The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Assembly and Stability of Nisin−Lipid II Pores
Abstract
The peptide antibiotic nisin was the first reported example of an antibiotic that kills bacteria via targeted pore formation. The specific target of nisin is Lipid II, an essential intermediate in the bacterial cell-wall synthesis. High-affinity binding of the antibiotic to Lipid II is followed by rapid permeabilization of the membrane. Here, we investigated the assembly and stability of nisin-Lipid II pore complexes by means of pyrene fluorescence and circular dichroism. We demonstrated that nisin uses all available Lipid II molecules in the membrane to form pore complexes. The pore complexes have a uniform structure and consist of 8 nisin and 4 Lipid II molecules. Moreover, the pores displayed a remarkable stability, because they were able to resist the solubilization of the membrane environment by mild detergents. Similar experiments with [N20P/M21P]nisin showed that the hinge region is essential for the assembly into stable pore complexes. The new insights were used to propose a refined model for nisin pore formation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,947
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Rouser, G., Fleischer, S., & Yamamoto, A. (1970) · Lipids · doi:10.1007/bf02531316 · Cited by 3,274
Hope, M. J., Bally, M. B., Webb, G., & Cullis, P. R. (1985) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(85)90521-8 · Cited by 2,076
Woody, R. W. (1995) · Methods in Enzymology · doi:10.1016/0076-6879(95)46006-3 · Cited by 1,034
Applications of the bacteriocin, nisin
Delves-Broughton, J., Blackburn, P., Evans, R. J., & Hugenholtz, J. (1996) · Antonie van Leeuwenhoek · doi:10.1007/bf00399424 · Cited by 972
Use of the Cell Wall Precursor Lipid II by a Pore-Forming Peptide Antibiotic
Breukink, E., Wiedemann, I., Kraaij, C. V., Kuipers, O. P., Sahl, H.-G., & de Kruijff, B. (1999) · Science · doi:10.1126/science.286.5448.2361 · Cited by 814
Self-citationWiedemann, I., Breukink, E., van Kraaij, C., Kuipers, O. P., Bierbaum, G., de Kruijff, B., & Sahl, H.-G. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006770200 · Cited by 790
Self-citationSahl, H.-G., & Bierbaum, G. (1998) · Annual Review of Microbiology · doi:10.1146/annurev.micro.52.1.41 · Cited by 476
Brötz, H., Josten, M., Wiedemann, I., Schneider, U., Götz, F., Bierbaum, G., & Sahl, H. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01065.x · Cited by 421
Cited by
Bacillus subtilis antibiotics: structures, syntheses and specific functions
Stein, T. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04587.x · Cited by 1,507
Biosynthesis and Mode of Action of Lantibiotics
Chatterjee, C., Paul, M., Xie, L., & van der Donk, W. A. (2005) · Chemical Reviews · doi:10.1021/cr030105v · Cited by 718
Lantibiotics: Peptides of Diverse Structure and Function
Willey, J. M., & van der Donk, W. A. (2007) · Annual Review of Microbiology · doi:10.1146/annurev.micro.61.080706.093501 · Cited by 621
Biomedical applications of nisin
Shin, J. M., Gwak, J. W., Kamarajan, P., Fenno, J. C., Rickard, A. H., & Kapila, Y. L. (2016) · Journal of Applied Microbiology · doi:10.1111/jam.13033 · Cited by 607
Antimicrobial peptides: Structure, mechanism, and modification
Chen, N., & Jiang, C. (2023) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2023.115377 · Cited by 279
Metadata
- Authors
- Hasper, H. E., de Kruijff, B., & Breukink, E.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-08-18
- Volume
- 43
- Issue
- 36
- Pages
- 11567-11575
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2000693118
- Cited by
- 271
- References
- 26
- FWCI
- 9.46
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Assembly and Stability of Nisin−Lipid II Pores. https://app.cochat.ai/papers/10.1021/bi049476b
- The paper
- Hasper, H. E., de Kruijff, B., & Breukink, E. (2004). Assembly and Stability of Nisin−Lipid II Pores. Biochemistry. https://doi.org/10.1021/bi049476b
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
