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Cited by 25Tissue Engineering and Regenerative MedicineCorneal Surgery and TreatmentsHydrogels: synthesis, properties, applications

Novel Corneal Endothelial Cell Carrier Couples a Biodegradable Polymer and a Mesenchymal Stem Cell-Derived Extracellular Matrix

Song, Eui Sun, Park, Joo-Hee, Ha, Sang Su, Cha, Pu-hyeon, Kang, Jung-Taek, Park, Choul Yong, Park, Kwideok·2022·ACS Applied Materials & Interfaces
DOI 10.1021/acsami.2c01709
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Abstract

Here, we report a transparent, biodegradable, and cell-adhesive carrier that is securely coupled with the extracellular matrix (ECM) for corneal endothelial cell (CEC) transplantation. To fabricate a CEC carrier, poly(lactide- co -caprolactone) (PLCL) solution was poured onto the decellularized ECM (UMDM) derived from in vitro cultured umbilical cord blood-MSCs. Once completely dried, ECM–PLCL was then peeled off from the substrate. It was 20 μm thick, transparent, rich in fibronectin and collagen type IV, and easy to handle. Surface characterizations exhibited that ECM–PLCL was very rough (54.0 ± 4.50 nm) and uniformly covered in high density by ECM and retained a positive surface charge (65.2 ± 57.8 mV), as assessed via atomic force microscopy. Human CECs (B4G12) on the ECM–PLCL showed good cell attachment, with a cell density similar to the normal cornea. They could also maintain a cell phenotype, with nicely formed cell–cell junctions as assessed via ZO-1 and N-cadherin at 14 days. This was in sharp contrast to the CEC behaviors on the FNC-coated PLCL (positive control). A function-related marker, Na + /K + -ATPase, was also identified via western blot and immunofluorescence. In addition, primary rabbit CECs showed a normal shape and they could express structural and functional proteins on the ECM–PLCL. A simulation test confirmed that CECs loaded on the ECM–PLCL were successfully engrafted into the decellularized porcine corneal tissue, with a high engraftment level and cell viability. Moreover, ECM–PLCL transplantation into the anterior chamber of the rabbit eye for 8 weeks proved the maintenance of normal cornea properties. Taken together, this study demonstrates that our ECM–PLCL can be a promising cornea endothelium graft with an excellent ECM microenvironment for CECs.

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Crossref · 10.1021/acsami.2c01709

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  • ECM-PLCL scaffold maintains normal corneal characteristics when transplanted into rabbit eye; CEC has good adhesion, cell density similar to normal cornea, and cell phenotype maintained.

    “The results showed that CEC had a good adhesion effect on the scaffold, the cell density was similar to that of normal cornea, and the cell phenotype could be maintained. The animal experiment results showed that the ECM-PLCL scaffold could maintain normal corneal characteristics when transplanted into the rabbit eye [216].”

    From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [216]

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Authors
Song, E. S., Park, J.-H., Ha, S. S., Cha, P.-H., Kang, J.-T., Park, C. Y., & Park, K.
Affiliations
  • Korea Institute of Science and Technology (KR)
  • Dongguk University Ilsan Hospital (KR)
  • Biotechnology Research Institute, Mgenplus Co., Ltd., Seoul 06688, Republic of Korea
Corresponding author
  • Park, Choul Yong
  • Park, Kwideok
ORCID
Venue
ACS Applied Materials & Interfaces
Publisher
American Chemical Society (ACS)
Published
2022-03-03
Volume
14
Issue
10
Pages
12116-12129
ISSN
  • 1944-8244 (ISSN-L)
  • 1944-8252
DOI
10.1021/acsami.2c01709
OpenAlex
W4214932180
Cited by
25
References
42
Citations by year
2026: 3 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 3
FWCI
5.25
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • ACS Applied Materials & Interfaces (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Research Foundation of Korea (2020R1A2C2007972)
  • Ministry of Trade, Industry and Energy (20003915)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Corneal Surgery and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
corneal endothelial cell transplantation, decellularized extracellular matrix, umbilical cord blood mesenchymal stem cells, biodegradable polymer carriers, cell engraftment, Na+/K+-ATPase, ZO-1, N-cadherin, cell-cell contact, rabbit cornea
MeSH terms
Animals, Cells, Cultured, Extracellular Matrix, Polymers, Rabbits, Swine, Tissue Engineering, Endothelial Cells, Mesenchymal Stem Cells

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