Phototheranostics: Active Targeting of Orthotopic Glioma Using Biomimetic Proteolipid Nanoparticles
Abstract
Advances in phototheranostics revolutionized glioma intraoperative fluorescence imaging and phototherapy. However, the lack of desired active targeting agents for crossing the blood–brain barrier (BBB) significantly compromises the theranostic efficacy. In this study, biomimetic proteolipid nanoparticles (NPs) with U.S. Food and Drug Administration (FDA)-approved indocyanine green (ICG) were constructed to allow fluorescence imaging, tumor margin detection, and phototherapy of orthotopic glioma in mice. By embedding glioma cell membrane proteins into NPs, the obtained biomimetic ICG-loaded liposome (BLIPO-ICG) NPs could cross BBB and actively reach glioma at the early stage thanks to their specific binding to glioma cells due to their excellent homotypic targeting and immune escaping characteristics. High accumulation in the brain tumor with a signal to background ratio of 8.4 was obtained at 12 h post-injection. At this time point, the glioma and its margin were clearly visualized by near-infrared fluorescence imaging. Under the imaging guidance, the glioma tissue could be completely removed as a proof of concept. In addition, after NIR laser irradiation (1 W/cm 2, 5 min), the photothermal effect exerted by BLIPO-ICG NPs efficiently suppressed glioma cell proliferation with a 94.2% tumor growth inhibition. No photothermal damages of normal brain tissue and treatment-induced side effects were observed. These results suggest that the biomimetic proteolipid NP is a promising phototheranostic nanoplatform for brain-tumor-specific imaging and therapy.
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Membrane-camouflaged nanoparticles were proved to be soft and their active proteins were easy to be inserted.
“these membrane-camouflaged nanoparticles were proved to be “soft” and the active proteins were easy to be inserted.”
From The blood–brain barrier: Structure, regulation and drug delivery (2023) · cites this paper as [367]
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- Authors
- Jia, Y., Wang, X., Hu, D., Wang, P., Liu, Q., Zhang, X., Jiang, J., Liu, X., Sheng, Z., Liu, B., & Zheng, H.
- Affiliations
- Chinese Academy of Sciences (CN)
- Shaanxi Normal University (CN)
- University of Macau (MO)
- National University of Singapore (SG)
- Corresponding author
- Sheng, Zonghai
- Liu, Bin
- Zheng, Hairong
- ORCID
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-12-21
- Volume
- 13
- Issue
- 1
- Pages
- 386-398
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2905610837
- Cited by
- 211
- References
- 61
- Citations by year
- 2026: 8 · 2025: 18 · 2024: 27 · 2023: 39 · 2022: 43 · 2021: 29 · 2020: 34 · 2019: 13
- FWCI
- 7.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education of the People's Republic of China (2015CB755500, 2016TS059, GK201802002)
- National Research Foundation Singapore (R279-000-483-281)
- National Natural Science Foundation of China (81472846, 81527901, 81327801, 81827807)
- Shaanxi Normal University (16QNGG012)
- Shenzhen Key Laboratory of Ultrasound Imaging and Therapy
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Keywords
- phototheranostics, glioma, biomimetic nanoparticles, indocyanine green, blood-brain barrier penetration, tumor margin detection, near-infrared fluorescence imaging, photothermal therapy, active targeting, orthotopic glioma
- MeSH terms
- Theranostic Nanomedicine, Animals, Blood-Brain Barrier, Brain Neoplasms, Female, Glioma, Humans, Indocyanine Green, Mice, Inbred BALB C, Mice, Nude, Proteolipids, Surgery, Computer-Assisted, Biomimetic Materials, Mice, Nanoparticles, Hep G2 Cells, MCF-7 Cells
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