Dumbbell Aptamer Sensor Based on Dual Biomarkers for Early Detection of Alzheimer’s Disease
Abstract
Finding a timely, sensitive, and noninvasive detection method has become an urgent need for asymptomatic early diagnosis of Alzheimer's disease (AD). MicroRNA-193b (miR-193b) and Aβ42 oligomers (AβO42) in neurogenic exosomes were confirmed to reflect pathological changes in the AD early stage. The combination of two biomarkers is promising for the earlier detection of AD. In this study, a detection system based on the principle of the entropy-driven strand displacement reaction (ESDR) was developed, including a dumbbell detection probe (H), an indicator probe (R), and graphene oxide (GO). In the detection system, the two hairpins of H were opened by the interaction of miR-193b (T1) and AβO42 (T2) with the aptamer. Then R hybridized with H and began to displace T, initiating the next round of ESDR to achieve sensitive detection of T. GO specifically adsorbed free R and quenched the fluorescence, further reducing the intensity of the background signal. Both of these points provided the system with a more sensitive analytical performance. The detection limit of miR-193b was 77 pM and the detection limit of AβO42 was 53 pM. This sensor detected the change of "one increase (AβO42) and one decrease (miR-193b)" in the exosome sample. Additionally, results showed that this detection system could distinguish the model of early AD from the non-AD control, which was sufficient for earlier and more sensitive detection of AD. This strategy has strong specificity, high sensitivity, and easy operation, which provides broad prospects for the early diagnosis of AD.
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Zhou and colleagues developed a highly sensitive and non-invasive fluorescent biosensor for simultaneously detecting neurogenic exosomes-derived miRNA-193b and Aβ42 oligomers (AβO42) associated with early Alzheimer's disease.
“Recently, Zhou and colleagues have advanced the field of diagnostics with the development of a highly sensitive and non-invasive fluorescent biosensor for simultaneously detecting two critical biomarkers associated with the early stages of AD [236], as illustrated in Fig. 3b.”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [236]
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- Authors
- Zhou, J., Sun, Y., Zhang, J., Luo, F., Ma, H., Guan, M., Feng, J., & Dong, X.
- Affiliations
- Zhengzhou University (CN)
- Corresponding author
- Zhou, Jie
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2023-03-23
- Volume
- 15
- Issue
- 13
- Pages
- 16394-16407
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W4360600710
- Cited by
- 26
- References
- 41
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 6 · 2023: 2
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (U1904135)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Keywords
- Alzheimer's disease, early diagnosis, aptasensor, amyloid-beta oligomers, exosomes, graphene oxide, fluorescence quenching, nucleic acid biosensors
- MeSH terms
- Alzheimer Disease, Graphite, Humans, Biosensing Techniques, Biomarkers, MicroRNAs, Early Diagnosis, Aptamers, Nucleotide
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