, J. (1964) · Canadian Journal of Botany · doi:10.1139/b64-068 · Cited by 101 · see citing works
Ben‐Amotz, A. (1987) · Journal of Plant Physiology · doi:10.1016/s0176-1617(87)80290-0 · Cited by 59 · see citing works
Thomas, W. H., Seibert, D. L. R., Alden, M. J., Neori, A., & Eldridge, P. M. (1984) · Biomass · doi:10.1016/0144-4565(84)90022-2 · Cited by 53 · see citing works
Flagged first, then by citations
1 of the 14 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Markou, G., & Nerantzis, E. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.07.011 · Cited by 815 · see citing works
Aksu, Z., & Eren, A. T. (2005) · Process Biochemistry · doi:10.1016/j.procbio.2005.01.011 · Cited by 271 · see citing works
Microbial consortia: a critical look at microalgae co-cultures for enhanced biomanufacturing
Padmaperuma, G., Kapoore, R. V., Gilmour, D. J., & Vaidyanathan, S. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2017.1390728 · Cited by 186 · see citing works
Stresses as First-Line Tools for Enhancing Lipid and Carotenoid Production in Microalgae
Shi, T.-Q., Wang, L.-R., Zhang, Z.-X., Sun, X.-M., & Huang, H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00610 · Cited by 182 · see citing works
Bhowmick, G. D., Koduru, L., & Sen, R. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.04.131 · Cited by 147 · see citing works
Production of carotenoids by the isolated yeast of Rhodotorula glutinis
Aksu, Z., & Eren, A. T. (2007) · Biochemical Engineering Journal · doi:10.1016/j.bej.2007.01.004 · Cited by 132 · see citing works
Genomic plasticity in prokaryotes: the case of the square haloarchaeon
Cuadros-Orellana, S., Martín-Cuadrado, A.-B., Legault, B. A., D’Auria, G., Zhaxybayeva, O., Papke, R. T., & Rodríguez‐Valera, F. (2007) · The ISME Journal · doi:10.1038/ismej.2007.35 · Cited by 113 · see citing works
Mishra, A., Mandoli, A., & Jha, B. (2008) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0387-9 · Cited by 105 · see citing works
Mojaat, M., Pruvost, J., Foucault, A. P., & Legrand, J. (2007) · Biochemical Engineering Journal · doi:10.1016/j.bej.2007.09.009 · Cited by 98 · see citing works
Srinivasan, R., Mageswari, A., Subramanian, P., Suganthi, C., Chaitanyakumar, A., Aswini, V., & Gothandam, K. M. (2018) · Scientific Reports · doi:10.1038/s41598-018-25417-5 · Cited by 96 · see citing works
Morowvat, M. H., & Ghasemi, Y. (2016) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2016.06.008 · Cited by 95 · see citing works
Wu, Z., Duangmanee, P., Zhao, P., Juntawong, N., & Ma, C. (2016) · Jundishapur Journal of Microbiology · doi:10.5812/jjm.26732 · Cited by 73 · see citing works
Glycerol metabolism in hypersaline environments
Oren, A. (2016) · Environmental Microbiology · doi:10.1111/1462-2920.13493 · Cited by 71 · see citing works
Ruiz‐Domínguez, M. C., Vaquero, I., Obregón, V., Morena, B. D. L., Vı́lchez, C., & Vega, J. M. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0403-6 · Cited by 71 · see citing works
Saejung, C., & Salasook, P. (2018) · Environmental Technology · doi:10.1080/09593330.2018.1491633 · Cited by 67 · see citing works
Saxena, P., & Bassi, A. S. (2012) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.3912 · Cited by 59 · see citing works
Çelekli̇, A., & Dönmez, G. (2005) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-005-9017-0 · Cited by 56 · see citing works
Isolation of Dunaliella spp. from a hypersaline lake and their ability to accumulate glycerol
Kaçka, A., & Dönmez, G. (2008) · Bioresource Technology · doi:10.1016/j.biortech.2008.02.042 · Cited by 52 · see citing works
Ilieva, Y. E., Zaharieva, M. M., Najdenski, H. M., & Kroumov, A. D. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25105548 · Cited by 53 · see citing works
A media design program for lactic acid production coupled with extraction by electrodialysis
Lee, K. (2005) · Bioresource Technology · doi:10.1016/j.biortech.2004.11.010 · Cited by 41 · see citing works
Microalgal pigment induction and transfer in aquaculture
