The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Assembly and Stability of Nisin−Lipid II Pores
Abstract
The peptide antibiotic nisin was the first reported example of an antibiotic that kills bacteria via targeted pore formation. The specific target of nisin is Lipid II, an essential intermediate in the bacterial cell-wall synthesis. High-affinity binding of the antibiotic to Lipid II is followed by rapid permeabilization of the membrane. Here, we investigated the assembly and stability of nisin-Lipid II pore complexes by means of pyrene fluorescence and circular dichroism. We demonstrated that nisin uses all available Lipid II molecules in the membrane to form pore complexes. The pore complexes have a uniform structure and consist of 8 nisin and 4 Lipid II molecules. Moreover, the pores displayed a remarkable stability, because they were able to resist the solubilization of the membrane environment by mild detergents. Similar experiments with [N20P/M21P]nisin showed that the hinge region is essential for the assembly into stable pore complexes. The new insights were used to propose a refined model for nisin pore formation.
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Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,947
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Rouser, G., Fleischer, S., & Yamamoto, A. (1970) · Lipids · doi:10.1007/bf02531316 · Cited by 3,274
Hope, M. J., Bally, M. B., Webb, G., & Cullis, P. R. (1985) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(85)90521-8 · Cited by 2,076
Woody, R. W. (1995) · Methods in Enzymology · doi:10.1016/0076-6879(95)46006-3 · Cited by 1,034
Applications of the bacteriocin, nisin
Delves-Broughton, J., Blackburn, P., Evans, R. J., & Hugenholtz, J. (1996) · Antonie van Leeuwenhoek · doi:10.1007/bf00399424 · Cited by 972
Use of the Cell Wall Precursor Lipid II by a Pore-Forming Peptide Antibiotic
Breukink, E., Wiedemann, I., Kraaij, C. V., Kuipers, O. P., Sahl, H.-G., & de Kruijff, B. (1999) · Science · doi:10.1126/science.286.5448.2361 · Cited by 814
Self-citationWiedemann, I., Breukink, E., van Kraaij, C., Kuipers, O. P., Bierbaum, G., de Kruijff, B., & Sahl, H.-G. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006770200 · Cited by 790
Self-citationSahl, H.-G., & Bierbaum, G. (1998) · Annual Review of Microbiology · doi:10.1146/annurev.micro.52.1.41 · Cited by 476
Brötz, H., Josten, M., Wiedemann, I., Schneider, U., Götz, F., Bierbaum, G., & Sahl, H. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01065.x · Cited by 421
Lipid II Is an Intrinsic Component of the Pore Induced by Nisin in Bacterial Membranes
Breukink, E., van Heusden, H. E., Vollmerhaus, P. J., Swiezewska, E., Brunner, L., Walker, S., Heck, A. J. R., & de Kruijff, B. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301463200 · Cited by 339
Self-citationBiosynthesis and Biological Activities of Lantibiotics with Unique Post‐Translational Modifications
Sahl, H., Jack, R. W., & Bierbaum, G. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.0827g.x · Cited by 316
Matsuzaki, K., Murase, O., Fujii, N., & Miyajima, K. (1995) · Biochemistry · doi:10.1021/bi00019a033 · Cited by 308
Engineering dehydrated amino acid residues in the antimicrobial peptide nisin.
Kuipers, O. P., Rollema, H. S., Yap, W. M., Boot, H. J., Siezen, R. J., & de Vos, W. M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35771-5 · Cited by 196
Lipid II-Mediated Pore Formation by the Peptide Antibiotic Nisin: a Black Lipid Membrane Study
Wiedemann, I., Benz, R., & Sahl, H.-G. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.10.3259-3261.2004 · Cited by 182
Antimicrobial Peptide Pores in Membranes Detected by Neutron In-Plane Scattering
He, K., Ludtke, S. J., Huang, H. W., & Worcester, D. L. (1995) · Biochemistry · doi:10.1021/bi00048a002 · Cited by 178
Lipid II Induces a Transmembrane Orientation of the Pore-Forming Peptide Lantibiotic Nisin
Hasper, H. E., KRUIJFF, B. D., & Breukink, E. J. (2002) · Biochemistry · doi:10.1021/bi026090x · Cited by 147
Self-citationLantibiotics and microcins: polypeptides with unusual chemical diversity
Jack, R. W., & Jung, G. (2000) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(00)00094-6 · Cited by 136
Sahoo, D., Narayanaswami, V., Kay, C. M., & Ryan, R. O. (2000) · Biochemistry · doi:10.1021/bi992609m · Cited by 129
Gargaud, M., Irvine, W. M., Amils, R., Claeys, P., Cleaves, H. J., Gérin, M., Rouan, D., Spohn, T., Tirard, S., & Viso, M. (2023) · Encyclopedia of Astrobiology · doi:10.1007/978-3-662-65093-6_300086 · Cited by 107
Comparison of the Membrane Association of Two Antimicrobial Peptides, Magainin 2 and Indolicidin
Zhao, H., Mattila, J.-P., Holopainen, J. M., & Kinnunen, P. K. J. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)75938-3 · Cited by 98
Kol, M. A., van Dalen, A., de Kroon, A. I. P. M., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301875200 · Cited by 88
Kol, M. A., de Kroon, A. I. P. M., Rijkers, D. T. S., et al. (2001) · Biochemistry · doi:10.1021/bi010627+ · Cited by 81
The lactose permease of Escherichia coli
Brooker, R. J. (1990) · Research in Microbiology · doi:10.1016/0923-2508(90)90004-a · Cited by 66
Post-translational modifications of lantibiotics
Kupke, T. E., & G�tz, F. (1996) · Antonie van Leeuwenhoek · doi:10.1007/bf00399419 · Cited by 47
Pyrene Excimer Fluorescence as a Probe of Protein Conformational Change
Lehrer, S. S. (1995) · Sub-cellular biochemistry/Subcellular biochemistry · doi:10.1007/978-1-4899-1727-0_5 · Cited by 39
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Stein, T. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04587.x · Cited by 1,507
Biosynthesis and Mode of Action of Lantibiotics
Chatterjee, C., Paul, M., Xie, L., & van der Donk, W. A. (2005) · Chemical Reviews · doi:10.1021/cr030105v · Cited by 718
Lantibiotics: Peptides of Diverse Structure and Function
Willey, J. M., & van der Donk, W. A. (2007) · Annual Review of Microbiology · doi:10.1146/annurev.micro.61.080706.093501 · Cited by 621
Biomedical applications of nisin
Shin, J. M., Gwak, J. W., Kamarajan, P., Fenno, J. C., Rickard, A. H., & Kapila, Y. L. (2016) · Journal of Applied Microbiology · doi:10.1111/jam.13033 · Cited by 607
Antimicrobial peptides: Structure, mechanism, and modification
Chen, N., & Jiang, C. (2023) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2023.115377 · Cited by 279
Metadata
- Authors
- Hasper, H. E., de Kruijff, B., & Breukink, E.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-08-18
- Volume
- 43
- Issue
- 36
- Pages
- 11567-11575
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2000693118
- Cited by
- 271
- References
- 26
- FWCI
- 9.46
- Type
- journal article
- Language
- en
- Open access
- closed
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