The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
CRISPR-Cas12a-Derived Photoelectrochemical Biosensor for Point-Of-Care Diagnosis of Nucleic Acid
Abstract
This work presented a point-of-care (POC) photoelectrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on a portable electrochemical detection system by using CRISPR-Cas12a trans-cleaving the G-quadruplex for the biorecognition/amplification and a hollow In 2 O 3 –In 2 S 3 -modified screen-printed electrode (In 2 O 3 –In 2 S 3 /SPE) as the photoactive material. G-quadruplexes were capable of biocatalytic precipitation (H 2 O 2 -mediated 4-chloro-1-naphthol oxidation) on the In 2 O 3 –In 2 S 3 /SPE surface, resulting in a weakened photocurrent, but suffered from trans-cleavage when the CRISPR-Cas12a system specifically recognized the analyte. The photocurrent results could be directly observed with the card-sized electrochemical device via a smartphone, which displayed a high-value photocurrent for these positive samples, while a low-value photocurrent for the target-free samples. Such a system exhibited satisfying photocurrent responses toward HPV-16 within a wide working range from 5.0 to 5000 pM and allowed for detection of HPV-16 at a concentration as low as 1.2 pM. The proposed assay provided a smartphone signal readout to enable the rapid screening PEC determination of HPV-16 concentration without sophisticated instruments, thus meeting the requirements of remote areas and resource-limited settings. We envision that combining an efficient biometric PEC sensing platform with a wireless card-sized electrochemical device will enable high-throughput POC diagnostic analysis.
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Cites
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aar6245 · Cited by 4,507
Shu, J., & Tang, D. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b04199 · Cited by 976
Self-citationXiong, Y., Zhang, J., Yang, Z., Mou, Q., Ma, Y., Xiong, Y., & Lu, Y. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b09211 · Cited by 689
Smartphone-based biosensors: A critical review and perspectives
Roda, A., Michelini, E., Zangheri, M., Di Fusco, M., Calabria, D., & Simoni, P. (2016) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2015.10.019 · Cited by 520
CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor
Zeng, R., Wang, W., Chen, M., Wan, Q., Wang, C., Knopp, D., & Tang, D. (2021) · Nano Energy · doi:10.1016/j.nanoen.2020.105711 · Cited by 394
Self-citationZeng, R., Luo, Z., Zhang, L., & Tang, D. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b03889 · Cited by 355
Self-citationPaper Electrode-Based Flexible Pressure Sensor for Point-of-Care Immunoassay with Digital Multimeter
Yu, Z., Tang, Y., Cai, G., Ren, R., & Tang, D. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.8b04635 · Cited by 341
Self-citationSmartphone-based analytical biosensors
Huang, X., Xu, D., Chen, J., Liu, J., Li, Y., Song, J., Ma, X., & Guo, J. (2018) · The Analyst · doi:10.1039/c8an01269e · Cited by 305
Self-citationBeduk, T., Beduk, D., de Oliveira Filho, J. I., Zihnioglu, F., Cicek, C., Sertoz, R., Arda, B., Goksel, T., Turhan, K., Salama, K. N., et al. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c01444 · Cited by 171
Uric Acid Monitoring with a Smartphone as the Electrochemical Analyzer
Guo, J. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b04345 · Cited by 166
Cited by
CRISPR-Driven Biosensors: A New Frontier in Rapid and Accurate Disease Detection
Razavi, Z., Soltani, M., Souri, M., & Pazoki-Toroudi, H. (2026) · Critical Reviews in Analytical Chemistry · doi:10.1080/10408347.2024.2400267 · Cited by 45
Metadata
- Authors
- Zeng, R., Gong, H., Li, Y., Li, Y., Lin, W., Tang, D., & Knopp, D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2022-05-12
- Volume
- 94
- Issue
- 20
- Pages
- 7442-7448
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W4280578891
- Cited by
- 404
- References
- 16
- FWCI
- 24.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zeng, R., Gong, H., Li, Y., Li, Y., Lin, W., Tang, D., & Knopp, D. (2022). CRISPR-Cas12a-Derived Photoelectrochemical Biosensor for Point-Of-Care Diagnosis of Nucleic Acid. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.2c01373
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