Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro
Abstract
Silicon deficiency in animals leads to bone defects. This element may therefore play an important role in bone metabolism. Silicon is absorbed from the diet as orthosilicic acid and concentrations in plasma are 5-20 microM. The in vitro effects of orthosilicic acid (0-50 microM) on collagen type 1 synthesis was investigated using the human osteosarcoma cell line (MG-63), primary osteoblast-like cells derived from human bone marrow stromal cells, and an immortalized human early osteoblastic cell line (HCC1). Collagen type 1 mRNA expression and prolyl hydroxylase activity were also determined in the MG-63 cells. Alkaline phosphatase and osteocalcin (osteoblastic differentiation) were assessed both at the protein and the mRNA level in MG-63 cells treated with orthosilicic acid. Collagen type 1 synthesis increased in all treated cells at orthosilicic acid concentrations of 10 and 20 microM, although the effects were more marked in the clonal cell lines (MG-63, HCCl 1.75- and 1.8-fold, respectively, P < 0.001, compared to 1.45-fold in the primary cell lines). Treatment at 50 microM resulted in a smaller increase in collagen type 1 synthesis (MG-63 1.45-fold, P = 0.004). The effect of orthosilicic acid was abolished in the presence of prolyl hydroxylase inhibitors. No change in collagen type 1 mRNA level was seen in treated MG-63 cells. Alkaline phosphatase activity and osteocalcin were significantly increased (1.5, 1.2-fold at concentrations of 10 and 20 microM, respectively, P < 0.05). Gene expression of alkaline phosphatase and osteocalcin also increased significantly following treatment. In conclusion, orthosilicic acid at physiological concentrations stimulates collagen type 1 synthesis in human osteoblast-like cells and enhances osteoblastic differentiation.
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Orthosilicic acid stimulates fibroblasts to secrete collagen type I.
“Orthosilicic acid (OSA) stimulates fibroblasts to secrete collagen type I [140].”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [140]
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“In general terms, there appe r to be two distinct steps involved in bio istri ution of dietary silicon after oral bsorption: first, the rapid urinary exc etion for most o the ingested silicon [9,15,25]; the other step would consist of tissue storage and/or metabolism for he minor part of the ingested Si, proportional…”
Background
- Authors
- Reffitt, D. M., Ogston, N., Jugdaohsingh, R., Cheung, H. F. J., Evans, B. A. J., Thompson, R. P. H., Powell, J. J., & Hampson, G. N.
- Affiliations
- St Thomas' Hospital (GB)
- Cardiff University (GB)
- Corresponding author
- Hampson, G.N
- Venue
- Bone
- Publisher
- Elsevier BV
- Published
- 2003-02
- Volume
- 32
- Issue
- 2
- Pages
- 127-135
- ISSN
- 1873-2763 (ISSN-L)
- 8756-3282
- OpenAlex
- W2165221289
- Cited by
- 876
- References
- 38
- Citations by year
- 2026: 16 · 2025: 33 · 2024: 47 · 2023: 46 · 2022: 46 · 2021: 54 · 2020: 61 · 2019: 35 · 2018: 48 · 2017: 59 · 2016: 55 · 2015: 48 · 2014: 51 · 2013: 41 · 2012: 38
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bone (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Research Portal (King's College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Silicon Effects in Agriculture
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- orthosilicic acid, silicon, osteogenic differentiation, osteoblast-like cells, MG-63 cells, prolyl hydroxylase, alkaline phosphatase, osteocalcin, bone metabolism, mesenchymal stem cells
- MeSH terms
- Adolescent, Cell Differentiation, Cells, Cultured, Child, Child, Preschool, Dose-Response Relationship, Drug, Female, Humans, Male, Osteoblasts, Silicic Acid, Tumor Cells, Cultured, Stromal Cells, Gene Expression Regulation, Developmental, Collagen Type I
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