Ultrasensitive and Fast Bottom‐up Analysis of Femtogram Amounts of Complex Proteome Digests
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Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.
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CZE has proven valuable for protein identification via bottom-up proteomics, where ultrasensitive and fast analysis can be obtained.
“CZE has also proven to be a valuable tool for protein identification via bottom-up proteomics where ultrasensitive and fast analysis can be obtained72–74.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [72]
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Self-citation
- Authors
- Sun, L., Zhu, G., Zhao, Y., Yan, X., Mou, S., & Dovichi, N. J.
- Affiliations
- University of Notre Dame (US)
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2013-10-31
- Volume
- 52
- Issue
- 51
- Pages
- 13661-13664
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- PMC
- PMC3904452
- OpenAlex
- W2136528511
- Cited by
- 212
- References
- 22
- Citations by year
- 2026: 10 · 2025: 13 · 2024: 12 · 2023: 4 · 2022: 16 · 2021: 17 · 2020: 20 · 2019: 20 · 2018: 30 · 2017: 21 · 2016: 20 · 2015: 17 · 2014: 11 · 2013: 1
- FWCI
- 7.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Angewandte Chemie International Edition (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM096767)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- shotgun proteomics, capillary zone electrophoresis-mass spectrometry, Escherichia coli proteome, tandem mass spectrometry, protein identification
- MeSH terms
- Electrophoresis, Proteome, Spectrometry, Mass, Electrospray Ionization, Proteomics
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