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Dietary n-3 fatty acids affect mRNA level of brown adipose tissue uncoupling protein 1, and white adipose tissue leptin and glucose transporter 4 in the rat
Open accessAbstract
We examined the effect of dietary fats rich inn-3 polyunsaturated fatty acids (PUFA) on mRNA levels in white and brown adipose tissues in rats. Four groups of rats were fed on a low-fat diet (20 g safflower oil/kg) or a high-fat diet (200 g/kg) containing safflower oil, which is rich inn-6 PUFA (linoleic acid), or perilla (α-linolenic acid) or fish oil (eicosapentaenoic and docosahexaenoic acids), both of which are rich inn-3 PUFA, for 21 d. Energy intake was higher in rats fed on a high-safflower-oil diet than in those fed on low-fat or high-fish-oil diet, but no other significant differences were detected among the groups. Perirenal white adipose tissue weight was higher and epididymal white adipose tissue weight tended to be higher in rats fed on a high-safflower-oil diet than in those fed on a low-fat diet. However, high-fat diets rich inn-3 PUFA, compared to a low-fat diet, did not increase the white adipose tissue mass. High-fat diets relative to a low-fat diet increased brown adipose tissue uncoupling protein 1 mRNA level. The increases were greater with fats rich inn-3 PUFA than withn-6 PUFA. A high-safflower-oil diet, compared to a low-fat diet, doubled the leptin mRNA level in white adipose tissue. However, high-fat diets rich inn-3 PUFA failed to increase it. Compared to a low-fat diet, high-fat diets down-regulated the glucose transporter 4 mRNA level in white adipose tissue. However, the decreases were attenuated with high-fat diets rich inn-3 PUFA. It is suggested that the alterations in gene expression in adipose tissue contribute to the physiological activities ofn-3 PUFA in preventing body fat accumulation and in regulating glucose metabolism in rats.
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“PUFAs such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) also stimulate UCP1 expression and thus adaptive non-shivering or diet-induced thermogenesis in brown adipocytes, as well as the browning of the white adipose tissue (WAT) in C57BL/6 mice [67,68], Sprague-Dawley rats [69,70], and overweight humans [71].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [70]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
Positional cloning of the mouse obese gene and its human homologue
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., & Friedman, J. M. (1994) · Nature · doi:10.1038/372425a0 · Cited by 13,419
Report of the American Institute of Nutrition Ad Hoc Committee on Standards for Nutritional Studies
(1977) · Journal of Nutrition · doi:10.1093/jn/107.7.1340 · Cited by 2,867
Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia
Fleury, C., Neverova, M., Collins, S., Raimbault, S., Champigny, O., Lévi-Meyrueis, C., Bouillaud, F., Seldin, M. F., Surwit, R. S., Ricquier, D., et al. (1997) · Nature Genetics · doi:10.1038/ng0397-269 · Cited by 1,630
Thermogenic mechanisms in brown fat.
Nicholls, D. G., & Locke, R. M. (1984) · Physiological Reviews · doi:10.1152/physrev.1984.64.1.1 · Cited by 1,602 · cited 1×
“Mitochondrial protein called UCP 1, which is solely expressed in brown adipose tissue, induces non-shivering thermogenesis by creating a pathway that allows dissipation of the proton electrochemical membrane (Nicholls & Locke, 1984).”
BackgroundMolecular cloning and characterization of an insulin-regulatable glucose transporter
James, D. E., Strube, M., & Muecdler, M. (1989) · Nature · doi:10.1038/338083a0 · Cited by 901
Gong, D., He, Y., Karas, M., & Reitman, M. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.39.24129 · Cited by 740
The OB Protein (Leptin) Pathway - A Link Between Adipose Tissue Mass and Central Neural Networks
Campfield, L. A., Smith, F., & Burn, P. (1996) · Hormone and Metabolic Research · doi:10.1055/s-2007-979867 · Cited by 501
Brown adipose tissue thermogenesis: interdisciplinary studies
Himms‐Hagen, J. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.11.2199286 · Cited by 462
Jacobsson, A., Stadler, U., Glotzer, M. A., & Kozak, L. P. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36228-2 · Cited by 300
The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family.
Kirchgessner, T. G., Svenson, K. L., Lusis, A. J., & Schotz, M. C. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47435-2 · Cited by 297
ADIPOCYTE DIFFERENTIATION AND LEPTIN EXPRESSION
Hwang, C.-S., Loftus, T. M., Mandrup, S., & Lane, M. D. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.231 · Cited by 249
Bouillaud, F., Ricquier, D., Thibault, J., & Weissenbach, J. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.445 · Cited by 244
Ricquier, D., Bouillaud, F., Toumelin, P. L., Mory, G., Bazin, R., Arch, J. R. S., & Pénicaud, L. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66957-1 · Cited by 234
Complete cDNA-derived amino acid sequence of rat brown fat uncoupling protein.
