Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

Habermann, Arun C.; Gutierrez, Austin J.; Bui, Linh T.; Yahn, Stephanie L.; Winters, Nichelle I.; Calvi, Carla L.; Peter, Lance; Chung, Mei-I; Taylor, Chase J.; Jetter, Christopher; Raju, Latha; Roberson, Jamie; Ding, Guixiao; Wood, Lori; Sucre, Jennifer M. S.; Richmond, Bradley W.; Serezani, Ana P.; McDonnell, Wyatt J.; Mallal, Simon B.; Bacchetta, Matthew J.; Loyd, James E.; Shaver, Ciara M.; Ware, Lorraine B.; Bremner, Ross; Walia, Rajat; Blackwell, Timothy S.; Banovich, Nicholas E.; Kropski, Jonathan A.

doi:10.1126/sciadv.aba1972

Cited by 1,129Interstitial Lung Diseases and Idiopathic Pulmonary FibrosisSingle-cell and spatial transcriptomicsTransplantation: Methods and Outcomes

Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

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Habermann, Arun C., Gutierrez, Austin J., Bui, Linh T., Yahn, Stephanie L., Winters, Nichelle I., Calvi, Carla L., Peter, Lance, Chung, Mei-I +20 more·2020·Science Advances

DOI 10.1126/sciadv.aba1972PMC PMC7439444

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Abstract

Single-cell RNA sequencing provides new insights into pathologic epithelial and mesenchymal remodeling in the human lung.

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Profibrotic macrophage populations dominate the immune cell landscape in IPF.
“In terms of the IPF immune cell compartment, profibrotic macrophage populations dominate the immune cell landscape in IPF (Adams et al., 2020; Habermann et al., 2020).”
From Pulmonary fibrosis: Emerging diagnostic and therapeutic strategies (2023) · cites this paper as Habermann et al., 2020

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Authors: Habermann, A. C., Gutierrez, A. J., Bui, L. T., Yahn, S. L., Winters, N. I., Calvi, C. L., Peter, L., Chung, M.-I., Taylor, C. J., Jetter, C., Raju, L., Roberson, J., Ding, G., Wood, L., Sucre, J. M. S., Richmond, B. W., Serezani, A. P., McDonnell, W. J., Mallal, S. B., Bacchetta, M. J., Loyd, J. E., Shaver, C. M., Ware, L. B., Bremner, R., Walia, R., Blackwell, T. S., Banovich, N. E., & Kropski, J. A.
Affiliations: Vanderbilt University Medical Center (US)
Translational Genomics Research Institute (US)
Department of Thoracic Disease and Transplantation, Norton Thoracic Institute, Phoenix, AZ, USA.
Nashville VA Medical Center (US)
Vanderbilt University (US)
Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Corresponding author: Banovich, Nicholas E.
Kropski, Jonathan A.
ORCID: Habermann, Arun C.
Gutierrez, Austin J.
Bui, Linh T.
Yahn, Stephanie L.
Peter, Lance
Taylor, Chase J.
Jetter, Christopher
Raju, Latha
Wood, Lori
Sucre, Jennifer M. S.
Richmond, Bradley W.
Serezani, Ana P.
McDonnell, Wyatt J.
Loyd, James E.
Shaver, Ciara M.
Ware, Lorraine B.
Bremner, Ross
Walia, Rajat
Blackwell, Timothy S.
Banovich, Nicholas E.
Kropski, Jonathan A.
Venue: Science Advances
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2020-07-10
Volume: 6
Issue: 28
Pages: eaba1972-eaba1972
ISSN: 2375-2548 (ISSN-L)
DOI: 10.1126/sciadv.aba1972
PMC: PMC7439444
OpenAlex: W3040876684
Cited by: 1,129
References: 65
Citations by year: 2026: 195 · 2025: 211 · 2024: 196 · 2023: 177 · 2022: 165 · 2021: 120 · 2020: 60 · 2019: 3
FWCI: 73.47
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by-nc
creativecommons.org/licenses/by-nc/4.0/ (vor)
Locations: Science Advances (publishedVersion, cc-by-nc)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
PubMed Central (publishedVersion, cc-by-nc)
PubMed (publishedVersion)
Murdoch University Research Portal (submittedVersion)
Funders: National Heart, Lung, and Blood Institute (K08HL130595, P01HL92870, R01HL145372)
Doris Duke Charitable Foundation
Topics: Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
Health Sciences › Medicine › Surgery › Transplantation: Methods and Outcomes
Keywords: pulmonary fibrosis, fibroblast subtypes, extracellular matrix expansion, distal lung epithelium, epithelial-mesenchymal plasticity, single-cell transcriptomics, lung fibrosis pathogenesis
MeSH terms: Extracellular Matrix, Fibrosis, Humans, Lung, Pulmonary Fibrosis, Sequence Analysis, RNA

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Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis

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