The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Positive Potential Operation of a Cathodic Electrogenerated Chemiluminescence Immunosensor Based on Luminol and Graphene for Cancer Biomarker Detection
Open accessAbstract
In this work, we report a cathodic electrogenerated chemiluminescence (ECL) of luminol at a positive potential (ca. 0.05 V vs Ag/AgCl) with a strong light emission on the graphene-modified glass carbon electrode. The resulted graphene-modified electrode offers an excellent platform for high-performance biosensing applications. On the basis of the cathodic ECL signal of luminol on the graphene-modified electrode, an ECL sandwich immunosensor for sensitive detection of cancer biomarkers at low potential was developed with a multiple signal amplification strategy from functionalized graphene and gold nanorods multilabeled with glucose oxidase (GOx) and secondary antibody (Ab(2)). The functionalized graphene improved the electron transfer on the electrode interface and was employed to attach the primary antibody (Ab(1)) due to it large surface area. The gold nanorods were not only used as carriers of secondary antibody (Ab(2)) and GOx but also catalyzed the ECL reaction of luminol, which further amplified the ECL signal of luminol in the presence of glucose and oxygen. The as-proposed low-potential ECL immunosensor exhibited high sensitivity and specificity on the detection of prostate protein antigen (PSA), a biomarker of prostate cancer that was used as a model. A linear relationship between ECL signals and the concentrations of PSA was obtained in the range from 10 pg mL(-1) to 8 ng mL(-1). The detection limit of PSA was 8 pg mL(-1) (signal-to-noise ratio of 3). Moreover, the as-proposed low-potential ECL immunosensor exhibited excellent stability and reproducibility. The graphene-based ECL immunosensor accurately detected PSA concentration in 10 human serum samples from patients demonstrated by excellent correlations with standard chemiluminescence immunoassay. The results suggest that the as-proposed graphene ECL immunosensor will be promising in the point-of-care diagnostics application of clinical screening of cancer biomarkers.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Geim, A. K., & Novoselov, K. S. (2007) · Nature Materials · doi:10.1038/nmat1849 · Cited by 39,979
Preparation of Graphitic Oxide
Hummers, W. S., & Offeman, R. E. (1958) · Journal of the American Chemical Society · doi:10.1021/ja01539a017 · Cited by 29,920
Mass spectrometry-based proteomics
Aebersold, R., & Mann, M. (2003) · Nature · doi:10.1038/nature01511 · Cited by 6,955
Systems biology: a brief overview
Kitano, H. (2002) · Science · doi:10.1126/science.1069492 · Cited by 4,265
Graphene Based Electrochemical Sensors and Biosensors: A Review
Shao, Y., Wang, J., Wu, H., Liu, J., Aksay, I. A., & Lin, Y. (2010) · Electroanalysis · doi:10.1002/elan.200900571 · Cited by 3,124
Electrochemiluminescence (ECL)
Richter, M. M. (2004) · Chemical Reviews · doi:10.1021/cr020373d · Cited by 2,588
Electrogenerated Chemiluminescence and Its Biorelated Applications
Miao, W. (2008) · Chemical Reviews · doi:10.1021/cr068083a · Cited by 2,129
A Graphene Platform for Sensing Biomolecules
Lu, C., Yang, H., Zhu, C., Chen, X., & Chen, G. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200901479 · Cited by 2,051
Electrochemical Sensing and Biosensing Platform Based on Chemically Reduced Graphene Oxide
Zhou, M., Zhai, Y., & Dong, S. (2009) · Analytical Chemistry · doi:10.1021/ac900136z · Cited by 1,706
Carbon Nanomaterials in Biosensors: Should You Use Nanotubes or Graphene?