Bouillaud, F., Weissenbach, J., & Ricquier, D. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35962-8 · Cited by 222
Raclot, T., Groscolas, R., Langin, D., & Ferré, P. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37127-3 · Cited by 215
Ogawa, Y., Masuzaki, H., Isse, N., Okazaki, T., Mori, K., Shigemoto, M., Satoh, N., Tamura, N., Hosoda, K., & Yoshimasa, Y. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118204 · Cited by 214
Belzung, F., Raclot, T., & Groscolas, R. (1993) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1993.264.6.r1111 · Cited by 193
Matsuda, J., Hosoda, K., Itoh, H., Son, C., Doi, K., Tanaka, T., Fukunaga, Y., Inoue, G., Nishimura, H., Yoshimasa, Y., et al. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01381-1 · Cited by 186
Semenkovich, C. F., Chen, S. H., Wims, M., Luo, C. C., Li, W. H., & Chan, L. (1989) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38369-3 · Cited by 160
Okuno, M., Kajiwara, K., Imai, S., Kobayashi, T., Honma, N., Maki, T., Suruga, K., Goda, T., Takase, S., Muto, Y., et al. (1997) · Journal of Nutrition · doi:10.1093/jn/127.9.1752 · Cited by 154
Regulation by Dietary Fats of 3-Hydroxy-3-Methylglutaryl-Coenzyme a Reductase in Rat Liver
Ide, T., OKAMATSU, H., & Sugano, M. (1978) · Journal of Nutrition · doi:10.1093/jn/108.4.601 · Cited by 147
Self-citationMercer, S. W., & Trayhurn, P. (1987) · The Journal of Nutrition · doi:10.1093/jn/117.12.2147 · Cited by 146
Pedersen, O. B., Kahn, C. R., Flier, J. S., & Kahn, B. B. (1991) · Endocrinology · doi:10.1210/endo-129-2-771 · Cited by 119
Luo, J., Rizkalla, S. W., Boillot, J., Alamowitch, C., Chaı̈b, H., Bruzzo, F., Desplanque, N., Dalix, A. M., Durand, G., & Slama, G. (1996) · Journal of Nutrition · doi:10.1093/jn/126.8.1951 · Cited by 113
Brown fat thermogenesis in rats fed high-fat diets enriched with n-3 polyunsaturated fatty acids
Oudart, H., Groscolas, R., Calgari, C., Nibbelink, M., Leray, C., Le Maho, Y., & Malan, A. (1997) · International Journal of Obesity · doi:10.1038/sj.ijo.0800500 · Cited by 110
Ramsay, T. G. (1996) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/s0889-8529(05)70358-3 · Cited by 97
Rapid increase of mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue
Ricquier, D., Mory, G., Bouillaud, F., Thibai, J., & Weissenbach, J. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)80608-0 · Cited by 96
Plasma Leptin Levels and Body Fat Distribution
Takahashi, M., Funahashi, T., Shimomura, I., Miyuoka, K., & Matsuzawa, Y. (1996) · Hormone and Metabolic Research · doi:10.1055/s-2007-979893 · Cited by 94
Augmented Expression of the obese Gene in the Adipose Tissue from Rats Fed High-Fat Diet
Masuzaki, H., Ogawa, Y., Hosoda, K., Kawada, T., Fushiki, T., & Nakao, K. (1995) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1995.2631 · Cited by 86
Giraudo, S. Q., Kotz, C. M., Grace, M. K., Levine, A. S., & Billington, C. J. (1994) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1994.266.5.r1578 · Cited by 84
Pharmacologic Manipulation of ob Expression in a Dietary Model of Obesity
Collins, S., & Surwit, R. S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.16.9437 · Cited by 81
Huang, Y. J., Fang, V. S., Juan, C., Chou, Y.-C., Kwok, C., & Ho, L. T. (1997) · Metabolism · doi:10.1016/s0026-0495(97)90226-2 · Cited by 77
Kahn, B. B. (1994) · Journal of Nutrition · doi:10.1093/jn/124.suppl_8.1289s · Cited by 69
Developmental and nutritional changes of ob and PPARγ2 gene expression in rat white adipose tissue
Rousseau, V., Becker, D., ONGEMBA, L. N., Rahier, J., Henquin, J., & Brichard, S. M. (1997) · Biochemical Journal · doi:10.1042/bj3210451 · Cited by 56
Effects of a fish oil-lard diet on rat plasma lipoproteins, liver FAS, and lipolytic enzymes
Benhizia, F., Hainault, I., Sérougne, C., Lagrange, D., Hajduch, É., Guichard, C., Malewiak, M. I., Quignard‐Boulangé, A., Lavau, M. M., & Griglio, S. (1994) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1994.267.6.e975 · Cited by 47
