Biological imaging of chemical bonds by stimulated Raman scattering microscopy
Abstract
All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.
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Rapid single-particle chemical imaging of nanoplastics by SRS microscopy
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- Authors
- Hu, F., Shi, L., & Min, W.
- Affiliations
- Columbia University (US)
- Corresponding author
- Min, Wei
- Venue
- Nature Methods
- Published
- 2019-08-30
- Volume
- 16
- Issue
- 9
- Pages
- 830-842
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2970018556
- Cited by
- 392
- References
- 154
- Citations by year
- 2026: 42 · 2025: 58 · 2024: 64 · 2023: 69 · 2022: 61 · 2021: 65 · 2020: 30 · 2019: 2
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Alfred P. Sloan Foundation
- Camille and Henry Dreyfus Foundation
- U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (EB020892, 1DP2EB016573)
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (GM128214)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Keywords
- stimulated Raman scattering microscopy, bioimaging, imaging agents, stimulated Raman spectroscopy
- MeSH terms
- Nonlinear Optical Microscopy, Animals, Humans, Macromolecular Substances, Molecular Imaging
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