Cited by 172Advanced Biosensing Techniques and ApplicationsComputational Drug Discovery MethodsCell Image Analysis Techniques

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Technological advances in high-throughput screening

Fernandes, Prabhavathi B·1998·Current Opinion in Chemical Biology

DOI 10.1016/s1367-5931(98)80089-6

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Abstract

A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactions. Modifications have been developed for cell-free, homogeneous assay systems, such as time-resolved fluorescence, fluorescence polarization and the scintillation proximity assay. Innovations in engineering and chemistry have led to delivery systems for nanoliter volumes and sensitive biosensors for ultra-high-throughout screening conducted in nanoliter and picoliter volumes. Spectroscopic methods have been extended to read single molecule fluorescence. Technologies are being developed to identify new targets from genomic information in order to design the next generation of screens.

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Authors: Fernandes, P. B.
Corresponding author: Fernandes, Prabhavathi B
ORCID: Fernandes, Prabhavathi B
Venue: Current Opinion in Chemical Biology
Publisher: Elsevier BV
Published: 1998-01
Volume: 2
Issue: 5
Pages: 597-603
ISSN: 1367-5931 (ISSN-L)
DOI: 10.1016/s1367-5931(98)80089-6
OpenAlex: W1975477535
Cited by: 170
References: 47
Citations by year: 2026: 1 · 2025: 1 · 2024: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 6 · 2012: 6
FWCI: 1.05
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Current Opinion in Chemical Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Computational Drug Discovery Methods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
Keywords: high-throughput screening, cell-based assays, yeast two-hybrid, protein-nucleic acid interactions, ligand-receptor interactions, time-resolved fluorescence, fluorescence polarization, scintillation proximity assay, biosensors, picoliter volumes, single-molecule fluorescence
MeSH terms: Calmodulin, DNA, Drug Evaluation, Preclinical, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Fluoresceins, Fluorescence Polarization, Fluorescent Dyes, Indicators and Reagents, Luminescent Proteins, Miniaturization, RNA, Robotics, Saccharomyces cerevisiae, Sensitivity and Specificity, Spectrometry, Fluorescence, Green Fluorescent Proteins

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