The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
A FRET-Based Approach to Ratiometric Fluorescence Detection of Hydrogen Peroxide
Abstract
We report the synthesis, properties, and biological applications of Ratio-Peroxyfluor-1 (RPF1), a new ratiometric fluorescent reporter for hydrogen peroxide. RPF1 is comprised of a two-fluorophore cassette, where the spectral overlap between coumarin donor and fluoran/fluorescein acceptor partners can be controlled by the chemoselective peroxide-mediated deprotection of boronic ester pendants on the acceptor dye. RPF1 features good selectivity for hydrogen peroxide over a variety of reactive oxygen species, including superoxide and nitric oxide, a ca. 8-fold increase in fluorescence intensity ratio (lambda517/lambda464) upon H2O2 reaction, and excitation and emission profiles in the visible region. Experiments with viable yeast mitochondria show that RPF1 can monitor and quantify endogenous production of H2O2, establishing the potential utility of this approach for probing peroxide biology in living systems.
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Self-citationFluorescence ratio imaging: a new window into intracellular ionic signaling
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Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species
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ROS production in phagocytes: Why, when, and where?
Dupré-Crochet, S., Erard, M., & Nüβe, O. (2013) · Journal of Leukocyte Biology · doi:10.1189/jlb.1012544 · Cited by 467
Redox Signaling by Reactive Electrophiles and Oxidants
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Metadata
- Authors
- Albers, A. E., Okreglak, V. S., & Chang, C. J.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-07-04
- Volume
- 128
- Issue
- 30
- Pages
- 9640-9641
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2049061652
- Cited by
- 375
- References
- 13
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- Albers, A. E., Okreglak, V. S., & Chang, C. J. (2006). A FRET-Based Approach to Ratiometric Fluorescence Detection of Hydrogen Peroxide. Journal of the American Chemical Society. https://doi.org/10.1021/ja063308k
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