Cited by 126Bacterial biofilms and quorum sensingAdvanced Polymer Synthesis and CharacterizationAdvanced Drug Delivery Systems

Nanoparticle (Star Polymer) Delivery of Nitric Oxide Effectively Negates Pseudomonas aeruginosa Biofilm Formation

Duong, Hien T. T., Jung, Kenward, Kutty, Samuel K., Agustina, Sri, Adnan, Nik Nik M., Basuki, Johan S., Kumar, Naresh, Davis, Thomas P. +2 more·2014·Biomacromolecules

Abstract

Biofilms are increasingly recognized as playing a major role in human infectious diseases, as they can form on both living tissues and abiotic surfaces, with serious implications for applications that rely on prolonged exposure to the body such as implantable biomedical devices or catheters. Therefore, there is an urgent need to develop improved therapeutics to effectively eradicate unwanted biofilms. Recently, the biological signaling molecule nitric oxide (NO) was identified as a key regulator of dispersal events in biofilms. In this paper, we report a new class of core cross-linked star polymers designed to store and release nitric oxide, in a controlled way, for the dispersion of biofilms. First, core cross-linked star polymers were prepared by reversible addition-fragmentation chain transfer polymerization (RAFT) via an arm first approach. Poly(oligoethylene methoxy acrylate) chains were synthesized by RAFT polymerization, and then chain extended in the presence of 2-vinyl-4,4-dimethyl-5-oxazolone monomer (VDM) with N,N-methylenebis(acrylamide) employed as a cross-linker to yield functional core cross-linked star polymers. Spermine was successfully attached to the star core by reaction with VDM. Finally, the secondary amine groups were reacted with NO gas to yield NO-core cross-linked star polymers. The core cross-linked star polymers were found to release NO in a controlled, slow delivery in bacterial cultures showing great efficacy in preventing both cell attachment and biofilm formation in Pseudomonas aeruginosa over time via a nontoxic mechanism, confining bacterial growth to the suspended liquid.

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Crossref · 10.1021/bm500422v

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  • Nanoparticles able to decrease Pseudomonas aeruginosa biofilm formation by >70% compared with untreated controls.

    “Furthermore, they found that nanoparticles were able to decrease Pseudomonas aeruginosa biofilm formation by > 70% compared with untreated controls 35 .”

    From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [35]

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Authors
Duong, H. T. T., Jung, K., Kutty, S. K., Agustina, S., Adnan, N. N. M., Basuki, J. S., Kumar, N., Davis, T. P., Barraud, N., & Boyer, C.
Affiliations
  • UNSW Sydney (AU)
  • University of Warwick (GB)
Corresponding author
  • Davis, Thomas P.
  • Barraud, Nicolas
  • Boyer, Cyrille
Venue
Biomacromolecules
Published
2014-06-10
Volume
15
Issue
7
Pages
2583-2589
ISSN
  • 1525-7797 (ISSN-L)
  • 1526-4602
OpenAlex
W2057460683
Cited by
126
References
45
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 9 · 2022: 3 · 2021: 22 · 2020: 11 · 2019: 9 · 2018: 17 · 2017: 10 · 2016: 16 · 2015: 14 · 2014: 2
FWCI
5.74
Type
journal article
Language
en
Open access
closed
Locations
  • Biomacromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
Australian Research Council (DE120101604, FT 120100096)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords
nitric oxide delivery, star polymers, Pseudomonas aeruginosa, biofilm formation, biofilm dispersal, RAFT polymerization, N,N'-methylenebisacrylamide, spermine, controlled release
MeSH terms
Anti-Bacterial Agents, Drug Carriers, Kinetics, Methacrylates, Microbial Sensitivity Tests, Nitric Oxide, Polyethylenes, Pseudomonas aeruginosa, Spectroscopy, Fourier Transform Infrared, Biofilms, Nanoparticles, Polymerization

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