Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw
Open accessAbstract
The aim of the current study was to investigate the effects of a lactic acid bacteria inoculant (Lactobacillus plantarum, LP), fibrolytic enzyme (EN), combination of LP and EN (LP+EN) on fermentation quality, nutritive characteristics and in vitro digestibility of total mixed ration (TMR) silages containing 0, 7.5 and 15.0% (on dry matter basis) of rape (Brassica campestris L.) straw (RS) (denoted as CTMR, LTMR and MTMR, respectively). After ensiling for 60 days, TMR silages without additives were well preserved, but MTMR had higher pH than CTMR and LTMR. There were no differences in other parameters of fermentation quality, microbial composition, nutrition and in vitro digestibility between CTMR and LTMR except for yeast and mold number and crude protein (CP) content. CTMR and LTMR silage had higher CP content, in vitro neutral detergent fiber digestibility (IVNDFD) and in vitro acid detergent fiber digestibility (IVADFD), lower acid detergent fiber (ADF) content than MTMR silage. LP and EN decreased pH and increased dry matter (DM) recovery of TMR silages. LP+EN improved the fermentation quality, nutritive characteristics and in vitro digestibility of TMR silages, showed by lower pH, ADF content, higher lactic acid content, in vitro dry matter digestibility (IVDMD) and IVNDFD. Therefore, It was suggested that TMR silage contained 7.5% RS on a DM basis and treated with LP+EN can be as a useful feed for ruminant.
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Liu et al. found that the addition of Lactobacillus casei and cellulase to TMR silage resulted in a lactic acid yield of 3.8% (DM).
“Liu et al. evaluated the effect of additives on the fermentation quality of TMR silage and found that the addition of Lactobacillus casei and cellulase to TMR silage resulted in a lactic acid yield of 3.8% (DM)”
From Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover (2021) · cites this paper as [39]
Table 3 reports a mean lactic acid content of 38.1 g/kg DM (3.81%) for TMR silage treated with the bacterial inoculant and enzyme. But §2.1 identifies the bacterium as Lactobacillus plantarum, not Lactobacillus casei; the enzyme had cellulase activity. The reported yield is supported, but the named species is not. (LLM, conf=0.94)
Experimental — accuracy not yet measuredThe lactic acid content produced by the addition of LAB and CE blend silage in this experiment was 4.12% (DM).
“which was similar to the lactic acid content (4.12%, DM) produced by the addition of LAB and CE blend silage in this experiment [39].”
From Cellulase Interacts with Lactic Acid Bacteria to Affect Fermentation Quality, Microbial Community, and Ruminal Degradability in Mixed Silage of Soybean Residue and Corn Stover (2021) · cites this paper as [39]
Table 3 (listed under Conclusion; page unavailable) reports lactic acid for the combined LAB and enzyme treatment, but not 4.12% of dry matter. Its treatment mean is 38.1 g/kg DM (3.81%); the highest formulation-specific value is 40.8 g/kg DM (4.08%). (LLM, conf=0.90)
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- Authors
- LIU, Q.-H., LI, X.-Y., Desta, S. T., ZHANG, J.-G., & SHAO, T.
- Affiliations
- Nanjing Agricultural University (CN)
- Corresponding author
- SHAO, Tao
- Venue
- Journal of Integrative Agriculture
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 15
- Issue
- 9
- Pages
- 2087-2096
- ISSN
- 2095-3119 (ISSN-L)
- OpenAlex
- W2513140998
- Cited by
- 54
- References
- 38
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 7 · 2023: 9 · 2022: 5 · 2021: 9 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 1
- FWCI
- 6.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Integrative Agriculture (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31502014)
- Government of Jiangsu Province
- Specialized Research Fund for the Doctoral Program of Higher Education of China (20130097120053)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- Lactobacillus plantarum, fibrolytic enzymes, total mixed ration silage, rape straw, silage fermentation quality, in vitro digestibility, lactic acid bacteria inoculant, dry matter recovery, ruminant feed, crude protein, fiber digestibility
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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