The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Nanoliter-Scale Oil-Air-Droplet Chip-Based Single Cell Proteomic Analysis
Abstract
Single cell proteomic analysis provides crucial information on cellular heterogeneity in biological systems. Herein, we describe a nanoliter-scale oil-air-droplet (OAD) chip for achieving multistep complex sample pretreatment and injection for single cell proteomic analysis in the shotgun mode. By using miniaturized stationary droplet microreaction and manipulation techniques, our system allows all sample pretreatment and injection procedures to be performed in a nanoliter-scale droplet with minimum sample loss and a high sample injection efficiency (>99%), thus substantially increasing the analytical sensitivity for single cell samples. We applied the present system in the proteomic analysis of 100 ± 10, 50 ± 5, 10, and 1 HeLa cell(s), and protein IDs of 1360, 612, 192, and 51 were identified, respectively. The OAD chip-based system was further applied in single mouse oocyte analysis, with 355 protein IDs identified at the single oocyte level, which demonstrated its special advantages of high enrichment of sequence coverage, hydrophobic proteins, and enzymatic digestion efficiency over the traditional in-tube system.
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Cites
Mass spectrometry-based proteomics
Aebersold, R., & Mann, M. (2003) · Nature · doi:10.1038/nature01511 · Cited by 6,955
Bendall, S. C., Simonds, E. F., Qiu, P., Amir, E.-A. D., Krutzik, P. O., Finck, R., Bruggner, R. V., Melamed, R., Trejo, A., Ornatsky, O. I., et al. (2011) · Science · doi:10.1126/science.1198704 · Cited by 2,279
Kulak, N. A., Pichler, G., Paron, I., Nagaraj, N., & Mann, M. (2014) · Nature Methods · doi:10.1038/nmeth.2834 · Cited by 1,719
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Ultrasensitive and Fast Bottom‐up Analysis of Femtogram Amounts of Complex Proteome Digests
Sun, L., Zhu, G., Zhao, Y., Yan, X., Mou, S., & Dovichi, N. J. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201308139 · Cited by 212
Cited by
Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation
Brunner, A., Thielert, M., Vasilopoulou, C., Ammar, C., Coscia, F., Mund, A., Hoerning, O. B., Bache, N., Apalategui, A., Lubeck, M., et al. (2022) · Molecular Systems Biology · doi:10.15252/msb.202110798 · Cited by 544
Unbiased spatial proteomics with single-cell resolution in tissues
Mund, A., Brunner, A.-D., & Mann, M. (2022) · Molecular Cell · doi:10.1016/j.molcel.2022.05.022 · Cited by 259
Single-cell protein analysis by mass spectrometry
Slavov, N. (2021) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2020.04.018 · Cited by 164
Single‐cell proteomics differentiates Arabidopsis root cell types
Montes, C., Zhang, J., Nolan, T. M., & Walley, J. W. (2024) · New Phytologist · doi:10.1111/nph.19923 · Cited by 48
Size, Morphology and Crystallinity Control Strategy of Ultrafine HMX by Microfluidic Platform
Jiang, H., Wang, X., Yu, J., Zhou, W., Zhao, S., Xu, S., & Zhao, F. (2023) · Nanomaterials · doi:10.3390/nano13030464 · Cited by 24
Self-citation
Metadata
- Authors
- Li, Z.-Y., Huang, M., Wang, X.-K., Zhu, Y., Li, J.-S., Wong, C. C. L., & Fang, Q.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-03-19
- Volume
- 90
- Issue
- 8
- Pages
- 5430-5438
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2790054076
- Cited by
- 229
- References
- 5
- FWCI
- 7.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Li, Z.-Y., Huang, M., Wang, X.-K., Zhu, Y., Li, J.-S., Wong, C. C. L., & Fang, Q. (2018). Nanoliter-Scale Oil-Air-Droplet Chip-Based Single Cell Proteomic Analysis. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.8b00661
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