The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Ultraviolet Disinfection of Antibiotic Resistant Bacteria and Their Antibiotic Resistance Genes in Water and Wastewater
Abstract
Disinfection of wastewater treatment plant effluent may be an important barrier for limiting the spread of antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs). While ideally disinfection should destroy ARGs, to prevent horizontal gene transfer to downstream bacteria, little is known about the effect of conventional water disinfection technologies on ARGs. This study examined the potential of UV disinfection to damage four ARGs, mec(A), van(A), tet(A), and amp(C), both in extracellular form and present within a host ARBs: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), Escherichia coli SMS-3-5, and Pseudomonas aeruginosa 01, respectively. An extended amplicon-length quantitative polymerase chain reaction assay was developed to enhance capture of ARG damage events and also to normalize to an equivalent length of target DNA (∼1000 bp) for comparison. It was found that the two Gram-positive ARBs (MRSA and VRE) were more resistant to UV disinfection than the two Gram-negative ARBs (E. coli and P. aeruginosa). The two Gram-positive organisms also possessed smaller total genome sizes, which could also have reduced their susceptibility to UV because of fewer potential pyrimidine dimer targets. An effect of cell type on damage to ARGs was only observed in VRE and P. aeruginosa, the latter potentially because of extracellular polymeric substances. In general, damage of ARGs required much greater UV doses (200-400 mJ/cm² for 3- to 4-log reduction) than ARB inactivation (10-20 mJ/cm² for 4- to 5-log reduction). The proportion of amplifiable ARGs following UV treatment exhibited a strong negative correlation with the number of adjacent thymines (Pearson r < -0.9; p < 0.0001). ARBs surviving UV treatment were negatively correlated with total genome size (Pearson r < -0.9; p < 0.0001) and adjacent cytosines (Pearson r < -0.88; p < 0.0001) but positively correlated with adjacent thymines (Pearson r > 0.85; p < 0.0001). This suggests that formation of thymine dimers is not the sole mechanism of ARB inactivation. Overall, the results indicate that UV is limited in its potential to damage ARGs and other disinfection technologies should be explored.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Origins and evolution of antibiotic resistance
Davies, J., & Davies, D. (2010) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00016-10 · Cited by 5,784
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., Brinkman, F. S. L., Hufnagle, W. O., Kowalik, D. J., Lagrou, M., et al. (2000) · Nature · doi:10.1038/35023079 · Cited by 4,573
Jacoby, G. A. (2009) · Clinical Microbiology Reviews · doi:10.1128/cmr.00036-08 · Cited by 2,165
Antibiotic Resistance Genes as Emerging Contaminants: Studies in Northern Colorado
Pruden, A., Pei, R., Storteboom, H., & Carlson, K. H. (2006) · Environmental Science & Technology · doi:10.1021/es060413l · Cited by 1,949
Self-citationMolecular Mechanisms of Antibacterial Multidrug Resistance
Alekshun, M. N., & Levy, S. B. (2007) · Cell · doi:10.1016/j.cell.2007.03.004 · Cited by 1,647
Diep, B. A., Gill, S. R., Chang, R. F., Phan, T. H., Chen, J. H., Davidson, M. G., Lin, F., Lin, J., Carleton, H. A., Mongodin, E. F., et al. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68231-7 · Cited by 1,633
Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: A review
Hijnen, W. A. M., Beerendonk, E. F., & Medema, G. J. (2006) · Water Research · doi:10.1016/j.watres.2005.10.030 · Cited by 1,591
The antibiotic resistome: the nexus of chemical and genetic diversity
