The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Aptamer-Based Electrochemical Biosensor for Interferon Gamma Detection
Open accessAbstract
In this paper, we describe the development of an electrochemical DNA aptamer-based biosensor for detection of interferon (IFN)-γ. A DNA hairpin containing IFN-γ-binding aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. Binding of IFN-γ caused the aptamer hairpin to unfold, pushing MB redox molecules away from the electrode and decreasing electron-transfer efficiency. The change in redox current was quantified using square wave voltammetry (SWV) and was found to be highly sensitive to IFN-γ concentration. The limit of detection for optimized biosensor was 0.06 nM with linear response extending to 10 nM. This aptasensor was specific to IFN-γ in the presence of overabundant serum proteins. Importantly, the same aptasensor could be regenerated by disrupting aptamer-IFN-γ complex in urea buffer and reused multiple times. Unlike standard sandwich immunoassays, the aptasensor described here allowed one to detect IFN-γ binding directly without the need for multiple washing steps and reagents. An electrochemical biosensor for simple and sensitive detection of IFN-γ demonstrated in this paper will have future applications in immunology, cancer research, and infectious disease monitoring.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
In vitro selection of RNA molecules that bind specific ligands
Ellington, A. D., & Szostak, J. W. (1990) · Nature · doi:10.1038/346818a0 · Cited by 9,812
CELLULAR RESPONSES TO INTERFERON-γ
Boehm, U., Klamp, T., Groot, M., & Howard, J. C. (1997) · Annual Review of Immunology · doi:10.1146/annurev.immunol.15.1.749 · Cited by 2,890
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
Flynn, J. L., Chan, J., Triebold, K. J., Dalton, D. K., Stewart, T. A., & Bloom, B. R. (1993) · The Journal of experimental medicine · doi:10.1084/jem.178.6.2249 · Cited by 2,465
Drummond, T. G., Hill, M. G., & Barton, J. K. (2003) · Nature Biotechnology · doi:10.1038/nbt873 · Cited by 2,214
Functional Nucleic Acid Sensors
Liu, J., Cao, Z., & Lu, Y. (2009) · Chemical Reviews · doi:10.1021/cr030183i · Cited by 2,187
Characterization of DNA Probes Immobilized on Gold Surfaces
Herne, T. M., & Tarlov, M. J. (1997) · Journal of the American Chemical Society · doi:10.1021/ja9719586 · Cited by 1,541
Song, S., Wang, L., Li, J., Fan, C., & Zhao, J. (2008) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2007.12.004 · Cited by 1,434
Electrochemical Quantitation of DNA Immobilized on Gold
Steel, A. B., Herne, T. M., & Tarlov, M. J. (1998) · Analytical Chemistry · doi:10.1021/ac980037q · Cited by 1,328
Target-Responsive Structural Switching for Nucleic Acid-Based Sensors
Li, D., Song, S., & Fan, C. (2010) · Accounts of Chemical Research · doi:10.1021/ar900245u · Cited by 737
Fan, R., Vermesh, O., Srivastava, A., Yen, B. K. H., Qin, L., Ahmad, H., Kwong, G. A., Liu, C.-C., Gould, J., Hood, L., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt.1507 · Cited by 539
Folding-Based Electrochemical Biosensors: The Case for Responsive Nucleic Acid Architectures
Lubin, A. A., & Plaxco, K. W. (2010) · Accounts of Chemical Research · doi:10.1021/ar900165x · Cited by 506
Xiao, Y., Lai, R. Y., & Plaxco, K. W. (2007) · Nature Protocols · doi:10.1038/nprot.2007.413 · Cited by 439
White, R. J., Phares, N., Lubin, A. A., Xiao, Y., & Plaxco, K. W. (2008) · Langmuir · doi:10.1021/la800801v · Cited by 358
Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor
Ricci, F., Lai, R. Y., Heeger, A. J., Plaxco, K. W., & Sumner, J. J. (2007) · Langmuir · doi:10.1021/la700328r · Cited by 334
Reece, W. H. H., Pinder, M., Gothard, P. K., Milligan, P., Bojang, K., Doherty, T., Plebanski, M., Akinwunmi, P., Everaere, S., Watkins, K. R., et al. (2004) · Nature Medicine · doi:10.1038/nm1009 · Cited by 264
