Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry
Open accessAbstract
Development of chemical chaperones to solubilize membrane protein complexes in aqueous solutions has allowed for gas-phase analysis of their native-like assemblies, including rapid evaluation of stability and interacting partners. Characterization of protein primary sequence, however, has thus far been limited. Ultraviolet photodissociation (UVPD) generates a multitude of sequence ions for the E. coli ammonia channel (AmtB), provides improved localization of a possible post-translational modification of aquaporin Z (AqpZ), and surpasses previous reports of sequence coverage for mechanosensitive channel of large conductance (MscL). Variations in UVPD sequence ion abundance have been shown to correspond to structural changes induced upon some perturbation. Preliminary results are reported here for elucidating increased rigidity or flexibility of MscL when bound to various phospholipids.
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The sequence coverage for aquaporin Z monomers was increased by 21% using UVPD compared to direct native TD UVPD of the tetramer.
“The sequence coverage for monomers was increased by 21% in this way compared to direct native TD UVPD of the tetramer.”
From Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry (2020) · cites this paper as [109]
Brodbelt and co-workers used UVPD for effective sequencing of aquaporin Z monomers ejected from the native tetramer.
“Brodbelt and co-workers used UVPD for effective sequencing of aquaporin Z monomers ejected from the native tetramer.109”
From Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry (2020) · cites this paper as [109]
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- Authors
- Sipe, S. N., Patrick, J. W., Laganowsky, A., & Brodbelt, J. S.
- Affiliations
- The University of Texas at Austin (US)
- Texas A&M University (US)
- Corresponding author
- Brodbelt, Jennifer S.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-11-25
- Volume
- 92
- Issue
- 1
- Pages
- 899-907
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- PMC
- PMC6949371
- OpenAlex
- W2990475748
- Cited by
- 39
- References
- 51
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 6 · 2020: 3 · 2018: 2
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Welch Foundation (F-1155)
- National Institute of General Medical Sciences (DP2GM123486, RO1GM121714)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- membrane protein complexes, chemical chaperones, AmtB, aquaporin Z, protein sequence coverage, post-translational modification, gas-phase ion fragmentation, phospholipid binding, Escherichia coli
- MeSH terms
- Amino Acid Sequence, Escherichia coli, Ion Channels, Models, Molecular, Photolysis, Protein Processing, Post-Translational, Mass Spectrometry, Ultraviolet Rays, Aquaporins, Cation Transport Proteins, Escherichia coli Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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