Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson’s Disease Diagnostics
Open accessAbstract
The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).
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The new nanotheranostic platform displayed improved sensitivity, with a fivefold reduced necessary sample volume for analysis compared to an electrochemiluminescence kit.
“The new nanotheranostic platform displayed improved sensitivity, with a fivefold reduced necessary sample volume for analysis compared to an electrochemiluminescence kit [252].”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [252]
The “Label-Free Impedimetric Assays in Spiked Serum” section says the assay is more sensitive than commercial electrochemiluminescence kits and requires 100 μL rather than 500 μL of sample—a fivefold reduction. The Conclusions describe it as a diagnostic impedimetric assay, not a nanotheranostic platform; our copy does not report a therapeutic function. (LLM, conf=0.88)
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Self-citation
- Authors
- Fu, Y., Jiang, C., Tofaris, G. K., & Davis, J. J.
- Affiliations
- University of Oxford (GB)
- John Radcliffe Hospital (GB)
- Corresponding author
- Tofaris, George K.
- Davis, Jason J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-09-18
- Volume
- 92
- Issue
- 20
- Pages
- 13647-13651
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- PMC
- PMC7584333
- OpenAlex
- W3087073012
- Cited by
- 74
- References
- 44
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 15 · 2023: 21 · 2022: 12 · 2021: 10
- FWCI
- 3.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Analytical Chemistry (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (acceptedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/M006204/1)
- NIHR Oxford Biomedical Research Centre (NA)
- Selfridges Group Foundation (NA)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- Parkinson's disease, alpha-synuclein, syntenin-1, electrochemical impedance spectroscopy, reagent-free detection, serum biomarkers, zwitterionic polymers, disease diagnosis
- MeSH terms
- Humans, Magnetics, Parkinson Disease, Polymers, Biomarkers, Neural Cell Adhesion Molecule L1, alpha-Synuclein, Syntenins, Exosomes, Antibodies, Immobilized, Limit of Detection, Dielectric Spectroscopy
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