The DOI matches its Crossref record; 45 of the 47 references it cites were found in a registry or an index.
Technological advances in high-throughput screening
Abstract
A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactions. Modifications have been developed for cell-free, homogeneous assay systems, such as time-resolved fluorescence, fluorescence polarization and the scintillation proximity assay. Innovations in engineering and chemistry have led to delivery systems for nanoliter volumes and sensitive biosensors for ultra-high-throughout screening conducted in nanoliter and picoliter volumes. Spectroscopic methods have been extended to read single molecule fluorescence. Technologies are being developed to identify new targets from genomic information in order to design the next generation of screens.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Citations
Cites
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Miyawaki, A., Llopis, J. L., Heim, R., McCaffery, J. M., Adams, J. A., Ikura, M., & Tsien, R. Y. (1997) · Nature · doi:10.1038/42264 · Cited by 3,250
On/off blinking and switching behaviour of single molecules of green fluorescent protein
Dickson, R. M., Cubitt, A. B., Tsien, R. Y., & Moerner, W. E. (1997) · Nature · doi:10.1038/41048 · Cited by 1,366
Fei, H., Okano, H. J., Li, C., Lee, G.-H., Zhao, C., Darnell, R., & Friedman, J. M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.7001 · Cited by 766
Zlokarnik, G., Negulescu, P. A., Knapp, T. E., Mere, L., Burres, N. S., Feng, L., Whitney, M. A., Roemer, K., & Tsien, R. Y. (1998) · Science · doi:10.1126/science.279.5347.84 · Cited by 691
Optical Amplification of Ligand-Receptor Binding Using Liquid Crystals
Gupta, V. K., Skaife, J. J., Dubrovsky, T. B., & Abbott, N. L. (1998) · Science · doi:10.1126/science.279.5359.2077 · Cited by 476
Romoser, V. A., Hinkle, P. M., & Persechini, A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.20.13270 · Cited by 398
Applications of the green fluorescent protein in cell biology and biotechnology
Misteli, T., & Spector, D. L. (1997) · Nature Biotechnology · doi:10.1038/nbt1097-961 · Cited by 357
Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy
Eggeling, C., Fries, J. R., Brand, L., Guenther, R., & Seidel, C. A. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.4.1556 · Cited by 341
Molecular Photoionic AND Logic Gates with Bright Fluorescence and “Off−On” Digital Action
de Silva, A. P., Gunaratne, H. Q. N., & McCoy, C. P. (1997) · Journal of the American Chemical Society · doi:10.1021/ja9712229 · Cited by 328
Fluorescence correlation spectroscopy: Diagnostics for sparse molecules
Maiti, S., Haupts, U. M., & Webb, W. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.22.11753 · Cited by 321
Research works printed but not linked: 11
- Razvi, 1998, The current status of high throughput screening and outlook for the future, part 1 (of outstanding interest), Drug Market Dev, 9, 89
- Sannes, 1997, Advances in high throughput screening (of outstanding interest), Spectrum: Drug Discovery and Design. Decision Resources, 8, 1
- Alderan, 1998, A novel approach to ultra-high-throughput screening, Gen Eng News, 18, 14
- Haberman, 1997, Genomics-based drug discovery: from gene sequencing to biology, Decision Resources, 7, 1
- Ashton, 1998, Proteomics — the new watchword for biotech (of outstanding interest), Scrip, 65, 32
- Kajkowski, 1997, Investigation of growth hormone releasing hormone releasing hormone receptor structure and activity using yeast expression technologies, J Recept Signal Transduct Res, 17, 293, 10.3109/10799899709036610 · DOI
- Fox, 1996, Packard applies HTRF tool to high-throughput screens, Gen Eng News, 16, 1
- Brandt, 1998, Microfluidics & high density microplates, Gen Eng News, 18, 30
- Krul, 1997, The commercialization of high-throughput screening technology (of outstanding interest), Spectrum, 143/1
- Zipkin, 1998, Orchid: microchemical processors, BioCentury, 6, 9
