CoChat
Sign Up
Cited by 279Electrochemical sensors and biosensorsDiabetes Management and ResearchEnzyme Catalysis and Immobilization

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Microneedle-Based Detection of Ketone Bodies along with Glucose and Lactate: Toward Real-Time Continuous Interstitial Fluid Monitoring of Diabetic Ketosis and Ketoacidosis

Teymourian, Hazhir, Moonla, Chochanon, Tehrani, Farshad, Vargas, Eva, Aghavali, Reza, Barfidokht, Abbas, Tangkuaram, Tanin, Mercier, Patrick P., Dassau, Eyal, Wang, Joseph·2020·Analytical Chemistry
DOI 10.1021/acs.analchem.9b05109
Open this paper in CoChat — free account

Abstract

Diabetic ketoacidosis (DKA), a severe complication of diabetes mellitus with potentially fatal consequences, is characterized by hyperglycemia and metabolic acidosis due to the accumulation of ketone bodies, which requires people with diabetes to monitor both glucose and ketone bodies. However, despite major advances in diabetes management mainly since the emergence of new-generation continuous glucose monitoring (CGM) devices capable of in vivo monitoring of glucose directly in the interstitial fluid (ISF), the continuous monitoring of ketone bodies is yet to be addressed. Here, we present the first use of a real-time continuous ketone bodies monitoring (CKM) microneedle platform. The system is based on the electrochemical monitoring of β-hydroxybutyrate (HB) as the dominant biomarker of ketone formation. Such real-time HB detection has been realized using the β-hydroxybutyrate dehydrogenase (HBD) enzymatic reaction and by addressing the major challenges associated with the stable confinement of the enzyme/cofactor couple (HBD/NAD + ) and with a stable and selective low-potential fouling-free anodic detection of NADH. The resulting CKM microneedle device displays an attractive analytical performance, with high sensitivity (with low detection limit, 50 μM), high selectivity in the presence of potential interferences, along with good stability during prolonged operation in artificial ISF. The potential applicability of this microneedle sensor toward minimally invasive monitoring of ketone bodies has been demonstrated in a phantom gel skin-mimicking model. The ability to detect HB along with glucose and lactate on a single microneedle array has been demonstrated. These findings pave the way for CKM and for the simultaneous microneedle-based monitoring of multiple diabetes-related biomarkers toward a tight glycemic control.

Overview

Integrity signals

Registry

Crossref · 10.1021/acs.analchem.9b05109

Cites

35 of 35 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 35 references

Cited by

Sorted by citations

Items 201–279 of 279

Related

See all

Metadata

Authors
Teymourian, H., Moonla, C., Tehrani, F., Vargas, E., Aghavali, R., Barfidokht, A., Tangkuaram, T., Mercier, P. P., Dassau, E., & Wang, J.
Affiliations
  • University of California San Diego (US)
  • Maejo University (TH)
  • Harvard University (US)
Corresponding author
  • Mercier, Patrick P.
  • Dassau, Eyal
  • Wang, Joseph
ORCID
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2019-12-24
Volume
92
Issue
2
Pages
2291-2300
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/acs.analchem.9b05109
OpenAlex
W2996840266
Cited by
278
References
35
Citations by year
2026: 30 · 2025: 50 · 2024: 53 · 2023: 53 · 2022: 44 · 2021: 27 · 2020: 21
FWCI
8.27
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Analytical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Leona M. and Harry B. Helmsley Charitable Trust (7505508-5108014 (2018PG-TI0061))
  • Center for Wearable Sensors, University of California San Diego
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
ketone bodies, beta-hydroxybutyrate, diabetic ketoacidosis, diabetic ketosis, microneedle sensors, interstitial fluid, electrochemical biosensor, beta-hydroxybutyrate dehydrogenase, NADH detection, glucose monitoring, lactate monitoring, diabetes mellitus
MeSH terms
Glucose, Humans, Ketone Bodies, Ketosis, Needles, Time Factors, Blood Glucose Self-Monitoring, Biosensing Techniques, Diabetic Ketoacidosis, Lactic Acid, Extracellular Fluid, Electrochemical Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review