Yang, W., Ratinac, K. R., Ringer, S. P., Thordarson, P., Gooding, J. J., & Braet, F. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.200903463 · Cited by 1,431
Graphene-based materials in electrochemistry
Chen, D., Tang, L., & Li, J. (2010) · Chemical Society Reviews · doi:10.1039/b923596e · Cited by 1,408
Self-citationSeeded High Yield Synthesis of Short Au Nanorods in Aqueous Solution
Sau, T. K., & Murphy, C. J. (2004) · Langmuir · doi:10.1021/la049463z · Cited by 1,396
Direct Electrochemistry of Glucose Oxidase and Biosensing for Glucose Based on Graphene
Shan, C., Yang, H., Song, J., Han, D., Ivaska, A., & Niu, L. (2009) · Analytical Chemistry · doi:10.1021/ac802193c · Cited by 1,315
Glucose Oxidase–graphene–chitosan modified electrode for direct electrochemistry and glucose sensing
Kang, X., Wang, J., Wu, H., Aksay, I. A., Liu, J., & Lin, Y. (2009) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2009.09.004 · Cited by 1,229
Preparation, Structure, and Electrochemical Properties of Reduced Graphene Sheet Films
Tang, L., Wang, Y., Li, Y., Feng, H., Lu, J., & Li, J. (2009) · Advanced Functional Materials · doi:10.1002/adfm.200900377 · Cited by 1,178
Self-citationApplication of graphene-modified electrode for selective detection of dopamine
Wang, Y., Li, Y., Tang, L., Lu, J., & Li, J. (2009) · Electrochemistry Communications · doi:10.1016/j.elecom.2009.02.013 · Cited by 1,117
Self-citationProstate-specific antigen and prostate cancer: prediction, detection and monitoring
Lilja, H., Ulmert, D., & Vickers, A. J. (2008) · Nature Reviews Cancer · doi:10.1038/nrc2351 · Cited by 921
Organic Photovoltaic Devices Based on a Novel Acceptor Material: Graphene
Liu, Z., Liu, Q., Huang, Y., Ma, Y., Yin, S., Zhang, X., Sun, W., & Chen, Y. (2008) · Advanced Materials · doi:10.1002/adma.200800366 · Cited by 852
Weston, A. D., & Hood, L. (2004) · Journal of Proteome Research · doi:10.1021/pr0499693 · Cited by 731
Enzyme immunoassays with special reference to ELISA techniques.
Voller, A., Bartlett, A., & Bidwell, D. E. (1978) · Journal of Clinical Pathology · doi:10.1136/jcp.31.6.507 · Cited by 700
Electrogenerated Chemiluminescence
(2004) · CRC Press eBooks · doi:10.1201/9780203027011 · Cited by 687
Du, D., Zou, Z., Shin, Y., Wang, J., Wu, H., Engelhard, M. H., Liu, J., Aksay, I. A., & Lin, Y. (2010) · Analytical Chemistry · doi:10.1021/ac100036p · Cited by 448
Liao, H., & Hafner, J. H. (2005) · Chemistry of Materials · doi:10.1021/cm050935k · Cited by 434
Bertoncello, P., & Forster, R. J. (2009) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2009.02.013 · Cited by 336
Du, D., Wang, L., Shao, Y., Wang, J., Engelhard, M. H., & Lin, Y. (2011) · Analytical Chemistry · doi:10.1021/ac101715s · Cited by 306
Electrochemiluminescence Immunosensor Based on CdSe Nanocomposites
Jie, G., Zhang, J., Wang, D., Cheng, C., Chen, H.-Y., & Zhu, J.-J. (2008) · Analytical Chemistry · doi:10.1021/ac800052g · Cited by 268
High-sensitivity miniaturized immunoassays for tumor necrosis factor ? using microfluidic systems
Cesaro-Tadic, S., Dernick, G., Juncker, D., Buurman, G., Kropshofer, H., Michel, B., Fattinger, C., & Delamarche, E. (2004) · Lab on a Chip · doi:10.1039/b408964b · Cited by 206