- Felder, 1997, High throughput screening and other applications of laboratory automation from LabAutomation ′97, Am Lab, 29, 26
Cited by
Sorted by citations
Items 101–172 of 172
A high-throughput method for fitting dose–response curves using Microsoft Excel
Parsons, J. D. (2006) · Analytical Biochemistry · doi:10.1016/j.ab.2006.09.033 · Cited by 11
A nonradioactive DNA methyltransferase assay adaptable to high-throughput screening
Woo, Y., Rajagopalan, P. T. R., & Benkovic, S. J. (2005) · Analytical Biochemistry · doi:10.1016/j.ab.2005.01.059 · Cited by 11
Schwartz-Mittelmann, A., Neufeld, T., Biran, D., & Rishpon, J. (2003) · Analytical Biochemistry · doi:10.1016/s0003-2697(03)00110-6 · Cited by 10
High‐Throughput Screening for Lead Discovery
Houston, J. G., & Banks, M. N. (2003) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc020 · Cited by 10
Microbial genomics – new targets, new drugs
Schmid, M. B. (2001) · Expert Opinion on Therapeutic Targets · doi:10.1517/14728222.5.4.465 · Cited by 9
Bioactive Compounds from the Genus Broussonetia
Lee, D., & Kinghorn, A. D. (2003) · Studies in natural products chemistry · doi:10.1016/s1572-5995(03)80137-0 · Cited by 8
APPLICATIONS OF NUCLEAR MAGNETIC RESONANCE AND MASS SPECTROMETRY TO ANTICANCER DRUG DISCOVERY
Powers, R., & Siegel, M. M. (2006) · Elsevier eBooks · doi:10.1016/b978-012088561-9/50006-5 · Cited by 8
NMR study of fumarprotocetraric acid, a complex lichen depsidone derivative from Cladonia furcata
Su, B., Cuendet, M., Nikolic, D. S., Kristinsson, H. G., Ingólfsdóttir, K., van Breemen, R. B., Fong, H. H. S., Pezzuto, J. M., & Kinghorn, A. D. (2003) · Magnetic Resonance in Chemistry · doi:10.1002/mrc.1181 · Cited by 8
Image-Based Screening of Signal Transduction Assays
Ramm, P., & Thomas, N. (2003) · Science s STKE · doi:10.1126/stke.2003.177.pe14 · Cited by 8
Han, S., Joo, Y., & Han, K.-H. (2012) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2012.05.042 · Cited by 7
Molecular recognition: Identifying compounds and their targets
FERNANDES, P. B. (2001) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10060 · Cited by 7
Self-citationApplication of the yeast two-hybrid system in molecular gerontology
Knudsen, C. R., Jadidi, M., Friis, I., & Mansilla, F. (2002) · Biogerontology · doi:10.1023/a:1016219121897 · Cited by 7
Jayaraman, A., Yarmush, M. L., & Roth, C. M. (2001) · Industrial & Engineering Chemistry Research · doi:10.1021/ie0102549 · Cited by 7
Larocque, G. R., Mailly, D., Yue, T. ‐., Anand, M., Peng, C. H., Kazancı, C., Etterson, M. A., Goethals, P. L. M., Jørgensen, S. E., Schramski, J. R., et al. (2011) · Ecological Modelling · doi:10.1016/j.ecolmodel.2010.12.017 · Cited by 6
Rossant, C. J., Matthews, C., Neal, F., Colley, C. S., Gardener, M. J., & Vaughan, T. J. (2014) · SLAS DISCOVERY · doi:10.1177/1087057114557464 · Cited by 6
Vogel, H. G. (2007) · Springer eBooks · doi:10.1007/978-3-540-70995-4_12 · Cited by 6
Kosmeder, J. W., & Pezzuto, J. M. (2001) · Cancer treatment and research · doi:10.1007/978-1-4615-1657-6_2 · Cited by 6
Automated and intelligent chemical synthesis
Yu, L., Lin, C., Zhou, D., Cao, L., & Wang, C. (2022) · Scientia Sinica Chimica · doi:10.1360/ssc-2022-0192 · Cited by 5
A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals
Rivera, B. N., Wilson, L. B., Kim, D. N., Pande, P., Anderson, K. A., Tilton, S. C., & Tanguay, R. L. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph19073829 · Cited by 5
Deosarkar, S. P., Bhatt, P. M., Gillespie, J. L., Goetz, D. J., & McCall, K. D. (2014) · Drug Development Research · doi:10.1002/ddr.21231 · Cited by 5
Wu, S., Yao, C., Hsieh, C., Liu, Y.-W., Chao, Y., Song, J., & Lee, J. (2015) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2015.03.011 · Cited by 5
Nanodevices for Single Molecule Studies
Craighead, H. G., Stavis, S. M., & Samiee, K. T. (2007) · Springer eBooks · doi:10.1007/3-540-49522-3_13 · Cited by 5
Current insights into computer-aided immunotherapeutic design strategies
