Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
Abstract
Imaging with molecular vibrations: The vibrational spectra of biomolecules could in principle image cells and tissue without added markers. Practically, several technical problems need to be overcome to achieve sufficient imaging depths, resolution, and data acquisition speed. Cheng and Xie review emerging bioimaging methods for use in the lab and the clinic. Science , this issue p. 10.1126/science.aaa8870
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The CARS signal from aliphatic C-H2 bonds is specifically enriched in the lipid fraction, in particular in lipid droplets (LDs).
“In the context of lipid analysis, aliphatic C-H2 bonds can be probed, which are specifically enriched in the lipid fraction, in particular in LDs.”
From Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy (2016) · cites this paper as [16]
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- Authors
- Cheng, J.-X., & Xie, X. S.
- Affiliations
- Purdue University West Lafayette (US)
- Harvard University (US)
- Corresponding author
- Cheng, Ji-Xin
- Xie, X. Sunney
- Venue
- Science
- Published
- 2015-11-27
- Volume
- 350
- Issue
- 6264
- Pages
- aaa8870-aaa8870
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2332583381
- Cited by
- 874
- References
- 148
- Citations by year
- 2026: 72 · 2025: 84 · 2024: 93 · 2023: 85 · 2022: 102 · 2021: 101 · 2020: 75 · 2019: 99 · 2018: 62 · 2017: 64 · 2016: 33
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Keywords
- biological systems, detection sensitivity, spatial resolution, imaging depth
- MeSH terms
- Animals, Biology, Gases, Humans, Medicine, Microscopy, Sensitivity and Specificity, Spectrum Analysis, Raman, Vibration, 3T3-L1 Cells, Mice, Molecular Imaging
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