Sequence of rat lipoprotein lipase-encoding cDNA
Brault, D., Noé, L., Etienne, J., Hamelin, J., Raisonnier, A., Souli, A., Chuat, J., Dugail, I., Quignard‐Boulangé, A., Lavau, M. M., et al. (1992) · Gene · doi:10.1016/0378-1119(92)90127-b · Cited by 35
Kawada, T., Kayahashi, S., Hida, Y., Koga, K.-J., Nadachi, Y., & Fushiki, T. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9711000 · Cited by 34
Nelson, G. J., Kelley, D. S., Schmidt, P. C., & Serrato, C. M. (1987) · Lipids · doi:10.1007/bf02534003 · Cited by 33
Sevilla, L., Gumà, A., Enrique-Tarancón, G., Mora, S., Muñoz, P., Palacı́n, M., Testar, X., & Zorzano, A. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6742 · Cited by 31
Ezaki, O., Tsuji, E., Momomura, K., Kasuga, M., & Itakura, H. (1992) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1992.263.1.e94 · Cited by 29
Increase of uncoupling protein and its mRNA in brown adipose tissue of rats fed on ‘cafeteria diet’
Falcou, R., Bouillaud, F., Mory, G., Apfelbaum, M., & Ricquier, D. (1985) · Biochemical Journal · doi:10.1042/bj2310241 · Cited by 26
Martin, I., Giralt, M., Viñas, O., Iglesias, R., Mampel, T., & Villarroya, F. (1995) · Biochemical Journal · doi:10.1042/bj3080749 · Cited by 20
Ide, T., Kano, S., Murata, M., Yanagita, T., & SUGANO, M. (1994) · British Journal Of Nutrition · doi:10.1079/bjn19940012 · Cited by 19
Self-citationMiura, T., Ohnishi, Y., TAKAGI, S., Sawamura, M., YASUDA, N., Ishida, H., Tanigawa, K., Yamori, Y., & Seino, Y. (1997) · Journal of Nutritional Science and Vitaminology · doi:10.3177/jnsv.43.225 · Cited by 12
Leptin — fat messenger or fat controller?
Qureshi, A., & Kopelman, P. G. (1997) · Clinical Endocrinology · doi:10.1046/j.1365-2265.1997.2931092.x · Cited by 11
Research works printed but not linked: 2
- Snedecor, 1989, Statistical Methods
- 10.1016/0003-2697(87)90021-2 · DOI
Cited by
Sorted by citations
Brown Adipose Tissue: Function and Physiological Significance
CANNON, B., & NEDERGAARD, J. (2004) · Physiological Reviews · doi:10.1152/physrev.00015.2003 · Cited by 6,433
Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases
Demine, S., Renard, P., & Arnould, T. (2019) · Cells · doi:10.3390/cells8080795 · Cited by 476
“PUFAs such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) also stimulate UCP1 expression and thus adaptive non-shivering or diet-induced thermogenesis in brown adipocytes, as well as the browning of the white adipose tissue (WAT) in C57BL/6 mice [67,68], Sprague-Dawley rats [69,70], and overweight human…”
BackgroundFlachs, P., Horakova, O., Brauner, P., Rossmeisl, M., Pecina, P., Franssen-van Hal, N., Ruzickova, J., Sponarova, J., Drahota, Z., Vlcek, C., et al. (2005) · Diabetologia · doi:10.1007/s00125-005-1944-7 · Cited by 388
Omega-3 fatty acids and metabolic syndrome: Effects and emerging mechanisms of action
Poudyal, H., Panchal, S. K., Diwan, V., & Brown, L. (2011) · Progress in Lipid Research · doi:10.1016/j.plipres.2011.06.003 · Cited by 391
Lombardo, Y. B., & Chicco, A. G. (2005) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2005.08.002 · Cited by 364
Metadata
- Authors
- Takahashi, Y., & Ide, T.
- Affiliations
- Ministry of Agriculture, Forestry and Fisheries (JP)
- Corresponding author
- Ide, Takashi
- Venue
- British Journal of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2000-08
- Volume
- 84
- Issue
- 2
- Pages
- 175-184
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2166122956
- Cited by
- 175
- References
- 50
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 9 · 2019: 11 · 2018: 8 · 2017: 9 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 10 · 2012: 7
- FWCI
- 7.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- omega-3 fatty acids, brown adipose tissue, white adipose tissue, UCP1, leptin, glucose transporter 4, perilla oil, fish oil, safflower oil, dietary fat, adipose tissue gene expression, body fat accumulation, glucose metabolism, energy intake
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