Karlsson, A. C., Martin, J. N., Younger, S. R., Bredt, B. M., Epling, L., Ronquillo, R., Varma, A., Deeks, S. G., McCune, J. M., Nixon, D. F., et al. (2003) · Journal of Immunological Methods · doi:10.1016/j.jim.2003.09.001 · Cited by 223
Sassolas, A., Blum, L. J., & Leca‐Bouvier, B. D. (2009) · Electroanalysis · doi:10.1002/elan.200804554 · Cited by 173
An Evaluation of Commercial Fluorescent Bead-Based Luminex Cytokine Assays
Djoba Siawaya, J. F., Roberts, T., Babb, C., Black, G., Golakai, H. J., Stanley, K., Bapela, N. B., Hoal, E., Parida, S., van Helden, P., et al. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002535 · Cited by 172
Development of an Aptamer Beacon for Detection of Interferon-Gamma
Tuleuova, N., Jones, C. N., Yan, J., Ramanculov, E. M., Yokobayashi, Y., & Revzin, A. (2010) · Analytical Chemistry · doi:10.1021/ac9025237 · Cited by 146
Self-citationMeasurement of cytokine release at the single cell level using the ELISPOT assay
Cox, J. H., Ferrari, G., & Janetzki, S. (2006) · Methods · doi:10.1016/j.ymeth.2005.11.006 · Cited by 119
A simple and direct electrochemical detection of interferon-γ using its RNA and DNA aptamers
Min, K., Cho, M., Han, S.-Y., Shim, Y., Ku, J., & Ban, C. (2008) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2008.02.021 · Cited by 111
A microdevice for multiplexed detection of T-cell-secreted cytokines
Zhu, H., Stybayeva, G., Macal, M., Ramanculov, E. M., George, M. D., Dandekar, S., & Revzin, A. (2008) · Lab on a Chip · doi:10.1039/b810244a · Cited by 102
Self-citationDetecting Cytokine Release from Single T-cells
Zhu, H., Stybayeva, G., Silangcruz, J., Yan, J., Ramanculov, E. M., Dandekar, S., George, M. D., & Revzin, A. (2009) · Analytical Chemistry · doi:10.1021/ac901390j · Cited by 89
Self-citationArray-Based Multiplexed Screening and Quantitation of Human Cytokines and Chemokines
Wang, C. C., Huang, R., Sommer, M., et al. (2002) · Journal of Proteome Research · doi:10.1021/pr0255203 · Cited by 78
Ricci, F., Bonham, A. J., Mason, A. C., Reich, N. O., & Plaxco, K. W. (2009) · Analytical Chemistry · doi:10.1021/ac802365x · Cited by 72
Herrmann, M., Veres, T., & Tabrizian, M. (2008) · Analytical Chemistry · doi:10.1021/ac800427z · Cited by 47
Yan, J., Sun, Y. H., Zhu, H., Marcu, L., & Revzin, A. (2009) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2009.01.029 · Cited by 42
Self-citationInterferon-γ has immunomodulatory effects with minor endocrine and metabolic effects in humans
de Metz, J., Sprangers, F., Endert, E., Ackermans, M. T., ten Berge, I. J. M., Sauerwein, H. P., & Romijn, J. A. (1999) · Journal of Applied Physiology · doi:10.1152/jappl.1999.86.2.517 · Cited by 30
Simultaneous Analysis of Circulating Human Cytokines Using a High-Sensitivity Cytokine Biochip Array
Fitzgerald, S., McConnell, R. I., & Huxley, A. J. M. (2007) · Journal of Proteome Research · doi:10.1021/pr070409o · Cited by 29
Cited by
Kartikasari, A. E. R., Huertas, C. S., Mitchell, A., & Plebanski, M. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.692142 · Cited by 343
Bioanalytical Chemistry of Cytokines–a Review
Stenken, J. A., & Poschenrieder, A. J. (2015) · Analytica Chimica Acta · doi:10.1016/j.aca.2014.10.009 · Cited by 302
Screening of a ScFv Antibody With High Affinity for Application in Human IFN-γ Immunoassay
Yang, H., Zhong, Y., Wang, J., Zhang, Q., Li, X., Ling, S., Wang, S., & Wang, R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00261 · Cited by 25
Metadata
- Authors
- Liu, Y., Tuleouva, N., Ramanculov, E., & Revzin, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-09-03
- Volume
- 82
- Issue
- 19
- Pages
- 8131-8136
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2058239128
- Cited by
- 324
- References
- 29
- FWCI
- 7.68
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
