Microalgae mediated photoproduction of β-carotene in aqueous–organic two phase systems
Abstract
Improving productivity is a usual requirement for most biotechnological processes, and the utilisation of two-phase aqueous organic systems has proved to be an effective way to improve the productivity of poorly water-soluble or toxic compounds. The high hydrophobicity of beta-carotene, which is highly demanded by the pharma and agrofood industry, makes it a good candidate for aqueous/organic biphasic photoproduction. In the present work we have investigated the viability of a two-phase system for the production of beta-carotene by the marine microalgae Dunaliella salina using decane as organic phase. Decane, with a logP(octanol) value of 5.6, showed no toxicity to Dunaliella cells for more than 72 h, and its ability for beta-carotene extraction is acceptable. Transferring Dunaliella cells from standard to carotenogenic conditions caused inhibition of chlorophyll production and induced a strong synthesis of beta-carotene. The two-phase aqueous/decane system was stable and beta-carotene content of the cells was increasing during 4-days. About 8% of the total carotenoids produced were excreted and extracted into the decane phase.
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A diet rich in β-carotene maintains average serum levels and lowers the incidence of several types of cancer and degenerative diseases.
“However, many epidemiological studies suggest that humans fed a diet rich in β-carotene maintained their average serum levels and lowered their incidence of several types of cancer and degenerative diseases (Leon et al., 2003).”
From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Leon et al., 2003
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- Authors
- León, R.
- Affiliations
- Universidad de Huelva (ES)
- Corresponding author
- León, R
- ORCID
- León, R
- Venue
- Biomolecular Engineering
- Publisher
- Elsevier BV
- Published
- 2003-07
- Volume
- 20
- Issue
- 4-6
- Pages
- 177-182
- ISSN
- 1389-0344 (ISSN-L)
- OpenAlex
- W2066504928
- Cited by
- 80
- References
- 15
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 8 · 2012: 5
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomolecular Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Chemical Engineering › Filtration and Separation › Chemical and Physical Properties in Aqueous Solutions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- β-carotene production, Dunaliella salina, microalgae, aqueous-organic two-phase systems, n-decane, carotenoid extraction, photoproduction, hydrophobic compounds, bioprocess productivity
- MeSH terms
- Adaptation, Physiological, Chlorophyta, Alkanes, Cell Division, Cell Survival, Chlorophyll, Dose-Response Relationship, Radiation, Light, Radiation Dosage, Solvents, Water, Cell Culture Techniques, Bioreactors, beta Carotene, Phase Transition
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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