Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman Microscopy
Abstract
Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.
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In the Raman-silent region, only Raman bands from triple bond vibrational modes (e.g., alkyne, cyanide, nitrile) appear.
“as only Raman bands from triple bond (e.g., alkyne, cyanide, nitrile, etc.) vibrational modes appear 15, 16”
From Design and evaluation of Raman reporters for the Raman-silent region (2022) · cites this paper as [15]
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- Authors
- Yamakoshi, H., Dodo, K., Okada, M., Ando, J., Palonpon, A., Fujita, K., Kawata, S., & Sodeoka, M.
- Affiliations
- Japan Science and Technology Agency (JP)
- The University of Osaka (JP)
- RIKEN Advanced Science Institute (JP)
- Corresponding author
- Sodeoka, Mikiko
- ORCID
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-03-28
- Volume
- 133
- Issue
- 16
- Pages
- 6102-6105
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2038720171
- Cited by
- 356
- References
- 34
- Citations by year
- 2026: 16 · 2025: 20 · 2024: 14 · 2023: 24 · 2022: 36 · 2021: 48 · 2020: 30 · 2019: 23 · 2018: 26 · 2017: 29 · 2016: 14 · 2015: 23 · 2014: 13 · 2013: 22 · 2012: 11
- FWCI
- 9.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- 5-ethynyl-2'-deoxyuridine, cell proliferation, micro-Raman spectroscopy, nuclear localization, living cells
- MeSH terms
- Alkynes, Deoxyuridine, HeLa Cells, Humans, Microscopy, Spectrum Analysis, Raman, Cell Proliferation
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