The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Aptamer-Based Electrochemical Biosensor for Interferon Gamma Detection
Open accessAbstract
In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.
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Cites
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Functional Nucleic Acid Sensors
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Cited by
Kartikasari, A. E. R., Huertas, C. S., Mitchell, A., & Plebanski, M. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.692142 · Cited by 343
Bioanalytical Chemistry of Cytokines–a Review
Stenken, J. A., & Poschenrieder, A. J. (2015) · Analytica Chimica Acta · doi:10.1016/j.aca.2014.10.009 · Cited by 302
Screening of a ScFv Antibody With High Affinity for Application in Human IFN-γ Immunoassay
Yang, H., Zhong, Y., Wang, J., Zhang, Q., Li, X., Ling, S., Wang, S., & Wang, R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00261 · Cited by 25
Metadata
- Authors
- Liu, Y., Tuleouva, N., Ramanculov, E., & Revzin, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-09-03
- Volume
- 82
- Issue
- 19
- Pages
- 8131-8136
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2058239128
- Cited by
- 324
- References
- 29
- FWCI
- 7.68
- Type
- journal article
- Language
- en
- Open access
- green
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