A Glucose Biosensor Based on Surface-Enhanced Raman Scattering: Improved Partition Layer, Temporal Stability, Reversibility, and Resistance to Serum Protein Interference
Abstract
This work updates the recent progress made toward fabricating a real-time, quantitative, and biocompatible glucose sensor based on surface-enhanced Raman scattering (SERS). The sensor design relies on an alkanethiolate tri(ethylene glycol) monolayer that acts as a partition layer, preconcentrating glucose near a SERS-active surface. Chemometric analysis of the captured SERS spectra demonstrates that glucose is quantitatively detected in the physiological concentration range (0-450 mg/dL, 0-25 mM). In fact, 94% of the predicted glucose concentrations fall within regions A and B of the Clarke error grid, making acceptable predictions in a clinically relevant range. The data presented herein also demonstrate that the glucose sensor provides stable SERS spectra for at least 3 days, making the SERS substrate a candidate for implantable sensing. Glucose sensor reversibility and reusability is evaluated as the sensor is alternately exposed to glucose and saline solutions; after each cycle, difference spectra reveal that the partitioning process is largely reversible. Finally, the SERS glucose sensor successfully partitions glucose even when challenged with bovine serum albumin, a serum protein mimic.
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Surface enhanced Raman spectroscopy dramatically shortens acquisition times and improves sensitivity and limit of detection.
“Recent advances have demonstrated that surface enhanced Raman spectroscopy (SERS) dramatically decreases acquisition times with concomitant improvements in both sensitivity and limit of detection.45–46”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [46]
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Biocompatible Materials for Continuous Glucose Monitoring Devices
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- Authors
- Yonzon, C. R., Haynes, C. L., Zhang, X., Walsh, & Van Duyne, R. P.
- Affiliations
- Northwestern University (US)
- Department of Chemistry and Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-11-25
- Volume
- 76
- Issue
- 1
- Pages
- 78-85
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2014133708
- Cited by
- 376
- References
- 36
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 14 · 2022: 9 · 2021: 8 · 2020: 15 · 2019: 12 · 2018: 13 · 2017: 22 · 2016: 12 · 2015: 19 · 2014: 21 · 2013: 26 · 2012: 25
- FWCI
- 13.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Northwestern University
- National Institutes of Health (5r01ey013002-03)
- Multidisciplinary University Research Initiative
- National Eye Institute (EY13015, R24EY013015, EY13002)
- Air Force Office of Scientific Research
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Keywords
- glucose biosensor, surface-enhanced Raman scattering, bovine serum albumin, sensor reversibility, sensor reusability, temporal stability, chemometric analysis, Clarke error grid
- MeSH terms
- Animals, Blood Proteins, Cattle, Drug Stability, Glucose, Spectrum Analysis, Raman, Time Factors, Biosensing Techniques
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