The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Insertion of nucleotides opposite apurinic apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides
Abstract
M13 DNA containing 20-30 apurinic/apyrimidinic (AP) sites per intact circular molecule was prepared by growing phage on an ung- dut- Escherichia coli mutant and treating the DNA with uracil N-glycosylase. AP sites obstruct in vitro DNA synthesis catalyzed by E. coli pol I. The position at which termination of synthesis occurs was determined for four enzymes. T4 DNA polymerase terminates one nucleotide before putative AP sites. DNA pol I, AMV reverse transcriptase, and DNA polymerase alpha terminate synthesis either before or at the site of an AP lesion depending on the particular sequence. We determined the identity of the nucleotide inserted opposite an AP site by synthesizing up to the lesion in a first-stage reaction using T4 DNA polymerase and then determining elongation in a second stage. Purines are inserted opposite AP sites more readily than pyrimidines, and dATP is more efficient than dGTP in promoting such elongation. The DNA-dependent conversion of dNTP to dNMP was determined in mixtures of all four dNTP's by using AP DNA. The production of dAMP from dATP occurs most readily. We conclude that there is an inherent specificity for the incorporation of adenine nucleotides opposite AP sites in this in vitro system. Insofar as the model system reflects in vivo mutational events, our data suggest that depurination should produce transversions and depyrimidination should produce transitions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
McDonell, M. W., Simon, M. N., & Studier, F. W. (1977) · Journal of Molecular Biology · doi:10.1016/s0022-2836(77)80102-2 · Cited by 1,585
Rate of depurination of native deoxyribonucleic acid
Lindahl, T., & Nyberg, B. (1972) · Biochemistry · doi:10.1021/bi00769a018 · Cited by 1,556
Lindahl, T. (1982) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.51.070182.000425 · Cited by 895
Messing, J. W., Gronenborn, B., Müller‐Hill, B., & Hopschneider, P. H. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.9.3642 · Cited by 705
Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid
Lindahl, T., & Andersson, A. (1972) · Biochemistry · doi:10.1021/bi00769a019 · Cited by 591
Infidelity of DNA synthesis associated with bypass of apurinic sites.
Schaaper, R. M., Kunkel, T. A., & Loeb, L. A. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.2.487 · Cited by 305
Base substitution mutations induced by metabolically activated aflatoxin B1.
Foster, P. L., Eisenstadt, E., & Miller, J. H. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.9.2695 · Cited by 274
Depurination causes mutations in SOS-induced cells.
Schaaper, R. M., & Loeb, L. A. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.3.1773 · Cited by 186
Eisenstadt, E., Warren, A. J., Porter, J., Atkins, D., & Miller, J. H. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.6.1945 · Cited by 181
Cited by
8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions.
Cheng, K. C., Cahill, D. S., Kasai, H., Nishimura, S., & Loeb, L. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48474-8 · Cited by 1,844
Reactions of oxyl radicals with DNA
Breen, A. P., & Murphy, J. A. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)00209-3 · Cited by 1,038
MUTAGENESIS BY APURINIC/APYRIMIDINIC SITES
Loeb, L. A., & Preston, B. D. (1986) · Annual Review of Genetics · doi:10.1146/annurev.ge.20.120186.001221 · Cited by 901
Calorie restriction and aging: review of the literature and implications for studies in humans
Heilbronn, L. K., & Ravussin, E. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.3.361 · Cited by 701
Aguilar, F., Hussain, S. P., & Cerutti, P. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.18.8586 · Cited by 489
Metadata
- Authors
- Sagher, D., & Strauss, B.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1983-09-13
- Volume
- 22
- Issue
- 19
- Pages
- 4518-4526
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2092864864
- Cited by
- 320
- References
- 25
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Insertion of nucleotides opposite apurinic apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides. https://app.cochat.ai/papers/10.1021/bi00288a026
- The paper
- Sagher, D., & Strauss, B. (1983). Insertion of nucleotides opposite apurinic apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: uniqueness of adenine nucleotides. Biochemistry. https://doi.org/10.1021/bi00288a026
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
