Cited by 74Electrochemical sensors and biosensorsExtracellular vesicles in diseaseAdvanced biosensing and bioanalysis techniques

Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson’s Disease Diagnostics

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Fu, Ying, Jiang, Cheng, Tofaris, George K., Davis, Jason J.·2020·Analytical Chemistry

Abstract

The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).

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Crossref · 10.1021/acs.analchem.0c03092

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  • The new nanotheranostic platform displayed improved sensitivity, with a fivefold reduced necessary sample volume for analysis compared to an electrochemiluminescence kit.

    “The new nanotheranostic platform displayed improved sensitivity, with a fivefold reduced necessary sample volume for analysis compared to an electrochemiluminescence kit [252].”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [252]

    The “Label-Free Impedimetric Assays in Spiked Serum” section says the assay is more sensitive than commercial electrochemiluminescence kits and requires 100 μL rather than 500 μL of sample—a fivefold reduction. The Conclusions describe it as a diagnostic impedimetric assay, not a nanotheranostic platform; our copy does not report a therapeutic function. (LLM, conf=0.88)

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Research works printed but not linked: 1

  1. Taylor D. D.; Zacharias W.; Gercel-Taylor C. In Serum/Plasma Proteomics: Methods and Protocols; Humana Press: Totowa, NJ, 2011; pp 235– 246.

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Authors
Fu, Y., Jiang, C., Tofaris, G. K., & Davis, J. J.
Affiliations
  • University of Oxford (GB)
  • John Radcliffe Hospital (GB)
Corresponding author
  • Tofaris, George K.
  • Davis, Jason J.
Venue
Analytical Chemistry
Published
2020-09-18
Volume
92
Issue
20
Pages
13647-13651
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
OpenAlex
W3087073012
Cited by
74
References
44
Citations by year
2026: 5 · 2025: 11 · 2024: 15 · 2023: 21 · 2022: 12 · 2021: 10
FWCI
3.69
Type
journal article
Language
en
Open access
Locations
  • Analytical Chemistry (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (acceptedVersion)
Funders
  • Engineering and Physical Sciences Research Council (EP/M006204/1)
  • NIHR Oxford Biomedical Research Centre (NA)
  • Selfridges Group Foundation (NA)
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords
Parkinson's disease, alpha-synuclein, syntenin-1, electrochemical impedance spectroscopy, reagent-free detection, serum biomarkers, zwitterionic polymers, disease diagnosis
MeSH terms
Humans, Magnetics, Parkinson Disease, Polymers, Biomarkers, Neural Cell Adhesion Molecule L1, alpha-Synuclein, Syntenins, Exosomes, Antibodies, Immobilized, Limit of Detection, Dielectric Spectroscopy

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