Cited by 212Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMicrofluidic and Capillary Electrophoresis Applications

Ultrasensitive and Fast Bottom‐up Analysis of Femtogram Amounts of Complex Proteome Digests

Open access
Sun, Liangliang, Zhu, Guijie, Zhao, Yimeng, Yan, Xiaojing, Mou, Si, Dovichi, Norman J.·2013·Angewandte Chemie International Edition

Abstract

Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.

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Crossref · 10.1002/anie.201308139

Cites

22 of 22 references verified0 citation errors · 0 not checkable

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  • CZE has proven valuable for protein identification via bottom-up proteomics, where ultrasensitive and fast analysis can be obtained.

    “CZE has also proven to be a valuable tool for protein identification via bottom-up proteomics where ultrasensitive and fast analysis can be obtained72–74.”

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Authors
Sun, L., Zhu, G., Zhao, Y., Yan, X., Mou, S., & Dovichi, N. J.
Affiliations
University of Notre Dame (US)
Venue
Angewandte Chemie International Edition
Publisher
Wiley
Published
2013-10-31
Volume
52
Issue
51
Pages
13661-13664
ISSN
  • 1433-7851 (ISSN-L)
  • 1521-3773
OpenAlex
W2136528511
Cited by
212
References
22
Citations by year
2026: 10 · 2025: 13 · 2024: 12 · 2023: 4 · 2022: 16 · 2021: 17 · 2020: 20 · 2019: 20 · 2018: 30 · 2017: 21 · 2016: 20 · 2015: 17 · 2014: 11 · 2013: 1
FWCI
7.34
Type
journal article
Language
en
Open access
Locations
  • Angewandte Chemie International Edition (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (R01GM096767)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
shotgun proteomics, capillary zone electrophoresis-mass spectrometry, Escherichia coli proteome, tandem mass spectrometry, protein identification
MeSH terms
Electrophoresis, Proteome, Spectrometry, Mass, Electrospray Ionization, Proteomics

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