The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Microfluidic Single-Cell mRNA Isolation and Analysis
Abstract
Single-cell gene expression analysis holds great promise for studying diverse biological systems, but methodology to process these precious samples in a reproducible, quantitative, and parallel fashion remains challenging. Here, we utilize microfluidics to isolate picogram and subpicogram mRNA templates, as well as to synthesize cDNA from these templates. We demonstrate single-cell mRNA isolation and cDNA synthesis, provide quantitative calibrations for each step in the process, and measure gene expression in individual cells. The techniques presented here form the foundation for highly parallel single-cell gene expression studies.
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Cites
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Single-Cell Gene Expression Profiling
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Cited by
Regev, A., Teichmann, S. A., Lander, E. S., Amit, I., Benoist, C., Birney, E., Bodenmiller, B., Campbell, P., Carninci, P., Clatworthy, M., et al. (2017) · eLife · doi:10.7554/elife.27041 · Cited by 2,232
Self-citationMicrofluidic lab-on-a-chip platforms: requirements, characteristics and applications
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Habermann, A. C., Gutierrez, A. J., Bui, L. T., Yahn, S. L., Winters, N. I., Calvi, C. L., Peter, L., Chung, M.-I., Taylor, C. J., Jetter, C., et al. (2020) · Science Advances · doi:10.1126/sciadv.aba1972 · Cited by 1,129
Buettner, F., Natarajan, K. N., Casale, F. P., Proserpio, V., Scialdone, A., Theis, F. J., Teichmann, S. A., Marioni, J. C., & Stegle, O. (2015) · Nature Biotechnology · doi:10.1038/nbt.3102 · Cited by 1,136
Microfluidic Devices for Bioapplications
Yeo, L. Y., Chang, H., Chan, P. P. Y., & Friend, J. R. (2011) · Small · doi:10.1002/smll.201000946 · Cited by 622
Metadata
- Authors
- Marcus, J. S., Anderson, W. F., & Quake, S. R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-03-31
- Volume
- 78
- Issue
- 9
- Pages
- 3084-3089
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2060929285
- Cited by
- 351
- References
- 19
- FWCI
- 15.84
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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