Cai, Z. D., Zhang, G., Zhang, X., Liu, Y., & Fu, X. (2015) · International Journal of Immunopathology and Pharmacology · doi:10.1177/0394632015588765 · Cited by 5
Analyses of RNA–Ligand Interactions by Fluorescence Anisotropy
Kishor, A., Brewer, G., & Wilson, G. M. (2012) · Springer eBooks · doi:10.1007/978-1-4614-3991-2_9 · Cited by 4
Bioactive Constituents of the Genus Hernandia
(2005) · Studies in natural products chemistry · doi:10.1016/s1572-5995(05)80042-0 · Cited by 4
Shan, J., & Zheng, J. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-008-3_2 · Cited by 4
Polymer-Supported Reagents and Scavengers in Synthesis
Ley, S. V., Baxendale, I. R., & Myers, R. M. (2007) · Elsevier eBooks · doi:10.1016/b0-08-045044-x/00108-5 · Cited by 4
Fluorescence Applications in Targeted Drug Delivery
Bryl, K., & Langner, M. (2005) · Springer eBooks · doi:10.1007/3-540-27004-3_13 · Cited by 4
Li, S., Li, W., Xiao, H., Chen, P., & Yang, G. (2006) · Journal of Mathematical Chemistry · doi:10.1007/s10910-006-9073-1 · Cited by 4
Shin, Y. G., Udeani, G., Kosmeder, J. W., Zhao, G., Dagar, S., Önyüksel, H., Moriarty, R. M., Cordell, G. A., Moon, R. C., Kinghorn, A. D., et al. (2004) · Journal of Liquid Chromatography & Related Technologies · doi:10.1081/jlc-120030178 · Cited by 3
Phytochemicals as Potential Cancer Chemopreventive Agents
Mehta, R. G., & Pezzuto, J. M. (2004) · CRC series in modern nutrition science · doi:10.1201/9780203506707.ch19 · Cited by 3
Hidalgo, I. J., & Li, J. (2006) · Informa Healthcare eBooks · doi:10.1201/9781420004236.ch9 · Cited by 3
NMR ‐Based Screening and Drug Discovery
Craik, D. J., Smith, P. A., & Clark, R. J. (2010) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc011.pub2 · Cited by 3
Drug discovery and the intracellular receptor family
Miner, J. N., & Tyree, C. M. (2001) · Vitamins and hormones · doi:10.1016/s0083-6729(01)62007-7 · Cited by 3
Farahani, N. I., Lin, L. D., Nazir, S., Naderi, A., Rokos, L., McIntosh, A. R., & Julian, L. M. (2025) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2024.1478572 · Cited by 4
New Advances in Cell Adhesion Technology
Carnazza, S. (2008) · Springer eBooks · doi:10.1007/978-3-540-70946-6_3 · Cited by 2
Han, S., Joo, Y.-D., & Han, K.-H. (2012) · Transactions of the Korean Society of Mechanical Engineers B · doi:10.3795/ksme-b.2012.36.3.241 · Cited by 2
NMR Screening Methods for Drug Discovery
Shortridge, M. D., & Powers, R. (2011) · IOS Press eBooks · doi:10.3233/978-1-60750-695-9-381 · Cited by 2
Gene Promoters and Transcription Control Regions as Therapeutic Targets
Antonio Zorzano, A., Sebastián, D., Sánchez, J., Miret‐Casals, L., & Alberício, F. (2012) · Wiley eBooks · doi:10.1002/9781118185537.ch9 · Cited by 2
Künzler, T. P. (2007) · Repository for Publications and Research Data (ETH Zurich) · doi:10.3929/ethz-a-005420464 · Cited by 2
Image-Based Screening of Signal Transduction Assays
Ramm, P., & Thomas, N. (2003) · Science Signaling · doi:10.1126/scisignal.1772003pe14 · Cited by 2
The miniaturised biological assay: constraints and limitations
Knibiehler, M. (2011) · Springer eBooks · doi:10.1007/978-3-642-19615-7_3 · Cited by 1
Cell-Based Screening to Identify Cytoprotective Compounds
Gerő, D. (2018) · InTech eBooks · doi:10.5772/intechopen.73172 · Cited by 1
Cancer Drug Development in the Modern Era
Sellers, W. R., & Matter, A. (2004) · doi:10.1002/0471675067.mmc025 · Cited by 1
Lawrence Berkeley National Lab. (LBNL), B., Laurence, T. A., & Basic Energy Sciences (BES) (SC-22) USDOE Office of Science (SC) (2002) · doi:10.2172/813378 · Cited by 1
Kusch-Poddar, M. (2006) · edoc (University of Basel) · doi:10.5451/unibas-003956786 · Cited by 1
Innovations scientifiques et technologiques dans la recherche de nouveaux médicaments
Hélène, C. (1999) · Comptes Rendus de l Académie des Sciences - Series III - Sciences de la Vie · doi:10.1016/s0764-4469(00)88521-2 · Cited by 1
Microarray and Fluidic Chip for Extracellular Sensing
