Cited by 89Luminescence and Fluorescent MaterialsSupramolecular Chemistry and ComplexesAdvanced biosensing and bioanalysis techniques

Multifunctional Supramolecular Assemblies with Aggregation-Induced Emission (AIE) for Cell Line Identification, Cell Contamination Evaluation, and Cancer Cell Discrimination

Bai, Haotian, Liu, Zhiyang, Zhang, Tianfu, Du, Jian, Zhou, Chengcheng, He, Wei, Chau, Joe H. C., Kwok, Ryan T. K. +2 more·2020·ACS Nano

Abstract

It is undoubted the important role of cells in biology and medicine, but worldwide misidentified and cross-contaminated cell lines have caused much trouble in related fields. Herein, three kinds of supramolecular AIE (aggregation-induced emission) nanoassemblies were constructed by the host–guest interaction between tetraphenylethene (TPE) derivatives and cucurbit[8]uril (CB[8]). Based on the recognized mechanism of AIE, the TPE derivatives could achieve stronger fluorescence emission and higher fluorescence quantum yield after assembling with CB[8]. Moreover, the constructed supramolecular AIE complexes obtained well-confirmed nanostructures and exhibited different sizes and shapes. Correspondingly, they generated characteristic biological properties and fluorescence enhancement of cells. Inspired by the concept of Big Data Analysis, these fluorescence signals were further transformed into a unique fingerprint of cells via linear discriminant analysis. Immediately, we realized the veracious identification between a normal cell line, two cancer cell lines, and two metastasized cancer cell lines in a qualitative analysis. More importantly, it was well used to monitor the evaluation of cross-contaminated cells and the discrimination of cancer cells. As a proper bioapplication of ideal supramolecular nanomaterials, this system was easy to learn and apply, and the whole procedure was kept to 20 min, without cell disruption, centrifugation, or washing steps.

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Crossref · 10.1021/acsnano.0c03404

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Authors
Bai, H., Liu, Z., Zhang, T., Du, J., Zhou, C., He, W., Chau, J. H. C., Kwok, R. T. K., Lam, J. W. Y., & Tang, B. Z.
Affiliations
Hong Kong University of Science and Technology (HK)
Corresponding author
Tang, Ben Zhong
Venue
ACS Nano
Published
2020-06-02
Volume
14
Issue
6
Pages
7552-7563
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
OpenAlex
W3029002541
Cited by
89
References
51
Citations by year
2026: 5 · 2025: 10 · 2024: 17 · 2023: 12 · 2022: 18 · 2021: 19 · 2020: 8
FWCI
5.55
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Nano (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Funders
  • Research Grants Council, University Grants Committee (16305618, 16308016, A-HKUST605/16, C6009-17G)
  • Ministry of Science and Technology of the People's Republic of China (2018YFE0190200)
  • Innovation and Technology Commission (ITC-CNERC14SC01)
  • National Natural Science Foundation of China (21788102)
  • Shenzhen City (JCYJ20170818113530705, JCYJ20170818113602462, JCYJ20180507183832744)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
  • Physical Sciences › Chemistry › Organic Chemistry › Supramolecular Chemistry and Complexes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords
aggregation-induced emission, supramolecular nanoassemblies, tetraphenylethene derivatives, cucurbit[8]uril, host-guest interactions, cell line identification, cell line cross-contamination, cancer cell discrimination, fluorescence fingerprinting, linear discriminant analysis, fluorescence enhancement
MeSH terms
Cell Line Authentication, Neoplasms

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