Proteomic patterns: their potential for disease diagnosis
Xiao, Z., Prieto, D. A., Conrads, T. P., Veenstra, T. D., & Issaq, H. J. (2004) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2004.10.010 · Cited by 190
Tang, L., Feng, H., Cheng, J., & Li, J. (2010) · Chemical Communications · doi:10.1039/c0cc01212b · Cited by 161
Self-citationLiu, X., & Ju, H. (2008) · Analytical Chemistry · doi:10.1021/ac8003715 · Cited by 159
Wang, X., Wang, C., Qu, K., Song, Y., Ren, J., Miyoshi, D., Sugimoto, N., & Qu, X. (2010) · Advanced Functional Materials · doi:10.1002/adfm.201001118 · Cited by 131
Radioimmunoassay: Review of basic principles
Goldsmith, S. J. (1975) · Seminars in Nuclear Medicine · doi:10.1016/s0001-2998(75)80028-6 · Cited by 123
Wang, X., Wang, C., Qu, K., Song, Y., Ren, J., Miyoshi, D., Sugimoto, N., & Qu, X. (2010) · Advanced Functional Materials · doi:10.1002/adfm.201090103 · Cited by 121
Sardesai, N. P., Pan, S., & Rusling, J. F. (2009) · Chemical Communications · doi:10.1039/b909220j · Cited by 115
Matsuya, T., Tashiro, S., Hoshino, N., et al. (2003) · Analytical Chemistry · doi:10.1021/ac034346e · Cited by 112
FAN, F. F., Park, S., Zhu, Y., et al. (2008) · Journal of the American Chemical Society · doi:10.1021/ja8086246 · Cited by 111
Wu, X., Hu, Y., Jin, J., Zhou, N., Wu, P., Zhang, H., & Cai, C. (2010) · Analytical Chemistry · doi:10.1021/ac100621r · Cited by 107
Saito, K., Kobayashi, D., Sasaki, M., Araake, H., Kida, T., Yagihashi, A., Yajima, T., Kameshima, H., & Watanabe, N. (1999) · Clinical Chemistry · doi:10.1093/clinchem/45.5.665 · Cited by 103
Electrochemiluminescence Biobarcode Method Based on Cysteamine−Gold Nanoparticle Conjugates
Duan, R., Zhou, X., & Xing, D. (2010) · Analytical Chemistry · doi:10.1021/ac100018z · Cited by 98
Saito, K., Kobayashi, D., Sasaki, M., Araake, H., Kida, T., Yagihashi, A., Yajima, T., Kameshima, H., & Watanabe, N. (1999) · PubMed · Cited by 96
Pinijsuwan, S., Rijiravanich, P., Somasundrum, M., & Surareungchai, W. (2008) · Analytical Chemistry · doi:10.1021/ac800566d · Cited by 90
Ala‐Kleme, T., Mäkinen, P., Ylinen-Hinkka, T., Väre, L., Kulmala, S., Ihalainen, P., & Peltonen, J. P. K. (2005) · Analytical Chemistry · doi:10.1021/ac051157i · Cited by 45
Chen, X.-M., Wu, G., Chen, J., Jiang, Y., Chen, G., Oyama, M., Chen, X., & Wang, X. (2010) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2010.07.083 · Cited by 28
Cited by
New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide
Feng, L., Wu, L., & Qu, X. (2013) · Advanced Materials · doi:10.1002/adma.201203229 · Cited by 632
Nanocarbons for Biology and Medicine: Sensing, Imaging, and Drug Delivery
Panwar, N., Soehartono, A. M., Chan, K. K., Zeng, S., Xu, G., Qu, J., Coquet, P., Yong, K.-T., & Chen, X. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00099 · Cited by 555
Metadata
- Authors
- Xu, S., Liu, Y., Wang, T., & Li, J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-04-22
- Volume
- 83
- Issue
- 10
- Pages
- 3817-3823
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2329183394
- Cited by
- 367
- References
- 43
- FWCI
- 15.28
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