Pérez‐Legaspi, I. A., Valadez-Rocha, V., Ortega‐Clemente, L. A., & Jiménez‐García, M. I. (2019) · Reviews in Aquaculture · doi:10.1111/raq.12384 · Cited by 34 · see citing works
Song, D., Xi, B., & Sun, J. K. (2016) · Journal of Ocean University of China · doi:10.1007/s11802-016-2797-z · Cited by 34 · see citing works
Modeling of lutein production by heterotrophic Chlorella in batch and fed-batch cultures
Wu, Z., Shi, C., & Shi, X. (2007) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-007-9354-2 · Cited by 34 · see citing works
Fazeli, M. R., Tofighi, H., Samadi, N., Jamalifar, H., & Fazeli, A. (2006) · DARU Journal of Pharmaceutical Sciences · Cited by 30 · see citing works
Microalgal swimming signatures and neutral lipids production across growth phases
You, J., Mallery, K., Mashek, D. G., Sanders, M. A., Hong, J., & Hondzo, M. (2020) · Biotechnology and Bioengineering · doi:10.1002/bit.27271 · Cited by 27 · see citing works
Chen, Y., Bi, C., Zhang, J., Hou, H., & Gong, Z. (2020) · South African Journal of Botany · doi:10.1016/j.sajb.2020.07.008 · Cited by 25 · see citing works
Farhat, N., Rabhi, M., Falleh, H., Jouini, J., Abdelly, C., & Smaoui, A. (2011) · Journal of Phycology · doi:10.1111/j.1529-8817.2011.01036.x · Cited by 25 · see citing works
Taxonomy and Phylogeny of the Genus Dunaliella
Gonz√°lez, M., G�mez, P., & Polle, J. W. (2009) · Science Publishers eBooks · doi:10.1201/b10300-3 · Cited by 24 · see citing works
Esther Elizabeth Grace, C., Briget Mary, M., Vaidyanathan, S., & Srisudha, S. (2022) · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2021.120830 · Cited by 21 · see citing works
El-Sheekh, M. M., Khairy, H. M., Gheda, S. F., & El‐Shenody, R. A. (2015) · The Egyptian Journal of Aquatic Research · doi:10.1016/j.ejar.2015.10.001 · Cited by 21 · see citing works
Xu, T., Mitra, R., Tan, D., Li, Z., Zhou, C., Chen, T., Xie, Z., & Han, J. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108302 · Cited by 18 · see citing works
Rukminasari, N. (2013) · International Journal of Marine Science · doi:10.5376/ijms.2013.03.0017 · Cited by 18 · see citing works
Optimization of carotenoids by Rhodotorula glutinis
Chandi, G. K., Singh, S. P., Gill, B. S., Sogi, D. S., & Singh, P. (2010) · Food Science and Biotechnology · doi:10.1007/s10068-010-0125-8 · Cited by 17 · see citing works
Self-citationNew lipid‐producing, cold‐tolerant yellow‐green alga isolated from the rocky mountains of colorado
Nelson, D. R., Mengistu, S., Ranum, P. T., Celio, G. J., Mashek, M. T., Mashek, D. G., & Lefebvre, P. A. (2013) · Biotechnology Progress · doi:10.1002/btpr.1755 · Cited by 15 · see citing works
Carotenoid Biosynthesis in Dunaliella (Chlorophyta)
Jin, E., & Polle, J. W. (2009) · Science Publishers eBooks · doi:10.1201/b10300-7 · Cited by 15 · see citing works
Carotene and Antioxidant Capacity of Dunaliella Salina Strains
Võ, T., & Tran, D. (2014) · World Journal of Nutrition and Health · doi:10.12691/jnh-2-2-2 · Cited by 13 · see citing works
Chantzistrountsiou, X., Ntzouvaras, A., Papadaki, S., Tsirigoti, A., Tzovenis, I. P., & Economou‐Amilli, A. (2023) · Water · doi:10.3390/w15020241 · Cited by 12 · see citing works
Stress responses of the green microalga, Dunaliella salina to PEG-induced drought
Tafvizi, F., Tafreshi, S. A. H., Toluei, Z., & Toghyani, M. A. (2020) · Journal of the Marine Biological Association of the United Kingdom · doi:10.1017/s0025315420000971 · Cited by 12 · see citing works
Wu, Z., Akter, R., Arirob, W., Juntawong, N., Ma, C., & Duangmanee, P. (2015) · Microbiology Research · doi:10.4081/mr.2015.6233 · Cited by 12 · see citing works
Carotenoid Biosynthesis in Dunaliella (Chlorophyta)
Jin, E., & Polle, J. E. W. (2019) · CRC Press eBooks · doi:10.1201/9780429061639-6 · Cited by 12 · see citing works
Tafreshi, S. A. H., Aghaie, P., Toghyani, M. A., & Ramazani-Moghaddam-Arani, A. (2020) · International Journal of Radiation Biology · doi:10.1080/09553002.2020.1741717 · Cited by 11 · see citing works
AbuSara, N. F., Emeish, S., & Sallal, A. J. (2011) · Jordan Journal of Biological Sciences · Cited by 9 · see citing works
