The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
High-Speed DNA Genotyping Using Microfabricated Capillary Array Electrophoresis Chips
Abstract
Capillary array electrophoresis (CAE) chips have been designed and fabricated with the capacity to rapidly (< 160 s) analyze 12 different samples in parallel. Detection of all lanes with 0.3 s temporal resolution was achieved using a laser-excited confocal-fluorescence scanner. The operation and capabilities of these CAE microdevices were first determined by performing electrophoretic separations of pBR322 MspI DNA samples. Genotyping of HLA-H, a candidate gene for the diagnosis of hereditary hemochromatosis, was then performed to demonstrate the rapid analysis of biologically relevant samples. Two-color multiplex fluorescence detection of HLA-H genotypes was accomplished by prelabeling the standard pBR322 MspI DNA ladder with a red emitting bis-intercalation dye (butyl TOTIN) and on-column labeling of the HLA-H DNA with thiazole orange. This work establishes the feasibility of using CAE chips for high speed, high-throughput genotyping.
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Citations
Cites
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Cited by
Microfluidic Devices for Bioapplications
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Microfluidic chips for clinical and forensic analysis
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Preservation of key biomolecules in the fossil record: current knowledge and future challenges
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Metadata
- Authors
- Woolley, A. T., Sensabaugh, G. F., & Mathies, R. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-06-01
- Volume
- 69
- Issue
- 11
- Pages
- 2181-2186
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2058877820
- Cited by
- 310
- References
- 19
- FWCI
- 33.55
- Type
- journal article
- Language
- en
- Open access
- closed
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