Cited by 189Microfluidic and Bio-sensing TechnologiesMicrofluidic and Capillary Electrophoresis Applications3D Printing in Biomedical Research

Microfluidic Chip with Integrated Electrical Cell-Impedance Sensing for Monitoring Single Cancer Cell Migration in Three-Dimensional Matrixes

Nguyen, Tien Anh, Yin, Tsung-I, Reyes, Diego, Urban, Gerald A.·2013·Analytical Chemistry

Abstract

Cell migration has been recognized as one hallmark of malignant tumor progression. By integrating the method of electrical cell-substrate impedance sensing (ECIS) with the Boyden chamber design, the state-of-the-art techniques provide kinetic information about cell migration and invasion processes in three-dimensional (3D) extracellular matrixes. However, the information related to the initial stage of cell migration with single-cell resolution, which plays a unique role in the metastasis-invasion cascade of cancer, is not yet available. In this paper, we present a microfluidic device integrated with ECIS for investigating single cancer cell migration in 3D matrixes. Using microfluidics techniques without the requirement of physical connections to off-chip pneumatics, the proposed sensor chip can efficiently capture single cells on microelectrode arrays for sequential on-chip 2D or 3D cell culture and impedance measurement. An on-chip single-cell migration assay was successfully demonstrated within several minutes. Migration of single metastatic MDA-MB-231 cells in their initial stage can be monitored in real time; it shows a rapid change in impedance magnitude of approximately 10 Ω/s, whereas no prominent impedance change is observed for less-metastasis MCF-7 cells. The proposed sensor chip, allowing for a rapid and selective detection of the migratory properties of cancer cells at the single-cell level, could be applied as a new tool for cancer research.

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Crossref · 10.1021/ac402761s

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Authors
Nguyen, T. A., Yin, T.-I., Reyes, D., & Urban, G. A.
Affiliations
University of Freiburg (DE)
Corresponding author
Nguyen, Tien Anh
Venue
Analytical Chemistry
Published
2013-11-08
Volume
85
Issue
22
Pages
11068-11076
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W2331001184
Cited by
189
References
36
Citations by year
2026: 9 · 2025: 8 · 2024: 10 · 2023: 18 · 2022: 10 · 2021: 16 · 2020: 14 · 2019: 23 · 2018: 17 · 2017: 16 · 2016: 18 · 2015: 23 · 2014: 7
FWCI
8.82
Type
journal article
Language
en
Open access
closed
Locations
  • Analytical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
electrical cell-substrate impedance sensing, single-cell migration, tumor cell migration, microfluidics, three-dimensional extracellular matrix, metastasis, MDA-MB-231 cells, MCF-7 cells, microelectrode arrays, on-chip cell culture, impedance measurement
MeSH terms
Breast Neoplasms, Cell Movement, Electrodes, Female, Humans, Monitoring, Physiologic, Tumor Cells, Cultured, Biosensing Techniques, Electric Impedance, Cell Culture Techniques, Microfluidics, Microchip Analytical Procedures

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