Ghosh, A., Khanra, S., Haldar, G., Bhowmick, T. K., & Gayen, K. (2018) · Current Biochemical Engineering · doi:10.2174/2212711905666180817105828 · Cited by 9 · see citing works
Brennan, L., Mostaert, A. S., Murphy, C. D., & Owende, P. M. O. (2012) · Wiley eBooks · doi:10.1002/9780470976692.ch10 · Cited by 9 · see citing works
Xi, Y., Xue, S., Cao, X., Chi, Z., & Wang, J. (2021) · Bioresources and Bioprocessing · doi:10.1186/s40643-021-00457-4 · Cited by 6 · see citing works
Evaluation of Chlorella vulgaris grown in sugar industry wastewater for use as aquaculture feed
Saejung, C., & Ektasaeng, T. (2022) · International Journal of Environmental Science and Technology · doi:10.1007/s13762-022-04388-1 · Cited by 7 · see citing works
Cultivation, Growth Media, Division Rates and Applications of Dunaliella Species
Rao, D. S. (2009) · Science Publishers eBooks · doi:10.1201/b10300-4 · Cited by 7 · see citing works
Võ, T., Mai, T., Vu, H., Van, D., Dao, H. T. T., Tran, P., Nguyen, N., Nguyen, P., & Nguyen, N. C. (2017) · Research in Plant Sciences · doi:10.12691/plant-5-1-1 · Cited by 4 · see citing works
Keil, L., Qoura, F., Breitsameter, J. M., Rieger, B., Garbe, D., & Brück, T. (2024) · Microorganisms · doi:10.3390/microorganisms12071318 · Cited by 3 · see citing works
Riouchi, O., Kholssi, R., Skalli, A., Akodad, M., Moumen, A., & Baghour, M. (2024) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2024.103088 · Cited by 3 · see citing works
Bühler, R. M. M. (2008) · Americanae (AECID Library) · Cited by 3 · see citing works
Optimization of the glycerol production from Dunaliella tertiolecta and Dunaliella isolatesPreprint
Keil, L., Breitsameter, J. M., Rieger, B., Garbe, D., & Brück, T. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.09.18.558238 · Cited by 2 · see citing works
Identification of Dunaliella Viridis Using its Markers
Tran, D., Louime, C. J., Võ, T., Giordano, M., Portilla, S. E., Doan, N. T., Tran, D. Q., Mai, T., & Le, B. V. (2013) · Università Politecnica delle Marche (Università Politecnica delle Marche) · Cited by 1 · see citing works
Nelson, D. R. (2012) · University of Minnesota Digital Conservancy (University of Minnesota)
Riyahi, J., Haouazine, Y., Akallal, R., Mouradi-Givernaud, A., Créach, A., Mouradi-Givernaud, A., & Méthodes, M. E. (2008)
Jeyanthi, S., & Santhanam, P. (2018) · Springer eBooks · doi:10.1007/978-981-10-7938-2_7
Microalgal swimming signatures as indicators of neutral lipids production across growth phasesPreprint
You, J., Mallery, K., Mashek, D. G., Sanders, M. A., Hong, J., & Hondzo, M. (2019) · arXiv (Cornell University) · doi:10.48550/arxiv.1911.01853
Nikookar, K., Moghaddam, A. G., Niazi, A., Kholdebarin, B., & Mohsenzadeh, S. (2017) · Iranian Journal of Science and Technology Transactions A Science · doi:10.1007/s40995-017-0290-1
Hazeem, L. J. (2014) · Arab Gulf Journal of Scientific Research · doi:10.51758/agjsr-01-2014-0006
Klin, M., Pniewski, F., & Latała, A. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7610483
- Authors
- Phadwal, K., & Singh, P. K.
- Affiliations
- Indian Agricultural Research Institute (IN)
- Corresponding author
- Phadwal, Kanchan
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 90
- Issue
- 1
- Pages
- 55-58
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2017148783
- Cited by
- 62
- References
- 14
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 3
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Indian Agricultural Research Institute
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Keywords
- Dunaliella, β-carotene accumulation, glycerol accumulation, nutrient limitation, sulfate limitation, nitrate limitation, phosphate limitation, halotolerant algae, chlorophyll content, algal growth rate, Sambhar Salt Lake, carotenoid biosynthesis
- MeSH terms
- Chlorophyta, Cells, Cultured, Chlorophyll, Culture Media, Glycerol, Nitrates, Phosphates, Species Specificity, Sulfates, beta Carotene
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
