Electrical Impedance Determination of Cancer Cell Viability in a 3-Dimensional Cell Culture Microfluidic Chip
Open accessAbstract
A technique of quantitative determination of cell viability in three-dimensional (3D) cell culture construct is proposed based on on-site electrical impedance measurement in a microfluidic chip. In vitro 3D cell culture has been interpreted for faithfully representation of the in vivo cellular responses in living tissues. However, monitoring of the cellular responses in 3D cell culture construct is normally time-consuming and labor-intensive. In this work, a microfluidic chip was developed and is capable of on-site determining cell viability in 3D construct without affecting the cellular behaviors. The chip mainly consisted of a culture chamber, in which a pair of vertical electrodes at its opposite sidewalls was embedded for measurement, and a fluidic channel for perfusion purpose. Cancer cells/agarose suspension was loaded into the culture chamber for 3D cell culture with perfusing anti-cancer drug in different concentrations (6, 12, 18, and 24 μg ml-1) for 2 days. Since higher drug concentration led to more cell damage or death, the total impedance magnitude of the culture construct was shown to be reasonably proportional to the cell viability. The proposed technique has a high potential to develop a fast and easy measurement compared with the conventional cellular analysis techniques.
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Other researchers have used 3D cell-based electrochemical biosensors to quantify cancer cell resistance to drug compounds and to study cancer cell proliferation in the presence of anticancer drugs.
“Other researchers have used 3D cell-based electrochemical biosensors to quantify cancer cell resistance to drug compounds 78,95 and to study cancer cell proliferation in the presence of anticancer drugs. 94”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [95]
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- Authors
- Lei, K. F., Wu, M.-H., Hsu, C.-W., & Chen, Y.-D.
- Affiliations
- Chang Gung University (TW)
- Corresponding author
- Lei, Kin Fong
- Venue
- International Journal of Electrochemical Science
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 7
- Issue
- 12
- Pages
- 12817-12828
- ISSN
- 1452-3981 (ISSN-L)
- OpenAlex
- W4382394022
- Cited by
- 12
- References
- 31
- Citations by year
- 2026: 1 · 2025: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2017: 2 · 2014: 1 · 2013: 3
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Electrochemical Science (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Ministry of Science and Technology, Taiwan (NSC101-2221-E-182-003-MY3)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- electrical impedance, cell viability, 3D cell culture, microfluidics, cancer cells, agarose, anticancer agents, drug concentration, impedance measurement, field monitoring, cell death
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