Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga

Lamers, Packo P.; Janssen, Marcel; De Vos, Ric C.H.; Bino, Raoul J.; Wijffels, René H.

doi:10.1016/j.jbiotec.2012.04.018

Cited by 216Algal biology and biofuel productionPlant biochemistry and biosynthesisLipid metabolism and biosynthesis

Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga

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Lamers, Packo P., Janssen, Marcel, De Vos, Ric C.H., Bino, Raoul J., Wijffels, René H.·2012·Journal of Biotechnology

DOI 10.1016/j.jbiotec.2012.04.018

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Abstract

Nitrogen availability and light intensity affect β-carotene overproduction in the green alga Dunaliella salina. Following a previous study on high-light stress, we here report on the effect of nitrogen depletion on the growth characteristics and β-carotene as well as fatty acid metabolism of D. salina under a constant light regime in a turbidostat. Upon nitrogen depletion, the biomass yield on absorbed light approximately doubled, due to a transient increase in cell division rate, swelling of the cells and a linear increase of the density of the cells. Simultaneously, β-carotene started to accumulate up to a final intracellular concentration of 14 mg LCV⁻¹ (i.e. 2.7% of AFDW). This β-carotene production accounted for 6% of the increased density of the cells, indicating that other biochemical constituents accumulated as well. Since D. salina accumulates β-carotene in lipid globules, we also determined the fatty acid content and composition of D. salina. The intracellular concentration of the total fatty acid pool did not change significantly during nitrogen starvation, indicating that β-carotene and total fatty acid accumulation were unrelated, similar to what was found previously for high-light treated cells. However, for both high-light and nitrogen stress, β-carotene accumulation negatively correlated with the degree of unsaturation of the total fatty acid pool and, within the individual fatty acids, correlated positively with oleic acid biosynthesis, suggesting that oleic acid may be a key component of the lipid-globule-localized triacylglycerols and thereby in β-carotene accumulation.

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Oxidative damage caused by nitrogen deficiency, to which PUFA is particularly sensitive, may be related to the changes in fatty acid composition.
“This may be related to oxidative damage caused by nitrogen deficiency, to which PUFA is particularly sensitive [117].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [117]
Dunaliella salina can produce carotenoids at approximately 10% of its total dry weight under stress conditions such as high salinity, high light intensity, nutrient depletion, or extreme temperatures.
“This halotolerant microalga can produce high contents of carotenoids, approximately 10% of its total dry weight, under stress conditions such as high salinity, high light intensity, nutrient depletion, or extreme temperatures (Fig. 3) (Ben‐Amotz, 1983; Borowitzka et al., 1990; Lamers et al., 2012).”
From Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry (2022) · cites this paper as Lamers et al., 2012

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Authors: Lamers, P. P., Janssen, M., De Vos, R. C. H., Bino, R. J., & Wijffels, R. H.
Affiliations: Wageningen University & Research (NL)
Centre for BioSystems Genomics (NL)
Corresponding author: Lamers, Packo P.
ORCID: Lamers, Packo P.
Janssen, Marcel
Wijffels, René H.
Venue: Journal of Biotechnology
Publisher: Elsevier BV
Published: 2012-11
Volume: 162
Issue: 1
Pages: 21-27
ISSN: 0168-1656 (ISSN-L)
DOI: 10.1016/j.jbiotec.2012.04.018
OpenAlex: W2082831560
Cited by: 216
References: 33
Citations by year: 2026: 10 · 2025: 8 · 2024: 12 · 2023: 11 · 2022: 23 · 2021: 21 · 2020: 19 · 2019: 19 · 2018: 16 · 2017: 17 · 2016: 18 · 2015: 17 · 2014: 12 · 2013: 11 · 2012: 1
FWCI: 5.03
Type: journal article
Language: en
Open access: green
research.wur.nl
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Journal of Biotechnology (publishedVersion)
PubMed (publishedVersion)
Socio-Environmental Systems Modeling (acceptedVersion)
Data Archiving and Networked Services (DANS) (submittedVersion)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: Dunaliella salina, nitrogen starvation, β-carotene accumulation, fatty acid metabolism, oleic acid biosynthesis, lipid globules, triglycerides, fatty acid unsaturation, microalgae growth, cell division rate, high light stress
MeSH terms: Fatty Acids, Nitrogen, Stress, Physiological, Volvocida, Biomass, beta Carotene, Intracellular Space, Cell Growth Processes, Microalgae

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Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga

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