Cited by 392Spectroscopy Techniques in Biomedical and Chemical ResearchAdvanced Fluorescence Microscopy TechniquesSpectroscopy and Quantum Chemical Studies

Biological imaging of chemical bonds by stimulated Raman scattering microscopy

Hu, Fanghao, Shi, Lixue, Min, Wei·2019·Nature Methods

Abstract

All molecules consist of chemical bonds, and much can be learned from mapping the spatiotemporal dynamics of these bonds. Since its invention a decade ago, stimulated Raman scattering (SRS) microscopy has become a powerful modality for imaging chemical bonds with high sensitivity, resolution, speed and specificity. We introduce the fundamentals of SRS microscopy and review innovations in SRS microscopes and imaging probes. We highlight examples of exciting biological applications, and share our vision for potential future breakthroughs for this technology.

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Crossref · 10.1038/s41592-019-0538-0

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154 of 154 references verified0 citation errors · 0 not checkable

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Authors
Hu, F., Shi, L., & Min, W.
Affiliations
Columbia University (US)
Corresponding author
Min, Wei
Venue
Nature Methods
Published
2019-08-30
Volume
16
Issue
9
Pages
830-842
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2970018556
Cited by
392
References
154
Citations by year
2026: 42 · 2025: 58 · 2024: 64 · 2023: 69 · 2022: 61 · 2021: 65 · 2020: 30 · 2019: 2
FWCI
4.70
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Alfred P. Sloan Foundation
  • Camille and Henry Dreyfus Foundation
  • U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering (EB020892, 1DP2EB016573)
  • U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (GM128214)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Keywords
stimulated Raman scattering microscopy, bioimaging, imaging agents, stimulated Raman spectroscopy
MeSH terms
Nonlinear Optical Microscopy, Animals, Humans, Macromolecular Substances, Molecular Imaging

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