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Single-Cell Chemical Proteomics (SCCP) Interrogates the Timing and Heterogeneity of Cancer Cell Commitment to Death

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Photoaffinity Labeling Chemistries Used to Map Biomolecular Interactions

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Combining experimental strategies for successful target deconvolution

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A Semiautomated Paramagnetic Bead-Based Platform for Isobaric Tag Sample Preparation

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Precipitate-Supported Thermal Proteome Profiling Coupled with Deep Learning for Comprehensive Screening of Drug Target Proteins

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Plant Extracts as a Source of Natural Products with Potential Antimalarial Effects: An Update from 2018 to 2022

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A Tale of Two Tails: Efficient Profiling of Protein Degraders by Specific Functional and Target Engagement Readouts

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A Phenotypic Screen Identifies Potent DPP9 Inhibitors Capable of Killing HIV-1 Infected Cells

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Dual molecule targeting HDAC6 leads to intratumoral CD4+ cytotoxic lymphocytes recruitment through MHC-II upregulation on lung cancer cells

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The Knowns and Unknowns in Protein–Metabolite Interactions

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A Comparison of Two Stability Proteomics Methods for Drug Target Identification in OnePot 2D Format

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Dienone Compounds: Targets and Pharmacological Responses

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Natural Product Alantolactone Targeting AKR1C1 Suppresses Cell Proliferation and Metastasis in Non-Small-Cell Lung Cancer

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Stability-based approaches in chemoproteomics

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Targeting pan-essential pathways in cancer with cytotoxic chemotherapy: challenges and opportunities

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A widespread family of viral sponge proteins reveals specific inhibition of nucleotide signals in anti-phage defense

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Nonionic Surfactants can Modify the Thermal Stability of Globular and Membrane Proteins Interfering with the Thermal Proteome Profiling Principles to Identify Protein Targets

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Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation

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A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand

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From Phenotypic Hit to Chemical Probe: Chemical Biology Approaches to Elucidate Small Molecule Action in Complex Biological Systems

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Mass Spectrometry-Based Chemical Proteomics for Drug Target Discoveries

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Target deconvolution with matrix-augmented pooling strategy reveals cell-specific drug-protein interactions

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Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assaysPreprint

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Microparticle-Assisted Precipitation Screening Method for Robust Drug Target Identification

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System-Wide Profiling by Proteome Integral Solubility Alteration Assay of Drug Residence Times for Target Characterization

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Aryl amino acetamides prevent Plasmodium falciparum ring development via targeting the lipid-transfer protein PfSTART1

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Chemoproteomic-enabled characterization of small GTPase Rab1a as a target of an N -arylbenzimidazole ligand's rescue of Parkinson's-associated cell toxicity

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Pharmacological induction of chromatin remodeling drives chemosensitization in triple-negative breast cancer

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Cellular thermal shift assay: an approach to identify and assess protein target engagement

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ProSAP: a GUI software tool for statistical analysis and assessment of thermal stability data

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Multifaceted Proteome Analysis at Solubility, Redox, and Expression Dimensions for Target Identification

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Thermal unfolding methods in drug discovery

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Distinct molecular targets of ProEGCG from EGCG and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

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High-throughput identification of calcium-regulated proteins across diverse proteomes

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New applications of advanced instrumental techniques for the characterization of food allergenic proteins

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Target-agnostic discovery of Rett Syndrome therapeutics by coupling computational network analysis and CRISPR-enabled in vivo disease modelingPreprint

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Lansoprazole interferes with fungal respiration and acts synergistically with amphotericin B against multidrug-resistant <i>Candida auris</i>

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Integrated Thermal Proteome Profiling and Affinity Ultrafiltration Mass Spectrometry (iTPAUMS): A Novel Paradigm for Elucidating the Mechanism of Action of Natural Products

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Proteome integral solubility alteration high-throughput proteomics assay identifies Collectin-12 as a non-apoptotic microglial caspase-3 substrate

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A Shift in Thinking: Cellular Thermal Shift Assay-Enabled Drug Discovery

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Natural product virtual-interact-phenotypic target characterization: A novel approach demonstrated with Salvia miltiorrhiza extract

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Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

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Decoding protein methylation function with thermal stability analysis

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Mechanistic Insights into a CDK9 Inhibitor Via Orthogonal Proteomics Methods

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Translating Senotherapeutic Interventions into the Clinic with Emerging Proteomic Technologies

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Experimental and data analysis advances in thermal proteome profiling

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COVID-19: Nanomedicine Uncovers Blood-Clot Mystery

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Solvent‐induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs

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Prediction of Molecular Initiating Events for Adverse Outcome Pathways Using High-Throughput Identification of Chemical Targets

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Metabolic modulation of mitochondrial mass during CD4+ T cell activation

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An In-Silico, In-Vitro and In-Vivo Combined Approach to Identify NMNATs as Potential Protein Targets of ProEGCG for Treatment of Endometriosis

Hung, S. W., Liang, B., Gao, Y., Zhang, R., Tan, Z., Zhang, T., Chung, J. P. W., Chan, T. H., & Wang, C. C. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.714790 · Cited by 12 · see citing works

Comparative Analysis of Protein Folding Stability-Based Profiling Methods for Characterization of Biological Phenotypes

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Integrated Protein Solubility Shift Assays for Comprehensive Drug Target Identification on a Proteome-Wide Scale

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Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays

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Poly-pharmacology of existing drugs: How to crack the code?

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Ion-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein–Molecule Interactions

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One-Pot Time-Induced Proteome Integral Solubility Alteration Assay for Automated and Sensitive Drug–Target Identification

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Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution

Zhang, X., Lytovchenko, O., Lundström, S. L., Zubarev, R. A., & Gaetani, M. (2022) · BIO-PROTOCOL · doi:10.21769/bioprotoc.4556 · Cited by 31 · see citing works

One-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identificationPreprint

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Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay

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