The DOI matches its Crossref record; 45 of the 47 references it cites were found in a registry or an index.
Technological advances in high-throughput screening
Abstract
A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactions. Modifications have been developed for cell-free, homogeneous assay systems, such as time-resolved fluorescence, fluorescence polarization and the scintillation proximity assay. Innovations in engineering and chemistry have led to delivery systems for nanoliter volumes and sensitive biosensors for ultra-high-throughout screening conducted in nanoliter and picoliter volumes. Spectroscopic methods have been extended to read single molecule fluorescence. Technologies are being developed to identify new targets from genomic information in order to design the next generation of screens.
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Metadata
- Authors
- Fernandes, P. B.
- Corresponding author
- Fernandes, Prabhavathi B
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 1998-01
- Volume
- 2
- Issue
- 5
- Pages
- 597-603
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W1975477535
- Cited by
- 170
- References
- 47
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 6 · 2012: 6
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Computational Drug Discovery Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- high-throughput screening, cell-based assays, yeast two-hybrid, protein-nucleic acid interactions, ligand-receptor interactions, time-resolved fluorescence, fluorescence polarization, scintillation proximity assay, biosensors, picoliter volumes, single-molecule fluorescence
- MeSH terms
- Calmodulin, DNA, Drug Evaluation, Preclinical, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Fluoresceins, Fluorescence Polarization, Fluorescent Dyes, Indicators and Reagents, Luminescent Proteins, Miniaturization, RNA, Robotics, Saccharomyces cerevisiae, Sensitivity and Specificity, Spectrometry, Fluorescence, Green Fluorescent Proteins
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