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Cited by 837

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Evidence of Increasing Antibiotic Resistance Gene Abundances in Archived Soils since 1940

Open access
Knapp, Charles W., Dolfing, Jan, Ehlert, Phillip A. I., Graham, David W.·2010·Environmental Science & Technology
DOI 10.1021/es901221x
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Abstract

Mass production and use of antibiotics and antimicrobials in medicine and agriculture have existed for over 60 years, and has substantially benefited public health and agricultural productivity throughout the world. However, there is growing evidence that resistance to antibiotics (AR) is increasing both in benign and pathogenic bacteria, posing an emerging threat to public and environmental health in the future. Although evidence has existed for years from clinical data of increasing AR, almost no quantitative environmental data exist that span increased industrial antibiotic production in the 1950s to the present; i.e., data that might delineate trends in AR potentially valuable for epidemiological studies. To address this critical knowledge gap, we speculated that AR levels might be apparent in historic soil archives as evidenced by antibiotic resistance gene (ARG) abundances over time. Accordingly, DNA was extracted from five long-term soil-series from different locations in The Netherlands that spanned 1940 to 2008, and 16S rRNA gene and 18 ARG abundances from different major antibiotic classes were quantified. Results show that ARG from all classes of antibiotics tested have significantly increased since 1940, but especially within the tetracyclines, with some individual ARG being >15 times more abundant now than in the 1970s. This is noteworthy because waste management procedures have broadly improved and stricter rules on nontherapeutic antibiotic use in agriculture are being promulgated. Although these data are local to The Netherlands, they suggest basal environmental levels of ARG still might be increasing, which has implications to similar locations around the world.

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Metadata

Authors
Knapp, C. W., Dolfing, J., Ehlert, P. A. I., & Graham, D. W.
Venue
Environmental Science & Technology
Publisher
American Chemical Society (ACS)
Published
2009-12-21
Volume
44
Issue
2
Pages
580-587
ISSN
  • 0013-936X (ISSN-L)
  • 1520-5851
DOI
10.1021/es901221x
OpenAlex
W2143445148
Cited by
837
References
30
FWCI
24.78
Type
journal article
Language
en
Open access
green

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