The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Sorting Fluorescent Nanocrystals with DNA
Abstract
Semiconductor nanocrystals with narrow and tunable fluorescence are covalently linked to oligonucleotides. These biocompounds retain the properties of both nanocrystals and DNA. Therefore, different sequences of DNA can be coded with nanocrystals and still preserve their ability to hybridize to their complements. We report the case where four different sequences of DNA are linked to four nanocrystal samples having different colors of emission in the range of 530-640 nm. When the DNA-nanocrystal conjugates are mixed together, it is possible to sort each type of nanoparticle by using hybridization on a defined micrometer-size surface containing the complementary oligonucleotide. Detection of sorting requires only a single excitation source and an epifluorescence microscope. The possibility of directing fluorescent nanocrystals toward specific biological targets and detecting them, combined with their superior photostability compared to organic dyes, opens the way to improved biolabeling experiments, such as gene mapping on a nanometer scale or multicolor microarray analysis.
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Cites
Dabbousi, B. O., Rodriguez-Viejo, J., Mikulec, F. V., Heine, J. R., Mattoussi, H., Ober, R., Jensen, K. F., & Bawendi, M. G. (1997) · The Journal of Physical Chemistry B · doi:10.1021/jp971091y · Cited by 4,229
Cited by
Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
Dubertret, B., Skourides, P., Norris, D. J., Noireaux, V., Brivanlou, A. H., & Libchaber, A. (2002) · Science · doi:10.1126/science.1077194 · Cited by 3,019
Biosensing with Luminescent Semiconductor Quantum Dots
Sapsford, K. E., Pons, T., Medintz, I. L., & Mattoussi, H. (2006) · Sensors · doi:10.3390/s6080925 · Cited by 420
Semisynthetic DNA–Protein Conjugates for Biosensing and Nanofabrication
Niemeyer, C. M. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.200904930 · Cited by 369
Asymmetric Functionalization of Gold Nanoparticles with Oligonucleotides
Xu, X., Rosi, N. L., Wang, Y., Huo, F., & Mirkin, C. A. (2006) · Journal of the American Chemical Society · doi:10.1021/ja061980b · Cited by 337
Metadata
- Authors
- Gerion, D., Parak, W. J., Williams, S. C., Zanchet, D., Micheel, C. M., & Alivisatos, A. P.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-05-25
- Volume
- 124
- Issue
- 24
- Pages
- 7070-7074
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2029956379
- Cited by
- 300
- References
- 1
- FWCI
- 8.32
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