Wright, G. D. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1614 · Cited by 1,365
Oliveira, D. C., & Lencastre, H. D. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.7.2155-2161.2002 · Cited by 1,343
Vancomycin-Resistant Enterococci
Cetinkaya, Y., Falk, P., & Mayhall, C. G. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.4.686 · Cited by 1,163
Pseudomonas genomes: diverse and adaptable
Silby, M. W., Winstanley, C., Godfrey, S. A. C., Levy, S. B., & Jackson, R. W. (2011) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2011.00269.x · Cited by 960
Schwartz, T. (2003) · FEMS Microbiology Ecology · doi:10.1016/s0168-6496(02)00444-0 · Cited by 891
Munir, M., Wong, K., & Xagoraraki, I. (2011) · Water Research · doi:10.1016/j.watres.2010.08.033 · Cited by 881
Multiplex PCR for the detection of tetracycline resistant genes
Ng, L.-K., Martin, I., Alfa, M., & Mulvey, M. (2001) · Molecular and Cellular Probes · doi:10.1006/mcpr.2001.0363 · Cited by 870
Klein, E., Smith, D. L., & Laxminarayan, R. (2007) · Emerging Infectious Diseases · doi:10.3201/eid1312.070629 · Cited by 862
Genetic Recombination in Pseudomonas aeruginosa
HOLLOWAY, B. W. (1955) · Microbiology · doi:10.1099/00221287-13-3-572 · Cited by 650
Kristiansson, E., Fick, J., Janzon, A., Grabic, R., Rutgersson, C., Weijdegård, B., Söderström, H., & Larsson, D. G. J. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017038 · Cited by 566
Tetracycline resistance genes in activated sludge wastewater treatment plants
Auerbach, E. A., Seyfried, E. E., & McMahon, K. D. (2007) · Water Research · doi:10.1016/j.watres.2006.11.045 · Cited by 549
Correlation Between Upstream Human Activities and Riverine Antibiotic Resistance Genes
Pruden, A., Arabi, M., & Storteboom, H. N. (2012) · Environmental Science & Technology · doi:10.1021/es302657r · Cited by 516
Self-citationEvolution and ecology of antibiotic resistance genes
Aminov, R. I., & Mackie, R. I. (2007) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2007.00757.x · Cited by 510
Szczepanowski, R., Linke, B., Krahn, I., Gartemann, K.-H., Gützkow, T., Eichler, W., Pühler, A., & Schlüter, A. (2009) · Microbiology · doi:10.1099/mic.0.028233-0 · Cited by 496
Dodd, M. C. (2012) · Journal of Environmental Monitoring · doi:10.1039/c2em00006g · Cited by 462
Schlüter, A., Szczepanowski, R., Pühler, A., & Top, E. M. (2007) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2007.00074.x · Cited by 399
Characterization of glycopeptide-resistant enterococci from U.S. hospitals
Clark, N. C., Cooksey, R. C., Hill, B. C., Swenson, J. M., & Tenover, F. C. (1993) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.37.11.2311 · Cited by 395
LaPara, T. M., Burch, T. R., McNamara, P. J., Tan, D. T., Yan, M., & Eichmiller, J. J. (2011) · Environmental Science & Technology · doi:10.1021/es202775r · Cited by 370
Ready for a world without antibiotics? The Pensières Antibiotic Resistance Call to Action
Carlet, J., Jarlier, V., Harbarth, S., Voss, A., Goossens, H., Pittet, D., & Forum, T. P. O. T. 3. W. H.-A. I. (2012) · Antimicrobial Resistance and Infection Control · doi:10.1186/2047-2994-1-11 · Cited by 350
Zhang, Y., Marrs, C. F., Simon, C., & Xi, C. (2009) · Science of The Total Environment · doi:10.1016/j.scitotenv.2009.02.013 · Cited by 346
McKinney, C. W., Loftin, K. A., Meyer, M. T., Davis, J. G., & Pruden, A. (2010) · Environmental Science & Technology · doi:10.1021/es9038165 · Cited by 336
Self-citationWang, Y. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh271 · Cited by 310
Volkmann, H., Schwartz, T., Bischoff, P., Kirchen, S., & Obst, U. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2003.10.014 · Cited by 256
Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant
Łuczkiewicz, A., Jankowska, K., Fudala-Książek, S., & Olańczuk-Neyman, K. (2010) · Water Research · doi:10.1016/j.watres.2010.08.007 · Cited by 249
Storteboom, H., Arabi, M., Davis, J. G., Crimi, B., & Pruden, A. (2010) · Environmental Science & Technology · doi:10.1021/es101657s · Cited by 230
Self-citationDumas, J.-L., Delden, C., Perron, K., & Köhler, T. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2005.00008.x · Cited by 224
Synergistic Bactericidal Activity of Ag-TiO2 Nanoparticles in Both Light and Dark Conditions
Li, M., Noriega-Trevino, M. E., Nino-Martinez, N., Marambio-Jones, C., Wang, J., Damoiseaux, R., Ruiz, F., & Hoek, E. M. V. (2011) · Environmental Science & Technology · doi:10.1021/es201675m · Cited by 180
Storteboom, H., Arabi, M., Davis, J. G., Crimi, B., & Pruden, A. (2010) · Environmental Science & Technology · doi:10.1021/es902893f · Cited by 167
Self-citationMethicillin-Resistant Staphylococcus aureus (MRSA) Detected at Four U.S. Wastewater Treatment Plants
Goldstein, R. E. R., Micallef, S. A., Gibbs, S. G., Davis, J. A., He, X., George, A., Kleinfelter, L. M., Schreiber, N. A., Mukherjee, S., Sapkota, A., et al. (2012) · Environmental Health Perspectives · doi:10.1289/ehp.1205436 · Cited by 152
Dalsgaard, A. (2001) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/48.6.827 · Cited by 126
Fricke, W. F., Wright, M. S., Lindell, A. H., Harkins, D. M., Baker‐Austin, C., Ravel, J., & Stepanauskas, R. (2008) · Journal of Bacteriology · doi:10.1128/jb.00661-08 · Cited by 111
Effect of DNA damage on PCR amplification efficiency with the relative threshold cycle method
Sikorsky, J. A., Primerano, D. A., Fenger, T. W., & Denvir, J. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.08.168 · Cited by 111
Nagulapally, S. R., Ahmad, A., Henry, A., Marchin, G. L., Žůrek, L., & Bhandari, A. (2009) · Water Environment Research · doi:10.2175/106143008x304596 · Cited by 92
DNA damage reduces Taq DNA polymerase fidelity and PCR amplification efficiency
Sikorsky, J. A., Primerano, D. A., Fenger, T. W., & Denvir, J. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.01.169 · Cited by 92
Kim, S., Park, H., & Chandran, K. (2010) · Chemosphere · doi:10.1016/j.chemosphere.2009.12.068 · Cited by 75
Prevalence of AmpC over-expression in bloodstream isolates of Pseudomonas aeruginosa
Tam, V. H., Schilling, A. N., LaRocco, M., Gentry, L. O., Lolans, K., Quinn, J. P., & Garey, K. W. (2007) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2006.01674.x · Cited by 64
Cited by
Nnadozie, C. F., & Odume, O. N. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.113067 · Cited by 365
Rizzo, L., Gernjak, W., Krzeminski, P., Malato, S., McArdell, C. S., Perez, J. A. S., Schaar, H., & Fatta-Kassinos, D. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2019.136312 · Cited by 308
He, H., Zhou, P., Shimabuku, K. K., Fang, X., Li, S., Lee, Y., & Dodd, M. C. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b04393 · Cited by 227
Antibiotic resistance spread potential in urban wastewater effluents disinfected by UV/H2O2 process
Ferro, G., Guarino, F., Castiglione, S., & Rizzo, L. (2016) · Science of The Total Environment · doi:10.1016/j.scitotenv.2016.04.047 · Cited by 159
Zammit, I., Marano, R. B. M., Vaiano, V., Cytryn, E., & Rizzo, L. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c01565 · Cited by 71
Metadata
- Authors
- McKinney, C. W., & Pruden, A.
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-11-29
- Volume
- 46
- Issue
- 24
- Pages
- 13393-13400
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2064121493
- Cited by
- 532
- References
- 43
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
