Cited by 4783D Printing in Biomedical ResearchCancer Cells and MetastasisMathematical Biology Tumor Growth

3D cell culture systems modeling tumor growth determinants in cancer target discovery

Thoma, Claudio R., Zimmermann, Miriam, Agarkova, Irina, Kelm, Jens M., Krek, Wilhelm·2014·Advanced Drug Delivery Reviews

Abstract

Phenotypic heterogeneity of cancer cells, cell biological context, heterotypic crosstalk and the microenvironment are key determinants of the multistep process of tumor development. They sign responsible, to a significant extent, for the limited response and resistance of cancer cells to molecular-targeted therapies. Better functional knowledge of the complex intra- and intercellular signaling circuits underlying communication between the different cell types populating a tumor tissue and of the systemic and local factors that shape the tumor microenvironment is therefore imperative. Sophisticated 3D multicellular tumor spheroid (MCTS) systems provide an emerging tool to model the phenotypic and cellular heterogeneity as well as microenvironmental aspects of in vivo tumor growth. In this review we discuss the cellular, chemical and physical factors contributing to zonation and cellular crosstalk within tumor masses. On this basis, we further describe 3D cell culture technologies for growth of MCTS as advanced tools for exploring molecular tumor growth determinants and facilitating drug discovery efforts. We conclude with a synopsis on technological aspects for on-line analysis and post-processing of 3D MCTS models.

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Crossref · 10.1016/j.addr.2014.03.001

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Authors
Thoma, C. R., Zimmermann, M., Agarkova, I., Kelm, J. M., & Krek, W.
Affiliations
ETH Zurich (CH)
Corresponding author
  • Thoma, Claudio R.
  • Krek, Wilhelm
Venue
Advanced Drug Delivery Reviews
Publisher
Elsevier BV
Published
2014-04
Volume
69-70
Pages
29-41
ISSN
0169-409X (ISSN-L)
OpenAlex
W1976767579
Cited by
478
References
155
Citations by year
2026: 9 · 2025: 24 · 2024: 32 · 2023: 31 · 2022: 29 · 2021: 48 · 2020: 60 · 2019: 51 · 2018: 56 · 2017: 46 · 2016: 48 · 2015: 35 · 2014: 9
FWCI
3.93
Type
journal article
Language
en
Open access
closed
Locations
  • Advanced Drug Delivery Reviews (publishedVersion)
  • PubMed (publishedVersion)
Funders
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Physical Sciences › Mathematics › Modeling and Simulation › Mathematical Biology Tumor Growth
Keywords
tumor spheroids, 3D cell culture, tumor microenvironment, tumor heterogeneity, cell-cell crosstalk, anticancer drug discovery, drug resistance, targeted therapy
MeSH terms
Animals, Antineoplastic Agents, Humans, Neoplasms, Spheroids, Cellular, Cell Culture Techniques, Drug Discovery, Tumor Microenvironment

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