Ozkan, M., Ozkan, C. S., Prasad, S., Yang, M., & Zhang, X. (2007) · Springer eBooks · doi:10.1007/978-0-387-25843-0_3 · Cited by 1
Moving Into the Third Millennium After a Century of Screening
FERNANDES, P. B. (2001) · Drugs and the pharmaceutical sciences · doi:10.1201/9780203908570.ch1 · Cited by 1
Self-citationThe role of organic synthesis in the generation of molecular diversity
Wright, D. L., Orugunty, R. S., & Robotham, C. V. (2001) · Organic synthesis · doi:10.1016/s1047-773x(01)80005-6 · Cited by 1
Seethala, R. (2001) · CRC Press eBooks · doi:10.1201/9780203908570-5 · Cited by 1
Gene expression-based screening of inhibitors of signal transduction
Antipova, A. A. (2005) · DSpace@MIT (Massachusetts Institute of Technology)
Drug Discovery by Aptamers in Protozoan Infectious Diseases
Wrenger, C., & Ulrich, H. (2011) · InTech eBooks · doi:10.5772/28913
The Role of Organic Synthesis in the Generation of Molecular Diversity
Wright, D. L., Orugunty, R. S., & Robotham, C. V. (2003) · ChemInform · doi:10.1002/chin.200321260
Introduction Strategies in Drug Discovery and Evaluation
Vogel, H. G. (2007) · Springer eBooks · doi:10.1007/978-3-540-70995-4_1
THE IN VITRO METABOLISM OF THREE ANTICANCER DRUGS
Fan, Y. (2007) · D-Scholarship@Pitt (University of Pittsburgh)
In Silico-Guided Design of Novel-Scaffold Therapeutics Targeting the Dopamine D3 Receptor
Hailemicael, D. G. (2014)
Ward, W. H. J. (1999) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(99)00002-2
NMR‐Based Screening and Drug Discovery
Craik, D. J., Smith, P. A., & Clark, R. J. (2021) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc011.pub3
Introduction Strategies in drug discovery and evaluation1
Vogel, H. G., Vogel, W. H., Schölkens, B. A., Sandow, J., Müller, G. A., & Vogel, W. F. (2002) · Springer eBooks · doi:10.1007/3-540-29837-1_1
Seethala, R. (2001) · Drugs and the pharmaceutical sciences · doi:10.1201/9780203908570.ch3
Assays for Insulin and Insulin-Like Activity Based on Adipocytes
Müller, G. A. (2015) · Springer eBooks · doi:10.1007/978-3-319-05392-9_154
Assays for Insulin and Insulin-Like Activity Based on Adipocytes
Müller, G. A. (2015) · Springer eBooks · doi:10.1007/978-3-642-27728-3_154-1
A novel USD-modelling tool for chromatography design : specification of resin properties
Tang, A. (2012) · UCL Discovery (University College London)
Rehman, M. U., Rahman, A. U., Irfan, M., & Naureen, F. (2026) · Springer eBooks · doi:10.1007/978-3-642-36202-6_251-1
BindingDB (2009) · UC San Diego · doi:10.7270/q289154f
BindingDB (2009) · UC San Diego · doi:10.7270/q22z14rs
Protein–DNA recognition : in vitro evolution and characterization of DNA-binding proteins.
Nilsson, M. (2004) · KTH Publication Database DiVA (KTH Royal Institute of Technology)
Wess (2001) · doi:10.1002/1521-3773(20010917)40:18
Metadata
- Authors
- Fernandes, P. B.
- Corresponding author
- Fernandes, Prabhavathi B
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 1998-01
- Volume
- 2
- Issue
- 5
- Pages
- 597-603
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W1975477535
- Cited by
- 170
- References
- 47
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 6 · 2012: 6
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Computational Drug Discovery Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- high-throughput screening, cell-based assays, yeast two-hybrid, protein-nucleic acid interactions, ligand-receptor interactions, time-resolved fluorescence, fluorescence polarization, scintillation proximity assay, biosensors, picoliter volumes, single-molecule fluorescence
- MeSH terms
- Calmodulin, DNA, Drug Evaluation, Preclinical, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Fluoresceins, Fluorescence Polarization, Fluorescent Dyes, Indicators and Reagents, Luminescent Proteins, Miniaturization, RNA, Robotics, Saccharomyces cerevisiae, Sensitivity and Specificity, Spectrometry, Fluorescence, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
