The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
High-Speed DNA Genotyping Using Microfabricated Capillary Array Electrophoresis Chips
Abstract
Capillary array electrophoresis (CAE) chips have been designed and fabricated with the capacity to rapidly (< 160 s) analyze 12 different samples in parallel. Detection of all lanes with 0.3 s temporal resolution was achieved using a laser-excited confocal-fluorescence scanner. The operation and capabilities of these CAE microdevices were first determined by performing electrophoretic separations of pBR322 MspI DNA samples. Genotyping of HLA-H, a candidate gene for the diagnosis of hereditary hemochromatosis, was then performed to demonstrate the rapid analysis of biologically relevant samples. Two-color multiplex fluorescence detection of HLA-H genotypes was accomplished by prelabeling the standard pBR322 MspI DNA ladder with a red emitting bis-intercalation dye (butyl TOTIN) and on-column labeling of the HLA-H DNA with thiazole orange. This work establishes the feasibility of using CAE chips for high speed, high-throughput genotyping.
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Cites
A simple salting out procedure for extracting DNA from human nucleated cells
Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.3.1215 · Cited by 20,330
A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis
Feder, J. N., Gnirke, A., Thomas, W., Tsuchihashi, Z., Ruddy, D. A., Basava, A., Dormishian, F., Domingo, R., Ellis, M. C., Fullan, A., et al. (1996) · Nature Genetics · doi:10.1038/ng0896-399 · Cited by 3,664
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
Harrison, D. J., Fluri, K., Seiler, K., Fan, Z., Effenhauser, C. S., & Manz, A. (1993) · Science · doi:10.1126/science.261.5123.895 · Cited by 1,800
Capillary electrophoresis and sample injection systems integrated on a planar glass chip
Harrison, D. J., Manz, A., Fan, Z., Luedi, H., & Widmer, H. M. (1992) · Analytical Chemistry · doi:10.1021/ac00041a030 · Cited by 1,226
(1998) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826079808001954 · Cited by 1,072
Manz, A., Harrison, D. J., Verpoorte, E. M. J., Fettinger, J. C., Paulus, A., Lüdi, H., & Widmer, H. M. (1992) · Journal of Chromatography A · doi:10.1016/0021-9673(92)80293-4 · Cited by 1,042
Woolley, A. T., Hadley, D., Landre, P., deMello, A. J., Mathies, R. A., & Northrup, M. A. (1996) · Analytical Chemistry · doi:10.1021/ac960718q · Cited by 730
Self-citationWoolley, A. T., & Mathies, R. A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.24.11348 · Cited by 605
Self-citationUltra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
Woolley, A. T., & Mathies, R. A. (1995) · Analytical Chemistry · doi:10.1021/ac00116a010 · Cited by 564
Self-citationCapillary gel electrophoresis for rapid, high resolution DNA sequencing
Swerdlow, H., & Gesteland, R. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.6.1415 · Cited by 360
Cited by
Microfluidic Devices for Bioapplications
Yeo, L. Y., Chang, H., Chan, P. P. Y., & Friend, J. R. (2011) · Small · doi:10.1002/smll.201000946 · Cited by 622
Microfluidic chips for clinical and forensic analysis
Verpoorte, E. (2002) · ELECTROPHORESIS · doi:10.1002/1522-2683(200203)23:5<677::aid-elps677>3.0.co;2-8 · Cited by 526
Preservation of key biomolecules in the fossil record: current knowledge and future challenges
Bada, J. L., Wang, X. S., & Hamilton, H. (1999) · Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rstb.1999.0361 · Cited by 147
Metadata
- Authors
- Woolley, A. T., Sensabaugh, G. F., & Mathies, R. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-06-01
- Volume
- 69
- Issue
- 11
- Pages
- 2181-2186
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2058877820
- Cited by
- 310
- References
- 19
- FWCI
- 33.55
- Type
- journal article
- Language
- en
- Open access
- closed
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