Cited by 356Spectroscopy Techniques in Biomedical and Chemical ResearchClick Chemistry and ApplicationsAdvanced Fluorescence Microscopy Techniques

Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy

Yamakoshi, Hiroyuki, Dodo, Kosuke, Okada, Masaya, Ando, Jun, Palonpon, Almar, Fujita, Katsumasa, Kawata, Satoshi, Sodeoka, Mikiko·2011·Journal of the American Chemical Society

Abstract

Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.

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Crossref · 10.1021/ja108404p

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34 of 34 references verified0 citation errors · 0 not checkable

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  • In the Raman-silent region, only Raman bands from triple bond vibrational modes (e.g., alkyne, cyanide, nitrile) appear.

    “as only Raman bands from triple bond (e.g., alkyne, cyanide, nitrile, etc.) vibrational modes appear 15, 16”

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Authors
Yamakoshi, H., Dodo, K., Okada, M., Ando, J., Palonpon, A., Fujita, K., Kawata, S., & Sodeoka, M.
Affiliations
  • Japan Science and Technology Agency (JP)
  • The University of Osaka (JP)
  • RIKEN Advanced Science Institute (JP)
Corresponding author
Sodeoka, Mikiko
Venue
Journal of the American Chemical Society
Published
2011-03-28
Volume
133
Issue
16
Pages
6102-6105
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
OpenAlex
W2038720171
Cited by
356
References
34
Citations by year
2026: 16 · 2025: 20 · 2024: 14 · 2023: 24 · 2022: 36 · 2021: 48 · 2020: 30 · 2019: 23 · 2018: 26 · 2017: 29 · 2016: 14 · 2015: 23 · 2014: 13 · 2013: 22 · 2012: 11
FWCI
9.73
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the American Chemical Society (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
5-ethynyl-2'-deoxyuridine, cell proliferation, micro-Raman spectroscopy, nuclear localization, living cells
MeSH terms
Alkynes, Deoxyuridine, HeLa Cells, Humans, Microscopy, Spectrum Analysis, Raman, Cell Proliferation

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